A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes

A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes

Reprogramming has been shown to involve EMT-MET; however, its role in cell differentiation is unclear. Here the authors show that duringin vitrodifferentiation of hepatocytes, Activin A-induced formation of definitive endoderm requires EMT mediated by TGFβ signalling, followed by a MET process.

Bibliographic Details
Main Authors: Qiuhong Li, Andrew P. Hutchins, Yong Chen, Shengbiao Li, Yongli Shan, Baojian Liao, Dejin Zheng, Xi Shi, Yinxiong Li, Wai-Yee Chan, Guangjin Pan, Shicheng Wei, Xiaodong Shu, Duanqing Pei
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15166
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author Qiuhong Li
Andrew P. Hutchins
Yong Chen
Shengbiao Li
Yongli Shan
Baojian Liao
Dejin Zheng
Xi Shi
Yinxiong Li
Wai-Yee Chan
Guangjin Pan
Shicheng Wei
Xiaodong Shu
Duanqing Pei
spellingShingle Qiuhong Li
Andrew P. Hutchins
Yong Chen
Shengbiao Li
Yongli Shan
Baojian Liao
Dejin Zheng
Xi Shi
Yinxiong Li
Wai-Yee Chan
Guangjin Pan
Shicheng Wei
Xiaodong Shu
Duanqing Pei
A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
Nature Communications
author_facet Qiuhong Li
Andrew P. Hutchins
Yong Chen
Shengbiao Li
Yongli Shan
Baojian Liao
Dejin Zheng
Xi Shi
Yinxiong Li
Wai-Yee Chan
Guangjin Pan
Shicheng Wei
Xiaodong Shu
Duanqing Pei
author_sort Qiuhong Li
title A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
title_short A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
title_full A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
title_fullStr A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
title_full_unstemmed A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
title_sort sequential emt-met mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-05-01
description Reprogramming has been shown to involve EMT-MET; however, its role in cell differentiation is unclear. Here the authors show that duringin vitrodifferentiation of hepatocytes, Activin A-induced formation of definitive endoderm requires EMT mediated by TGFβ signalling, followed by a MET process.
url https://doi.org/10.1038/ncomms15166
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spelling doaj-cec18cac0b1e41b2b6dcfc93927833212021-05-11T07:35:43ZengNature Publishing GroupNature Communications2041-17232017-05-018111210.1038/ncomms15166A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytesQiuhong Li0Andrew P. Hutchins1Yong Chen2Shengbiao Li3Yongli Shan4Baojian Liao5Dejin Zheng6Xi Shi7Yinxiong Li8Wai-Yee Chan9Guangjin Pan10Shicheng Wei11Xiaodong Shu12Duanqing Pei13CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCentral Laboratory, School and Hospital of Stomatology, Peking UniversityCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesReprogramming has been shown to involve EMT-MET; however, its role in cell differentiation is unclear. Here the authors show that duringin vitrodifferentiation of hepatocytes, Activin A-induced formation of definitive endoderm requires EMT mediated by TGFβ signalling, followed by a MET process.https://doi.org/10.1038/ncomms15166

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