Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas

Abstract Background Neck dissection is standard in surgical management of oral squamous cell carcinomas (oscc). However, the immunologic link between primary tumor and lymph nodes is insufficiently understood. Galectin 3 (Gal3) promotes M2 polarization of macrophages and contributes to immunosuppres...

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Main Authors: Falk Wehrhan, Maike Büttner-Herold, Luitpold Distel, Jutta Ries, Patrick Moebius, Raimund Preidl, Carol I. Geppert, Friedrich W. Neukam, Marco Kesting, Manuel Weber
Format: Article
Language:English
Published: BMC 2018-08-01
Series:BMC Cancer
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12885-018-4726-6
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spelling doaj-cf1b70f260f74382809c3624383aeab02020-11-24T21:24:39ZengBMCBMC Cancer1471-24072018-08-0118111010.1186/s12885-018-4726-6Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomasFalk Wehrhan0Maike Büttner-Herold1Luitpold Distel2Jutta Ries3Patrick Moebius4Raimund Preidl5Carol I. Geppert6Friedrich W. Neukam7Marco Kesting8Manuel Weber9Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-NürnbergDepartment of Nephropathology, Institute of Pathology, Friedrich-Alexander University Erlangen-NürnbergDepartment of Radiation Oncology, Friedrich-Alexander University Erlangen-NürnbergDepartment of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-NürnbergDepartment of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-NürnbergDepartment of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-NürnbergInstitute of Pathology, Friedrich-Alexander University Erlangen-NürnbergDepartment of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-NürnbergDepartment of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-NürnbergDepartment of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-NürnbergAbstract Background Neck dissection is standard in surgical management of oral squamous cell carcinomas (oscc). However, the immunologic link between primary tumor and lymph nodes is insufficiently understood. Galectin 3 (Gal3) promotes M2 polarization of macrophages and contributes to immunosuppression. The current study analyzes the association between Gal3 expression in regional lymph nodes of oscc with histomorphologic parameters (T-, N-, L- Pn-stage, grading) of the primary tumor. Additionally, Gal3 expression is correlated with markers of macrophage polarization (M1 vs. M2). Methods Preoperative diagnostic biopsies (n = 26), tumor resection specimens (n = 34), tumor-free lymph nodes (n = 28) and lymph node metastases (n = 10) of T1/T2 oscc patients were immunohistochemically analyzed for Gal3 and macrophage marker (CD68, CD11c, CD163 and MRC1) expression. The number of positive cells and the expression ratios were quantitatively assessed. Results High Gal3 expression in tumor-free regional lymph nodes was significantly (p < 0.05) associated with increased tumor size. The epithelial compartment of lymph node metastases showed a significantly (p < 0.05) increased Gal3 expression compared to biopsies and tumor resection specimens. Cell density of M2 macrophages was significantly (p < 0.05) and positively correlated with the number of Gal3 expressing cells in lymph nodes and tumor specimens. Conclusion Gal3 expression in regional lymph nodes might be associated with oscc progression. The increased Gal3 expression in regional lymph nodes of larger tumors underlines the need of immunomodulatory treatment concepts in early-stage oscc. Blocking of Gal3 might be a therapeutic option in oral cancer.http://link.springer.com/article/10.1186/s12885-018-4726-6Oral squamous cell carcinomaOsccLymph nodeMetastasisOral cancerGalectin 3
collection DOAJ
language English
format Article
sources DOAJ
author Falk Wehrhan
Maike Büttner-Herold
Luitpold Distel
Jutta Ries
Patrick Moebius
Raimund Preidl
Carol I. Geppert
Friedrich W. Neukam
Marco Kesting
Manuel Weber
spellingShingle Falk Wehrhan
Maike Büttner-Herold
Luitpold Distel
Jutta Ries
Patrick Moebius
Raimund Preidl
Carol I. Geppert
Friedrich W. Neukam
Marco Kesting
Manuel Weber
Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas
BMC Cancer
Oral squamous cell carcinoma
Oscc
Lymph node
Metastasis
Oral cancer
Galectin 3
author_facet Falk Wehrhan
Maike Büttner-Herold
Luitpold Distel
Jutta Ries
Patrick Moebius
Raimund Preidl
Carol I. Geppert
Friedrich W. Neukam
Marco Kesting
Manuel Weber
author_sort Falk Wehrhan
title Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas
title_short Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas
title_full Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas
title_fullStr Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas
title_full_unstemmed Galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas
title_sort galectin 3 expression in regional lymph nodes and lymph node metastases of oral squamous cell carcinomas
publisher BMC
series BMC Cancer
issn 1471-2407
publishDate 2018-08-01
description Abstract Background Neck dissection is standard in surgical management of oral squamous cell carcinomas (oscc). However, the immunologic link between primary tumor and lymph nodes is insufficiently understood. Galectin 3 (Gal3) promotes M2 polarization of macrophages and contributes to immunosuppression. The current study analyzes the association between Gal3 expression in regional lymph nodes of oscc with histomorphologic parameters (T-, N-, L- Pn-stage, grading) of the primary tumor. Additionally, Gal3 expression is correlated with markers of macrophage polarization (M1 vs. M2). Methods Preoperative diagnostic biopsies (n = 26), tumor resection specimens (n = 34), tumor-free lymph nodes (n = 28) and lymph node metastases (n = 10) of T1/T2 oscc patients were immunohistochemically analyzed for Gal3 and macrophage marker (CD68, CD11c, CD163 and MRC1) expression. The number of positive cells and the expression ratios were quantitatively assessed. Results High Gal3 expression in tumor-free regional lymph nodes was significantly (p < 0.05) associated with increased tumor size. The epithelial compartment of lymph node metastases showed a significantly (p < 0.05) increased Gal3 expression compared to biopsies and tumor resection specimens. Cell density of M2 macrophages was significantly (p < 0.05) and positively correlated with the number of Gal3 expressing cells in lymph nodes and tumor specimens. Conclusion Gal3 expression in regional lymph nodes might be associated with oscc progression. The increased Gal3 expression in regional lymph nodes of larger tumors underlines the need of immunomodulatory treatment concepts in early-stage oscc. Blocking of Gal3 might be a therapeutic option in oral cancer.
topic Oral squamous cell carcinoma
Oscc
Lymph node
Metastasis
Oral cancer
Galectin 3
url http://link.springer.com/article/10.1186/s12885-018-4726-6
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