Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation
Objective: To investigate the effect of Corchorus olitorius aqueous fraction (COAF) on quorum sensing (QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa (PAO1). Methods: The preliminary screening of the anti-QS effect of COAF was performed by evaluating the anti-pathoge...
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Wolters Kluwer Medknow Publications
2021-01-01
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Series: | Asian Pacific Journal of Tropical Biomedicine |
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doaj-ce83bb97adc649f4b05ac0e123fdf5412021-02-03T05:47:57ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222021-01-01112667310.4103/2221-1691.303605Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formationHanan M Al-YousefPerwez AlamZakia KhanamMusarat AminaWafaa H. B. HassanObjective: To investigate the effect of Corchorus olitorius aqueous fraction (COAF) on quorum sensing (QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa (PAO1). Methods: The preliminary screening of the anti-QS effect of COAF was performed by evaluating the anti-pathogenic activity against Chromobacterium violaceum CV026 biosensor strain. Next, the inhibitory effects of COAF on QS-regulated pyocyanin production, proteolytic and elastolytic activities, swarming motility, and biofilm formation were evaluated in PAO1. Results: The results showed that the treatment of COAF significantly decreased the biofilm biomass, attenuated virulence factors, and inhibited swarming motility of PAO1 without affecting the growth of the bacteria in a dose-dependent manner. COAF at 2 000 μg/mL significantly decreased Las B elastase activity in PAO1 culture, exopolysaccharide production, swarming motility, pyocyanin level, and biomass of PAO1 by 55% (P<0.05), 60% (P<0.01), 61% (P<0.01), 65%(P<0.01) and 73% (P<0.01), respectively. In addition, the production of violacein was decreased by 62% (P<0.01) with the treatment of a high dose of COAF. Conclusions: These findings indicate that COAF can be a potential source of anti-QS agents.http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=66;epage=73;aulast=Al-Yousefcorchorus olitorius l; anti-quorum sensing activity; pseudomonas aeruginosa; chromobacterium violaceum; virulence factors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hanan M Al-Yousef Perwez Alam Zakia Khanam Musarat Amina Wafaa H. B. Hassan |
spellingShingle |
Hanan M Al-Yousef Perwez Alam Zakia Khanam Musarat Amina Wafaa H. B. Hassan Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation Asian Pacific Journal of Tropical Biomedicine corchorus olitorius l; anti-quorum sensing activity; pseudomonas aeruginosa; chromobacterium violaceum; virulence factors |
author_facet |
Hanan M Al-Yousef Perwez Alam Zakia Khanam Musarat Amina Wafaa H. B. Hassan |
author_sort |
Hanan M Al-Yousef |
title |
Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation |
title_short |
Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation |
title_full |
Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation |
title_fullStr |
Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation |
title_full_unstemmed |
Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation |
title_sort |
corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Tropical Biomedicine |
issn |
2221-1691 2588-9222 |
publishDate |
2021-01-01 |
description |
Objective: To investigate the effect of Corchorus olitorius aqueous fraction (COAF) on quorum sensing (QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa (PAO1).
Methods: The preliminary screening of the anti-QS effect of COAF was performed by evaluating the anti-pathogenic activity against Chromobacterium violaceum CV026 biosensor strain. Next, the inhibitory effects of COAF on QS-regulated pyocyanin production, proteolytic and elastolytic activities, swarming motility, and biofilm formation were evaluated in PAO1.
Results: The results showed that the treatment of COAF significantly decreased the biofilm biomass, attenuated virulence factors, and inhibited swarming motility of PAO1 without affecting the growth of the bacteria in a dose-dependent manner. COAF at 2 000 μg/mL significantly decreased Las B elastase activity in PAO1 culture, exopolysaccharide production, swarming motility, pyocyanin level, and biomass of PAO1 by 55% (P<0.05), 60% (P<0.01), 61% (P<0.01), 65%(P<0.01) and 73% (P<0.01), respectively. In addition, the production of violacein was decreased by 62% (P<0.01) with the treatment of a high dose of COAF.
Conclusions: These findings indicate that COAF can be a potential source of anti-QS agents. |
topic |
corchorus olitorius l; anti-quorum sensing activity; pseudomonas aeruginosa; chromobacterium violaceum; virulence factors |
url |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=66;epage=73;aulast=Al-Yousef |
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