Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae)
Scadoxus multiflorus Martyn, 1795 is an ornamental plant with brilliantly colored flowers. Even though its chromosomes are rather large, there is no karyotype description reported so far. Therefore, conventional and molecular cytogenetic studies including fluorescence in...
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doaj-cf027522c76d4a31a2d6115e50197e502020-11-24T22:29:06ZengPensoft PublishersComparative Cytogenetics1993-07711993-078X2016-11-0110463764610.3897/CompCytogen.v10i4.99589958Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae)Pansa Monkheang0Arunrat Chaveerach1Runglawan Sudmoon2Tawatchai Tanee3Khon Kaen UniversityKhon Kaen UniversityKhon Kaen UniversityKhon Kaen University (Khon Kaen) and Mahasarakham University Scadoxus multiflorus Martyn, 1795 is an ornamental plant with brilliantly colored flowers. Even though its chromosomes are rather large, there is no karyotype description reported so far. Therefore, conventional and molecular cytogenetic studies including fluorescence in situ hybridization (FISH) with 45S and 5S rDNA, and human telomere sequence (TTAGGG)n probes (Arabidopsis-type telomere probes yielded negative results) were carried out. The chromosome number is as reported previously, 2n = 18. The nine chromosome pairs include two large submetacentric, five large acrocentric, one medium acrocentric, two small metacentric and eight small submetacentric chromosomes. Hybridization sites of the 45S rDNA signals were on the short arm ends of chromosomes #1, #3 and #8, while 5S rDNA signals appeared on the long arm of chromosome 3, in one homologue as a double signal. The telomere signals were restricted to all chromosome ends. Three chromosome pairs could be newly identified, chromosome pair 3 by 5S rDNA and chromosomes #1, #3 and #8 by 45S rDNA loci. In addition to new information about rDNA locations we show that the ends of S. multiflorus chromosomes harbor human instead of Arabidopsis-type telomere sequences. Overall, the S. multiflorus karyotype presents chromosomal heteromorphy concerning size, shape and 45S and 5S rDNA positioning. As Scadoxus Rafinesque, 1838 and related species are poorly studied on chromosomal level the here presented data is important for better understanding of evolution in Amaryllidaceae. http://compcytogen.pensoft.net/articles.php?id=9958 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pansa Monkheang Arunrat Chaveerach Runglawan Sudmoon Tawatchai Tanee |
spellingShingle |
Pansa Monkheang Arunrat Chaveerach Runglawan Sudmoon Tawatchai Tanee Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae) Comparative Cytogenetics |
author_facet |
Pansa Monkheang Arunrat Chaveerach Runglawan Sudmoon Tawatchai Tanee |
author_sort |
Pansa Monkheang |
title |
Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae) |
title_short |
Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae) |
title_full |
Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae) |
title_fullStr |
Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae) |
title_full_unstemmed |
Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae) |
title_sort |
karyotypic features including organizations of the 5s, 45s rdna loci and telomeres of scadoxus multiflorus (amaryllidaceae) |
publisher |
Pensoft Publishers |
series |
Comparative Cytogenetics |
issn |
1993-0771 1993-078X |
publishDate |
2016-11-01 |
description |
Scadoxus multiflorus Martyn, 1795 is an ornamental plant with brilliantly colored flowers. Even though its chromosomes are rather large, there is no karyotype description reported so far. Therefore, conventional and molecular cytogenetic studies including fluorescence in situ hybridization (FISH) with 45S and 5S rDNA, and human telomere sequence (TTAGGG)n probes (Arabidopsis-type telomere probes yielded negative results) were carried out. The chromosome number is as reported previously, 2n = 18. The nine chromosome pairs include two large submetacentric, five large acrocentric, one medium acrocentric, two small metacentric and eight small submetacentric chromosomes. Hybridization sites of the 45S rDNA signals were on the short arm ends of chromosomes #1, #3 and #8, while 5S rDNA signals appeared on the long arm of chromosome 3, in one homologue as a double signal. The telomere signals were restricted to all chromosome ends. Three chromosome pairs could be newly identified, chromosome pair 3 by 5S rDNA and chromosomes #1, #3 and #8 by 45S rDNA loci. In addition to new information about rDNA locations we show that the ends of S. multiflorus chromosomes harbor human instead of Arabidopsis-type telomere sequences. Overall, the S. multiflorus karyotype presents chromosomal heteromorphy concerning size, shape and 45S and 5S rDNA positioning. As Scadoxus Rafinesque, 1838 and related species are poorly studied on chromosomal level the here presented data is important for better understanding of evolution in Amaryllidaceae.
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url |
http://compcytogen.pensoft.net/articles.php?id=9958 |
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