The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis
Background and rationale. The control of Endothelin-1 (ET-1)-mediated intrahepatic vasoconstriction in cirrhosis is beneficial for the alleviation of relevant complications. Cirrhosis is accompanied by hypogonadism and altered sex hormone status. Besides- sex hormones have vasoactive effects- but it...
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2015-05-01
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doaj-cea54bde40db46dbb48ac519843846502021-06-09T05:53:58ZengElsevierAnnals of Hepatology1665-26812015-05-01143404413The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosisHsin-Ling Ho0Fa-Yauh Lee1Shao-Jung Hsu2Sun-Sang Wang3I-Fang Hsin4Hui-Chun Huang, M.D.5Jing-Yi Lee6Han-Chieh Lin7Shou-Dong Lee8Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, TaiwanDivision of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanDivision of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Department and Institute of Pharmacology, National Yang-Ming University School of Medicine, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan; National Yang-Ming University Hospital, Yilan, TaiwanDepartment of Medical Affair and Planning, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanEndoscopy Center for Diagnosis and Treatment, Taipei Veterans General Hospital, Taipei, Taiwan; Department and Institute of Pharmacology, National Yang-Ming University School of Medicine, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanDivision of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan; Correspondence and reprint request:Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Department and Institute of Pharmacology, National Yang-Ming University School of Medicine, Taipei, TaiwanDivision of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanFaculty of Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan; Division of Gastroenterology, Department of Medicine, Cheng Hsin General Hospital, Taipei, TaiwanBackground and rationale. The control of Endothelin-1 (ET-1)-mediated intrahepatic vasoconstriction in cirrhosis is beneficial for the alleviation of relevant complications. Cirrhosis is accompanied by hypogonadism and altered sex hormone status. Besides- sex hormones have vasoactive effects- but it is unknown if they influence vascular function in cirrhosis. This study aimed to investigate the roles of sex hormones in hepatic vascular reactions to ET-1 in cirrhosis. Liver cirrhosis was induced in Spraque-Dawley male and female rats with common bile duct ligation (BDL). Sham-operated (Sham) rats were controls. On the 43rd day after operations- intrahepatic vascular concentration-response curves to ET-1 were obtained with the following preincubatioins: 1) vehicle; 2) 17β-estradiol; 3) progesterone; 4) testosterone. Livers from sham and BDL rats were dissected for real-time polymerase chain reaction analysis of estrogen- progesterone and testosterone receptors.Results. Compared with sham males perfused with vehicle- sham females presented higher perfusion pressure changes to ET-1 which was reversed only by 17β-estradiol. In cirrhosis- compared with males- 17β-estradiol no longer attenuated vascular responsiveness to ET-1 in females. In females- BDL rats had lower hepatic estrogen receptor α(ERα) mRNA expression than that in sham rats.Conclusions. The sham females showed a stronger intrahepatic vascular constrictive effect to ET-1 than sham males- which could be reversed by 17β-estradiol. However- the influence of 17β-estradiol was lost in cirrhotic females- which may be attributed- at least partly- to intrahepatic ERa down-regulation in females with cirrhosis.http://www.sciencedirect.com/science/article/pii/S1665268119312815Endothelin-1Liver cirrhosisPortal hypertensionSex hormone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hsin-Ling Ho Fa-Yauh Lee Shao-Jung Hsu Sun-Sang Wang I-Fang Hsin Hui-Chun Huang, M.D. Jing-Yi Lee Han-Chieh Lin Shou-Dong Lee |
spellingShingle |
Hsin-Ling Ho Fa-Yauh Lee Shao-Jung Hsu Sun-Sang Wang I-Fang Hsin Hui-Chun Huang, M.D. Jing-Yi Lee Han-Chieh Lin Shou-Dong Lee The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis Annals of Hepatology Endothelin-1 Liver cirrhosis Portal hypertension Sex hormone |
author_facet |
Hsin-Ling Ho Fa-Yauh Lee Shao-Jung Hsu Sun-Sang Wang I-Fang Hsin Hui-Chun Huang, M.D. Jing-Yi Lee Han-Chieh Lin Shou-Dong Lee |
author_sort |
Hsin-Ling Ho |
title |
The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis |
title_short |
The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis |
title_full |
The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis |
title_fullStr |
The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis |
title_full_unstemmed |
The ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis |
title_sort |
ability of 17 β-estradiol to attenuate intrahepatic vasoconstriction to endothelin-1 in female rats is lost in cirrhosis |
publisher |
Elsevier |
series |
Annals of Hepatology |
issn |
1665-2681 |
publishDate |
2015-05-01 |
description |
Background and rationale. The control of Endothelin-1 (ET-1)-mediated intrahepatic vasoconstriction in cirrhosis is beneficial for the alleviation of relevant complications. Cirrhosis is accompanied by hypogonadism and altered sex hormone status. Besides- sex hormones have vasoactive effects- but it is unknown if they influence vascular function in cirrhosis. This study aimed to investigate the roles of sex hormones in hepatic vascular reactions to ET-1 in cirrhosis. Liver cirrhosis was induced in Spraque-Dawley male and female rats with common bile duct ligation (BDL). Sham-operated (Sham) rats were controls. On the 43rd day after operations- intrahepatic vascular concentration-response curves to ET-1 were obtained with the following preincubatioins: 1) vehicle; 2) 17β-estradiol; 3) progesterone; 4) testosterone. Livers from sham and BDL rats were dissected for real-time polymerase chain reaction analysis of estrogen- progesterone and testosterone receptors.Results. Compared with sham males perfused with vehicle- sham females presented higher perfusion pressure changes to ET-1 which was reversed only by 17β-estradiol. In cirrhosis- compared with males- 17β-estradiol no longer attenuated vascular responsiveness to ET-1 in females. In females- BDL rats had lower hepatic estrogen receptor α(ERα) mRNA expression than that in sham rats.Conclusions. The sham females showed a stronger intrahepatic vascular constrictive effect to ET-1 than sham males- which could be reversed by 17β-estradiol. However- the influence of 17β-estradiol was lost in cirrhotic females- which may be attributed- at least partly- to intrahepatic ERa down-regulation in females with cirrhosis. |
topic |
Endothelin-1 Liver cirrhosis Portal hypertension Sex hormone |
url |
http://www.sciencedirect.com/science/article/pii/S1665268119312815 |
work_keys_str_mv |
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