Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression
Ethylene regulates plant abiotic stress responses and tolerances, and ethylene-insensitive3 (EIN3)/EIN3-like (EIL) proteins are the key components of ethylene signal transduction. Although the functions of EIN3/EIL proteins in response to abiotic stresses have been investigated in model plants, litt...
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doaj-cef8d0909590441ba2ad8b6a74a288a42020-11-25T00:56:27ZengPeerJ Inc.PeerJ2167-83592019-02-017e639110.7717/peerj.6391Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expressionChangying Liu0Jun Li1Panpan Zhu2Jian Yu3Jiamin Hou4Chuanhong Wang5Dingpei Long6Maode Yu7Aichun Zhao8State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, ChinaGuiyang University of Chinese Medicine, Guiyang, ChinaState Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, ChinaThe National Key Engineering Lab of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, ChinaEthylene regulates plant abiotic stress responses and tolerances, and ethylene-insensitive3 (EIN3)/EIN3-like (EIL) proteins are the key components of ethylene signal transduction. Although the functions of EIN3/EIL proteins in response to abiotic stresses have been investigated in model plants, little is known in non-model plants, including mulberry (Morus L.), which is an economically important perennial woody plant. We functionally characterized a gene encoding an EIN3-like protein from mulberry, designated as MnEIL3. A quantitative real-time PCR analysis demonstrated that the expression of MnEIL3 could be induced in roots and shoot by salt and drought stresses. Arabidopsis overexpressing MnEIL3 exhibited an enhanced tolerance to salt and drought stresses. MnEIL3 overexpression in Arabidopsis significantly upregulated the transcript abundances of ethylene biosynthetic genes. Furthermore, MnEIL3 enhanced the activities of the MnACO1 and MnACS1 promoters, which respond to salt and drought stresses. Thus, MnEIL3 may play important roles in tolerance to abiotic stresses and the expression of ethylene biosynthetic genes.https://peerj.com/articles/6391.pdfMnEIL3MulberryArabidopsisAbiotic stressesSalt stressDrought |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Changying Liu Jun Li Panpan Zhu Jian Yu Jiamin Hou Chuanhong Wang Dingpei Long Maode Yu Aichun Zhao |
spellingShingle |
Changying Liu Jun Li Panpan Zhu Jian Yu Jiamin Hou Chuanhong Wang Dingpei Long Maode Yu Aichun Zhao Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression PeerJ MnEIL3 Mulberry Arabidopsis Abiotic stresses Salt stress Drought |
author_facet |
Changying Liu Jun Li Panpan Zhu Jian Yu Jiamin Hou Chuanhong Wang Dingpei Long Maode Yu Aichun Zhao |
author_sort |
Changying Liu |
title |
Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression |
title_short |
Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression |
title_full |
Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression |
title_fullStr |
Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression |
title_full_unstemmed |
Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression |
title_sort |
mulberry eil3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2019-02-01 |
description |
Ethylene regulates plant abiotic stress responses and tolerances, and ethylene-insensitive3 (EIN3)/EIN3-like (EIL) proteins are the key components of ethylene signal transduction. Although the functions of EIN3/EIL proteins in response to abiotic stresses have been investigated in model plants, little is known in non-model plants, including mulberry (Morus L.), which is an economically important perennial woody plant. We functionally characterized a gene encoding an EIN3-like protein from mulberry, designated as MnEIL3. A quantitative real-time PCR analysis demonstrated that the expression of MnEIL3 could be induced in roots and shoot by salt and drought stresses. Arabidopsis overexpressing MnEIL3 exhibited an enhanced tolerance to salt and drought stresses. MnEIL3 overexpression in Arabidopsis significantly upregulated the transcript abundances of ethylene biosynthetic genes. Furthermore, MnEIL3 enhanced the activities of the MnACO1 and MnACS1 promoters, which respond to salt and drought stresses. Thus, MnEIL3 may play important roles in tolerance to abiotic stresses and the expression of ethylene biosynthetic genes. |
topic |
MnEIL3 Mulberry Arabidopsis Abiotic stresses Salt stress Drought |
url |
https://peerj.com/articles/6391.pdf |
work_keys_str_mv |
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