Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements
The Golgi organelle duplicates its protein and lipid content to segregate evenly between two daughter cells after mitosis. However, how Golgi biogenesis is regulated during interphase remains largely unknown. Here we show that messenger RNA (mRNA) expression of <i>GOLPH3</i> and <i>...
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doaj-ceb8b0d78926424f9e8a0ce8112f7c602020-11-25T00:52:34ZengMDPI AGGenes2073-44252019-03-0110324710.3390/genes10030247genes10030247Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter ElementsBeatriz Peñalver-González0Jon Vallejo-Rodríguez1Gartze Mentxaka2Asier Fullaondo3Ainhoa Iglesias-Ara4Seth J. Field5Ana M. Zubiaga6Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, 48080 Bilbao, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, 48080 Bilbao, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, 48080 Bilbao, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, 48080 Bilbao, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, 48080 Bilbao, SpainDivision of Endocrinology and Metabolism, University of California, San Diego, La Jolla, CA 92093, USADepartment of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, 48080 Bilbao, SpainThe Golgi organelle duplicates its protein and lipid content to segregate evenly between two daughter cells after mitosis. However, how Golgi biogenesis is regulated during interphase remains largely unknown. Here we show that messenger RNA (mRNA) expression of <i>GOLPH3</i> and <i>GOLGA2</i>, two genes encoding Golgi proteins, is induced specifically in G1 phase, suggesting a link between cell cycle regulation and Golgi growth. We have examined the role of E2F transcription factors, critical regulators of G1 to S progression of the cell cycle, in the expression of Golgi proteins during interphase. We show that promoter activity for <i>GOLPH3</i>, a Golgi protein that is also oncogenic, is induced by E2F1-3 and repressed by E2F7. Mutation of the E2F motifs present in the <i>GOLPH3</i> promoter region abrogates E2F1-mediated induction of a <i>GOLPH3</i> luciferase reporter construct. Furthermore, we identify a critical CREB/ATF element in the <i>GOLPH3</i> promoter that is required for its steady state and ATF2-induced expression. Interestingly, depletion of <i>GOLPH3</i> with small interfering RNA (siRNA) delays the G1 to S transition in synchronized U2OS cells. Taken together, our results reveal a link between cell cycle regulation and Golgi function, and suggest that E2F-mediated regulation of Golgi genes is required for the timely progression of the cell cycle.https://www.mdpi.com/2073-4425/10/3/247cell cycleE2F factorsGolgi<i>GOLPH3</i>CREB/ATFgene regulation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Beatriz Peñalver-González Jon Vallejo-Rodríguez Gartze Mentxaka Asier Fullaondo Ainhoa Iglesias-Ara Seth J. Field Ana M. Zubiaga |
spellingShingle |
Beatriz Peñalver-González Jon Vallejo-Rodríguez Gartze Mentxaka Asier Fullaondo Ainhoa Iglesias-Ara Seth J. Field Ana M. Zubiaga Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements Genes cell cycle E2F factors Golgi <i>GOLPH3</i> CREB/ATF gene regulation |
author_facet |
Beatriz Peñalver-González Jon Vallejo-Rodríguez Gartze Mentxaka Asier Fullaondo Ainhoa Iglesias-Ara Seth J. Field Ana M. Zubiaga |
author_sort |
Beatriz Peñalver-González |
title |
Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements |
title_short |
Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements |
title_full |
Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements |
title_fullStr |
Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements |
title_full_unstemmed |
Golgi Oncoprotein <i>GOLPH3</i> Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements |
title_sort |
golgi oncoprotein <i>golph3</i> gene expression is regulated by functional e2f and creb/atf promoter elements |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2019-03-01 |
description |
The Golgi organelle duplicates its protein and lipid content to segregate evenly between two daughter cells after mitosis. However, how Golgi biogenesis is regulated during interphase remains largely unknown. Here we show that messenger RNA (mRNA) expression of <i>GOLPH3</i> and <i>GOLGA2</i>, two genes encoding Golgi proteins, is induced specifically in G1 phase, suggesting a link between cell cycle regulation and Golgi growth. We have examined the role of E2F transcription factors, critical regulators of G1 to S progression of the cell cycle, in the expression of Golgi proteins during interphase. We show that promoter activity for <i>GOLPH3</i>, a Golgi protein that is also oncogenic, is induced by E2F1-3 and repressed by E2F7. Mutation of the E2F motifs present in the <i>GOLPH3</i> promoter region abrogates E2F1-mediated induction of a <i>GOLPH3</i> luciferase reporter construct. Furthermore, we identify a critical CREB/ATF element in the <i>GOLPH3</i> promoter that is required for its steady state and ATF2-induced expression. Interestingly, depletion of <i>GOLPH3</i> with small interfering RNA (siRNA) delays the G1 to S transition in synchronized U2OS cells. Taken together, our results reveal a link between cell cycle regulation and Golgi function, and suggest that E2F-mediated regulation of Golgi genes is required for the timely progression of the cell cycle. |
topic |
cell cycle E2F factors Golgi <i>GOLPH3</i> CREB/ATF gene regulation |
url |
https://www.mdpi.com/2073-4425/10/3/247 |
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