Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i>
In most diploid organisms, mating is a prerequisite for reproduction and, thus, critical to the maintenance of their population and the perpetuation of the species. Besides the importance of understanding the fundamentals of reproduction, targeting the reproductive success of a pest insect is also a...
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doaj-cedaa4dd53ad4d18961d9c3d17b11b442021-03-01T00:01:16ZengMDPI AGGenes2073-44252021-02-011235535510.3390/genes12030355Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i>Maria-Eleni Gregoriou0Martin Reczko1Evdoxia G. Kakani2Konstantina T. Tsoumani3Kostas D. Mathiopoulos4Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, GreeceInstitute for Fundamental Biomedical Science, Biomedical Sciences Research Centre “Alexander Fleming”, 16672 Vari, GreeceDepartment of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 665 Huntington Avenue, Building 1, Room 103, Boston, MA 02115, USADepartment of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, GreeceIn most diploid organisms, mating is a prerequisite for reproduction and, thus, critical to the maintenance of their population and the perpetuation of the species. Besides the importance of understanding the fundamentals of reproduction, targeting the reproductive success of a pest insect is also a promising method for its control, as a possible manipulation of the reproductive system could affect its destructive activity. Here, we used an integrated approach for the elucidation of the reproductive system and mating procedures of the olive fruit fly, <i>Bactrocera oleae</i>. Initially, we performed a RNAseq analysis in reproductive tissues of virgin and mated insects. A comparison of the transcriptomes resulted in the identification of genes that are differentially expressed after mating. Functional annotation of the genes showed an alteration in the metabolic, catalytic, and cellular processes after mating. Moreover, a functional analysis through RNAi silencing of two differentially expressed genes, <i>yellow-g</i> and <i>troponin C</i>, resulted in a significantly reduced oviposition rate. This study provided a foundation for future investigations into the olive fruit fly’s reproductive biology to the development of new exploitable tools for its control.https://www.mdpi.com/2073-4425/12/3/355<i>Bactrocera oleae</i>reproductionoviposition rateRNAi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria-Eleni Gregoriou Martin Reczko Evdoxia G. Kakani Konstantina T. Tsoumani Kostas D. Mathiopoulos |
spellingShingle |
Maria-Eleni Gregoriou Martin Reczko Evdoxia G. Kakani Konstantina T. Tsoumani Kostas D. Mathiopoulos Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i> Genes <i>Bactrocera oleae</i> reproduction oviposition rate RNAi |
author_facet |
Maria-Eleni Gregoriou Martin Reczko Evdoxia G. Kakani Konstantina T. Tsoumani Kostas D. Mathiopoulos |
author_sort |
Maria-Eleni Gregoriou |
title |
Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i> |
title_short |
Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i> |
title_full |
Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i> |
title_fullStr |
Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i> |
title_full_unstemmed |
Decoding the Reproductive System of the Olive Fruit Fly, <i>Bactrocera oleae</i> |
title_sort |
decoding the reproductive system of the olive fruit fly, <i>bactrocera oleae</i> |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2021-02-01 |
description |
In most diploid organisms, mating is a prerequisite for reproduction and, thus, critical to the maintenance of their population and the perpetuation of the species. Besides the importance of understanding the fundamentals of reproduction, targeting the reproductive success of a pest insect is also a promising method for its control, as a possible manipulation of the reproductive system could affect its destructive activity. Here, we used an integrated approach for the elucidation of the reproductive system and mating procedures of the olive fruit fly, <i>Bactrocera oleae</i>. Initially, we performed a RNAseq analysis in reproductive tissues of virgin and mated insects. A comparison of the transcriptomes resulted in the identification of genes that are differentially expressed after mating. Functional annotation of the genes showed an alteration in the metabolic, catalytic, and cellular processes after mating. Moreover, a functional analysis through RNAi silencing of two differentially expressed genes, <i>yellow-g</i> and <i>troponin C</i>, resulted in a significantly reduced oviposition rate. This study provided a foundation for future investigations into the olive fruit fly’s reproductive biology to the development of new exploitable tools for its control. |
topic |
<i>Bactrocera oleae</i> reproduction oviposition rate RNAi |
url |
https://www.mdpi.com/2073-4425/12/3/355 |
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