The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation
To access the fermentative response of equine caecal microbial population to nitrogen availability, an in vitro study was conducted using caecal contents provided with adequate energy sources and nitrogen as limiting nutrient. Two nitrogen (N) sources were provided, protein (casein) and non-protein...
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doaj-ceeab1a256ed408690dda24059fd289c2021-06-06T04:47:28ZengElsevierAnimal1751-73112012-01-016710961102The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentationA.S. Santos0L.M.M. Ferreira1W. Martin-Rosset2M. Cotovio3F. Silva4R.N. Bennett5J.W. Cone6R.J.B. Bessa7M.A.M. Rodrigues8Animal Production Group, Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, PO Box 1013, 5001-801 Vila Real, PortugalAnimal Production Group, Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, PO Box 1013, 5001-801 Vila Real, PortugalInstitut National de la Recherche Agronomique, Center of Research of Clermont-Ferrand, Theix, 63122 Saint-Gene's-Champanelle, FranceAnimal Production Group, Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, PO Box 1013, 5001-801 Vila Real, PortugalAnimal Production Group, Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, PO Box 1013, 5001-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, PO Box 1013, 5001-801 Vila Real, PortugalAnimal Nutrition Group, Department of Animal Science, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The NetherlandsInterdisciplinary Centre of Research in Animal Health (CIISA), Faculdade de Medicina Veterinária, Technical University of Lisbon, Lisboa, PortugalAnimal Production Group, Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, PO Box 1013, 5001-801 Vila Real, PortugalTo access the fermentative response of equine caecal microbial population to nitrogen availability, an in vitro study was conducted using caecal contents provided with adequate energy sources and nitrogen as limiting nutrient. Two nitrogen (N) sources were provided, protein (casein) and non-protein (urea). Caecal fluid, taken from three cannulated horses receiving a hay–concentrate diet, was mixed with a N-free buffer–mineral solution. The influence of four N levels (3.7, 6.3, 12.5 or 25 mg of N in casein or urea) was studied using the gas production technique. Total volatile fatty acids (VFA), NH3-N and gas production were measured after a 24-h incubation period. Microbial biomass was estimated using adenine and guanine bases as internal markers, and ATP production was estimated stoichiometrically. Microbial growth efficiency (YATP) and gas efficiency (Egas) were estimated. Fermentation with casein as the sole N source was generally characterized by lower total VFA, NH3-N, total gas production and higher acetate : propionate (A : P) ratio and YATP than with urea. Results herein presented indicate that, under these in vitro conditions, caecal microbial population does in fact use urea N, but less efficiently than casein in terms of microbial growth.http://www.sciencedirect.com/science/article/pii/S1751731111002527caecal fermentationcaseinureain vitroequine hindgut |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A.S. Santos L.M.M. Ferreira W. Martin-Rosset M. Cotovio F. Silva R.N. Bennett J.W. Cone R.J.B. Bessa M.A.M. Rodrigues |
spellingShingle |
A.S. Santos L.M.M. Ferreira W. Martin-Rosset M. Cotovio F. Silva R.N. Bennett J.W. Cone R.J.B. Bessa M.A.M. Rodrigues The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation Animal caecal fermentation casein urea in vitro equine hindgut |
author_facet |
A.S. Santos L.M.M. Ferreira W. Martin-Rosset M. Cotovio F. Silva R.N. Bennett J.W. Cone R.J.B. Bessa M.A.M. Rodrigues |
author_sort |
A.S. Santos |
title |
The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation |
title_short |
The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation |
title_full |
The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation |
title_fullStr |
The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation |
title_full_unstemmed |
The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation |
title_sort |
influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation |
publisher |
Elsevier |
series |
Animal |
issn |
1751-7311 |
publishDate |
2012-01-01 |
description |
To access the fermentative response of equine caecal microbial population to nitrogen availability, an in vitro study was conducted using caecal contents provided with adequate energy sources and nitrogen as limiting nutrient. Two nitrogen (N) sources were provided, protein (casein) and non-protein (urea). Caecal fluid, taken from three cannulated horses receiving a hay–concentrate diet, was mixed with a N-free buffer–mineral solution. The influence of four N levels (3.7, 6.3, 12.5 or 25 mg of N in casein or urea) was studied using the gas production technique. Total volatile fatty acids (VFA), NH3-N and gas production were measured after a 24-h incubation period. Microbial biomass was estimated using adenine and guanine bases as internal markers, and ATP production was estimated stoichiometrically. Microbial growth efficiency (YATP) and gas efficiency (Egas) were estimated. Fermentation with casein as the sole N source was generally characterized by lower total VFA, NH3-N, total gas production and higher acetate : propionate (A : P) ratio and YATP than with urea. Results herein presented indicate that, under these in vitro conditions, caecal microbial population does in fact use urea N, but less efficiently than casein in terms of microbial growth. |
topic |
caecal fermentation casein urea in vitro equine hindgut |
url |
http://www.sciencedirect.com/science/article/pii/S1751731111002527 |
work_keys_str_mv |
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