Dysbiosis of the microbiome in gastric carcinogenesis
Abstract The gastric microbiome has been proposed as an etiological factor in gastric carcinogenesis. We compared the gastric microbiota in subjects presenting with gastric cancer (GC, n = 12) and controls (functional dyspepsia (FD), n = 20) from a high GC risk population in Singapore and Malaysia....
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2017-11-01
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doaj-cf0b04e838fd4269857bc5814d673cd32020-12-08T00:17:41ZengNature Publishing GroupScientific Reports2045-23222017-11-01711910.1038/s41598-017-16289-2Dysbiosis of the microbiome in gastric carcinogenesisNatalia Castaño-Rodríguez0Khean-Lee Goh1Kwong Ming Fock2Hazel M. Mitchell3Nadeem O. Kaakoush4School of Biotechnology and Biomolecular Sciences, UNSW SydneyDepartment of Medicine, Faculty of Medicine, University of MalayaDivision of Gastroenterology, Department of Medicine, Changi General HospitalSchool of Biotechnology and Biomolecular Sciences, UNSW SydneySchool of Medical Sciences, UNSW SydneyAbstract The gastric microbiome has been proposed as an etiological factor in gastric carcinogenesis. We compared the gastric microbiota in subjects presenting with gastric cancer (GC, n = 12) and controls (functional dyspepsia (FD), n = 20) from a high GC risk population in Singapore and Malaysia. cDNA from 16S rRNA transcripts were amplified (515F-806R) and sequenced using Illumina MiSeq 2 × 250 bp chemistry. Increased richness and phylogenetic diversity but not Shannon’s diversity was found in GC as compared to controls. nMDS clustered GC and FD subjects separately, with PERMANOVA confirming a significant difference between the groups. H. pylori serological status had a significant impact on gastric microbiome α-diversity and composition. Several bacterial taxa were enriched in GC, including Lactococcus, Veilonella, and Fusobacteriaceae (Fusobacterium and Leptotrichia). Prediction of bacterial metabolic contribution indicated that serological status had a significant impact on metabolic function, while carbohydrate digestion and pathways were enriched in GC. Our findings highlight three mechanisms of interest in GC, including enrichment of pro-inflammatory oral bacterial species, increased abundance of lactic acid producing bacteria, and enrichment of short chain fatty acid production pathways.https://doi.org/10.1038/s41598-017-16289-2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natalia Castaño-Rodríguez Khean-Lee Goh Kwong Ming Fock Hazel M. Mitchell Nadeem O. Kaakoush |
spellingShingle |
Natalia Castaño-Rodríguez Khean-Lee Goh Kwong Ming Fock Hazel M. Mitchell Nadeem O. Kaakoush Dysbiosis of the microbiome in gastric carcinogenesis Scientific Reports |
author_facet |
Natalia Castaño-Rodríguez Khean-Lee Goh Kwong Ming Fock Hazel M. Mitchell Nadeem O. Kaakoush |
author_sort |
Natalia Castaño-Rodríguez |
title |
Dysbiosis of the microbiome in gastric carcinogenesis |
title_short |
Dysbiosis of the microbiome in gastric carcinogenesis |
title_full |
Dysbiosis of the microbiome in gastric carcinogenesis |
title_fullStr |
Dysbiosis of the microbiome in gastric carcinogenesis |
title_full_unstemmed |
Dysbiosis of the microbiome in gastric carcinogenesis |
title_sort |
dysbiosis of the microbiome in gastric carcinogenesis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-11-01 |
description |
Abstract The gastric microbiome has been proposed as an etiological factor in gastric carcinogenesis. We compared the gastric microbiota in subjects presenting with gastric cancer (GC, n = 12) and controls (functional dyspepsia (FD), n = 20) from a high GC risk population in Singapore and Malaysia. cDNA from 16S rRNA transcripts were amplified (515F-806R) and sequenced using Illumina MiSeq 2 × 250 bp chemistry. Increased richness and phylogenetic diversity but not Shannon’s diversity was found in GC as compared to controls. nMDS clustered GC and FD subjects separately, with PERMANOVA confirming a significant difference between the groups. H. pylori serological status had a significant impact on gastric microbiome α-diversity and composition. Several bacterial taxa were enriched in GC, including Lactococcus, Veilonella, and Fusobacteriaceae (Fusobacterium and Leptotrichia). Prediction of bacterial metabolic contribution indicated that serological status had a significant impact on metabolic function, while carbohydrate digestion and pathways were enriched in GC. Our findings highlight three mechanisms of interest in GC, including enrichment of pro-inflammatory oral bacterial species, increased abundance of lactic acid producing bacteria, and enrichment of short chain fatty acid production pathways. |
url |
https://doi.org/10.1038/s41598-017-16289-2 |
work_keys_str_mv |
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