CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle
Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to decrease porcine subcutaneous (SC) adipose tissue levels while increasing the count of intramuscular (IM) adipose tissue in vivo. However, the underlying mechanisms through which it acts ar...
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doaj-cf13090a954c4357961b59f906227f652021-04-28T06:07:00ZengElsevierJournal of Lipid Research0022-22752007-08-0148817011709CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscleXuan Zhou0Defa Li1Jingdong Yin2Jianjun Ni3Bing Dong4Jinxiao Zhang5Min Du6National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, ChinaNational Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, ChinaNational Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, ChinaNational Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, ChinaNational Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, ChinaNational Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, ChinaDepartment of Animal Science, University of Wyoming, Laramie, WY 82071Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to decrease porcine subcutaneous (SC) adipose tissue levels while increasing the count of intramuscular (IM) adipose tissue in vivo. However, the underlying mechanisms through which it acts are poorly understood. The objective of this study was to investigate the different effects of CLA on adipogenesis in cultured SC adipose tissue and IM stromal vascular cells obtained from neonatal pigs. As shown here, trans-10, cis-12 CLA decreased the expression of adipocyte-specific genes as well as adipose precursor cell numbers and the accumulation of lipid in cultured SC adipose tissue stromal vascular cells. However, the cis-9, trans-11 CLA did not alter adipogenesis in SC cultures. On the other hand, both CLA isomers increased the expression of adipocyte-specific genes in IM cultures, together with the increasing accumulation of lipid and Oil Red O-stained cells. Collectively, these data show that CLA decreases SC adipose tissue but increases IM adipose tissue by different regulation of adipocyte-specific gene expression. These results suggest that adipogenesis in IM adipocytes differs from that in SC adipocytes.http://www.sciencedirect.com/science/article/pii/S0022227520425064adipocyte-specific geneadipose precursor cellproliferationdifferentiationsubcutaneous adipose tissueintramuscular adipose tissue |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xuan Zhou Defa Li Jingdong Yin Jianjun Ni Bing Dong Jinxiao Zhang Min Du |
spellingShingle |
Xuan Zhou Defa Li Jingdong Yin Jianjun Ni Bing Dong Jinxiao Zhang Min Du CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle Journal of Lipid Research adipocyte-specific gene adipose precursor cell proliferation differentiation subcutaneous adipose tissue intramuscular adipose tissue |
author_facet |
Xuan Zhou Defa Li Jingdong Yin Jianjun Ni Bing Dong Jinxiao Zhang Min Du |
author_sort |
Xuan Zhou |
title |
CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle |
title_short |
CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle |
title_full |
CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle |
title_fullStr |
CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle |
title_full_unstemmed |
CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle |
title_sort |
cla differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2007-08-01 |
description |
Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to decrease porcine subcutaneous (SC) adipose tissue levels while increasing the count of intramuscular (IM) adipose tissue in vivo. However, the underlying mechanisms through which it acts are poorly understood. The objective of this study was to investigate the different effects of CLA on adipogenesis in cultured SC adipose tissue and IM stromal vascular cells obtained from neonatal pigs. As shown here, trans-10, cis-12 CLA decreased the expression of adipocyte-specific genes as well as adipose precursor cell numbers and the accumulation of lipid in cultured SC adipose tissue stromal vascular cells. However, the cis-9, trans-11 CLA did not alter adipogenesis in SC cultures. On the other hand, both CLA isomers increased the expression of adipocyte-specific genes in IM cultures, together with the increasing accumulation of lipid and Oil Red O-stained cells. Collectively, these data show that CLA decreases SC adipose tissue but increases IM adipose tissue by different regulation of adipocyte-specific gene expression. These results suggest that adipogenesis in IM adipocytes differs from that in SC adipocytes. |
topic |
adipocyte-specific gene adipose precursor cell proliferation differentiation subcutaneous adipose tissue intramuscular adipose tissue |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520425064 |
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