KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects
The protective effect of KIOM-4, a mixture of plant extracts, was examined against streptozotocin (STZ)-induced mitochondrial oxidative stress in rat pancreatic β-cells (RINm5F). KIOM-4 scavenged superoxide and hydroxyl radicals generated by xanthine/xanthine oxidase and Fenton reaction (FeSO4/H2O2)...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2011-01-01
|
Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1093/ecam/neq007 |
id |
doaj-cf05c27d02e3403d8c10e4255f9af384 |
---|---|
record_format |
Article |
spelling |
doaj-cf05c27d02e3403d8c10e4255f9af3842020-11-24T22:27:17ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882011-01-01201110.1093/ecam/neq007978682KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant EffectsKyoung Ah Kang0Jin Sook Kim1Rui Zhang2Mei Jing Piao3Young Hee Maeng4Mi Young Kang5In Kyung Lee6Bum Joon Kim7Jin Won Hyun8School of Medicine, Jeju National University, Jeju-si 690-756, Republic of KoreaDiabetic Complication Research Center, Division of Traditional Korean Medicine Integrated Research, Korea Institute of Oriental Medicine, Daejeon, Republic of KoreaSchool of Medicine, Jeju National University, Jeju-si 690-756, Republic of KoreaSchool of Medicine, Jeju National University, Jeju-si 690-756, Republic of KoreaSchool of Medicine, Jeju National University, Jeju-si 690-756, Republic of KoreaDepartment of Biomaterials, DNA Repair Center, Chosun University, Gwangju, Republic of KoreaDepartment of Microbiology and Caner Research Institute, Seoul National University College of Medicine, Seoul, Republic of KoreaDepartment of Microbiology and Caner Research Institute, Seoul National University College of Medicine, Seoul, Republic of KoreaSchool of Medicine, Jeju National University, Jeju-si 690-756, Republic of KoreaThe protective effect of KIOM-4, a mixture of plant extracts, was examined against streptozotocin (STZ)-induced mitochondrial oxidative stress in rat pancreatic β-cells (RINm5F). KIOM-4 scavenged superoxide and hydroxyl radicals generated by xanthine/xanthine oxidase and Fenton reaction (FeSO4/H2O2), respectively, in a cell-free chemical system. In addition, a marked increase in mitochondrial reactive oxygen species (ROS) was observed in STZ-induced diabetic cells; this increase was attenuated by KIOM-4 treatment. Mitochondrial manganese superoxide dismutase (Mn SOD) activity and protein expression were down-regulated by STZ treatment and up-regulated by KIOM-4 treatment. In addition, NF-E2 related factor 2 (Nrf2), a transcription factor for Mn SOD, was up-regulated by KIOM-4. KIOM-4 prevented STZ-induced mitochondrial lipid peroxidation, protein carbonyl and DNA modification. Moreover, KIOM-4 treatment restored the loss of mitochondrial membrane potential (Δψ) that was induced by STZ treatment, and inhibited the translocation of cytochrome c from the mitochondria to the cytosol. In addition, KIOM-4 treatment elevated the level of ATP, succinate dehydrogenase activity and insulin level, which were reduced by STZ treatment. These results suggest that KIOM-4 exhibits a protective effect through its antioxidant effect and the attenuation of mitochondrial dysfunction in STZ-induced diabetic cells.http://dx.doi.org/10.1093/ecam/neq007 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyoung Ah Kang Jin Sook Kim Rui Zhang Mei Jing Piao Young Hee Maeng Mi Young Kang In Kyung Lee Bum Joon Kim Jin Won Hyun |
spellingShingle |
Kyoung Ah Kang Jin Sook Kim Rui Zhang Mei Jing Piao Young Hee Maeng Mi Young Kang In Kyung Lee Bum Joon Kim Jin Won Hyun KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects Evidence-Based Complementary and Alternative Medicine |
author_facet |
Kyoung Ah Kang Jin Sook Kim Rui Zhang Mei Jing Piao Young Hee Maeng Mi Young Kang In Kyung Lee Bum Joon Kim Jin Won Hyun |
author_sort |
Kyoung Ah Kang |
title |
KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects |
title_short |
KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects |
title_full |
KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects |
title_fullStr |
KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects |
title_full_unstemmed |
KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects |
title_sort |
kiom-4 protects against oxidative stress-induced mitochondrial damage in pancreatic β-cells via its antioxidant effects |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2011-01-01 |
description |
The protective effect of KIOM-4, a mixture of plant extracts, was examined against streptozotocin (STZ)-induced mitochondrial oxidative stress in rat pancreatic β-cells (RINm5F). KIOM-4 scavenged superoxide and hydroxyl radicals generated by xanthine/xanthine oxidase and Fenton reaction (FeSO4/H2O2), respectively, in a cell-free chemical system. In addition, a marked increase in mitochondrial reactive oxygen species (ROS) was observed in STZ-induced diabetic cells; this increase was attenuated by KIOM-4 treatment. Mitochondrial manganese superoxide dismutase (Mn SOD) activity and protein expression were down-regulated by STZ treatment and up-regulated by KIOM-4 treatment. In addition, NF-E2 related factor 2 (Nrf2), a transcription factor for Mn SOD, was up-regulated by KIOM-4. KIOM-4 prevented STZ-induced mitochondrial lipid peroxidation, protein carbonyl and DNA modification. Moreover, KIOM-4 treatment restored the loss of mitochondrial membrane potential (Δψ) that was induced by STZ treatment, and inhibited the translocation of cytochrome c from the mitochondria to the cytosol. In addition, KIOM-4 treatment elevated the level of ATP, succinate dehydrogenase activity and insulin level, which were reduced by STZ treatment. These results suggest that KIOM-4 exhibits a protective effect through its antioxidant effect and the attenuation of mitochondrial dysfunction in STZ-induced diabetic cells. |
url |
http://dx.doi.org/10.1093/ecam/neq007 |
work_keys_str_mv |
AT kyoungahkang kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT jinsookkim kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT ruizhang kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT meijingpiao kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT youngheemaeng kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT miyoungkang kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT inkyunglee kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT bumjoonkim kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects AT jinwonhyun kiom4protectsagainstoxidativestressinducedmitochondrialdamageinpancreaticbcellsviaitsantioxidanteffects |
_version_ |
1725750509871038464 |