A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes
Due to the limited number of available antibiotics, antimicrobial peptides (AMPs) are considered antimicrobial candidates to fight difficult-to-treat infections such as those associated with biofilms. Marine environments are precious sources of AMPs, as shown by the recent discovery of antibiofilm p...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-03-01
|
Series: | Marine Drugs |
Subjects: | |
Online Access: | http://www.mdpi.com/1660-3397/17/3/159 |
id |
doaj-cf0d23d140c14f2db93520d7698e00d2 |
---|---|
record_format |
Article |
spelling |
doaj-cf0d23d140c14f2db93520d7698e00d22020-11-25T00:13:54ZengMDPI AGMarine Drugs1660-33972019-03-0117315910.3390/md17030159md17030159A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenesMaria Grazia Cusimano0Angelo Spinello1Giampaolo Barone2Domenico Schillaci3Stella Cascioferro4Alessandra Magistrato5Barbara Parrino6Vincenzo Arizza7Maria Vitale8Dipartimento di Scienze Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, ItalyCNR-IOM-Democritos c/o International School for Advanced Studies (SISSA), Via Bonomea 265, 34136 Trieste, ItalyDipartimento di Scienze Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, ItalyDipartimento di Scienze Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, ItalyDipartimento di Scienze Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, ItalyCNR-IOM-Democritos c/o International School for Advanced Studies (SISSA), Via Bonomea 265, 34136 Trieste, ItalyDipartimento di Scienze Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, ItalyDipartimento di Scienze Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, ItalyIstituto Zooprofilattico della Sicilia, Via Gino Marinuzzi, 3, 90129 Palermo, ItalyDue to the limited number of available antibiotics, antimicrobial peptides (AMPs) are considered antimicrobial candidates to fight difficult-to-treat infections such as those associated with biofilms. Marine environments are precious sources of AMPs, as shown by the recent discovery of antibiofilm properties of Holothuroidin 2 (H2), an AMP produced by the Mediterranean sea cucumber Holothuria tubulosa. In this study, we considered the properties of a new H2 derivative, named H2d, and we tested it against seven strains of the dangerous foodborne pathogen Listeria monocytogenes. This peptide was more active than H2 in inhibiting the growth of planktonic L. monocytogenes and was able to interfere with biofilm formation at sub-minimum inhibitory concentrations (MICs). Atomic-level molecular dynamics (MD) simulations revealed insights related to the enhanced inhibitory activity of H2d, showing that the peptide is characterized by a more defined tertiary structure with respect to its ancestor. This allows the peptide to better exhibit an amphipathic character, which is an essential requirement for the interaction with cell membranes, similarly to other AMPs. Altogether, these results support the potential use of our synthetic peptide, H2d, as a template for the development of novel AMP-based drugs able to fight foodborne that are resistant to conventional antibiotics.http://www.mdpi.com/1660-3397/17/3/159antimicrobial peptidesHolothuria tubulosafoodborne pathogensListeria monocytogenesbiofilm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Grazia Cusimano Angelo Spinello Giampaolo Barone Domenico Schillaci Stella Cascioferro Alessandra Magistrato Barbara Parrino Vincenzo Arizza Maria Vitale |
spellingShingle |
Maria Grazia Cusimano Angelo Spinello Giampaolo Barone Domenico Schillaci Stella Cascioferro Alessandra Magistrato Barbara Parrino Vincenzo Arizza Maria Vitale A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes Marine Drugs antimicrobial peptides Holothuria tubulosa foodborne pathogens Listeria monocytogenes biofilm |
author_facet |
Maria Grazia Cusimano Angelo Spinello Giampaolo Barone Domenico Schillaci Stella Cascioferro Alessandra Magistrato Barbara Parrino Vincenzo Arizza Maria Vitale |
author_sort |
Maria Grazia Cusimano |
title |
A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes |
title_short |
A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes |
title_full |
A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes |
title_fullStr |
A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes |
title_full_unstemmed |
A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes |
title_sort |
synthetic derivative of antimicrobial peptide holothuroidin 2 from mediterranean sea cucumber (holothuria tubulosa) in the control of listeria monocytogenes |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2019-03-01 |
description |
Due to the limited number of available antibiotics, antimicrobial peptides (AMPs) are considered antimicrobial candidates to fight difficult-to-treat infections such as those associated with biofilms. Marine environments are precious sources of AMPs, as shown by the recent discovery of antibiofilm properties of Holothuroidin 2 (H2), an AMP produced by the Mediterranean sea cucumber Holothuria tubulosa. In this study, we considered the properties of a new H2 derivative, named H2d, and we tested it against seven strains of the dangerous foodborne pathogen Listeria monocytogenes. This peptide was more active than H2 in inhibiting the growth of planktonic L. monocytogenes and was able to interfere with biofilm formation at sub-minimum inhibitory concentrations (MICs). Atomic-level molecular dynamics (MD) simulations revealed insights related to the enhanced inhibitory activity of H2d, showing that the peptide is characterized by a more defined tertiary structure with respect to its ancestor. This allows the peptide to better exhibit an amphipathic character, which is an essential requirement for the interaction with cell membranes, similarly to other AMPs. Altogether, these results support the potential use of our synthetic peptide, H2d, as a template for the development of novel AMP-based drugs able to fight foodborne that are resistant to conventional antibiotics. |
topic |
antimicrobial peptides Holothuria tubulosa foodborne pathogens Listeria monocytogenes biofilm |
url |
http://www.mdpi.com/1660-3397/17/3/159 |
work_keys_str_mv |
AT mariagraziacusimano asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT angelospinello asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT giampaolobarone asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT domenicoschillaci asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT stellacascioferro asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT alessandramagistrato asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT barbaraparrino asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT vincenzoarizza asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT mariavitale asyntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT mariagraziacusimano syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT angelospinello syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT giampaolobarone syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT domenicoschillaci syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT stellacascioferro syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT alessandramagistrato syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT barbaraparrino syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT vincenzoarizza syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes AT mariavitale syntheticderivativeofantimicrobialpeptideholothuroidin2frommediterraneanseacucumberholothuriatubulosainthecontroloflisteriamonocytogenes |
_version_ |
1725392465110761472 |