Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells
In this study, a transferrin (T<sub>f</sub>)-conjugated polymeric nanoparticle was developed for the targeted delivery of the chemotherapeutic agent doxorubicin (Dox) in order to overcome multi-drug resistance in cancer treatment. Our objective was to improve Dox delivery for producing s...
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doaj-cf38fda9d6084500b7db97c43e006c6f2020-11-25T02:16:02ZengMDPI AGPharmaceutics1999-49232019-02-011126310.3390/pharmaceutics11020063pharmaceutics11020063Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer CellsZar Chi Soe0Jun Bum Kwon1Raj Kumar Thapa2Wenquan Ou3Hanh Thuy Nguyen4Milan Gautam5Kyung Taek Oh6Han-Gon Choi7Sae Kwang Ku8Chul Soon Yong9Jong Oh Kim10College of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaCollege of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaCollege of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaCollege of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaCollege of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaCollege of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaCollege of Pharmacy, Chung-Ang University, 221 Heuksuk-dong Dongjak-gu, Seoul 156-756, KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, KoreaCollege of Korean Medicine, Daegu Haany University, Gyeongsan 712-715, KoreaCollege of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaCollege of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, KoreaIn this study, a transferrin (T<sub>f</sub>)-conjugated polymeric nanoparticle was developed for the targeted delivery of the chemotherapeutic agent doxorubicin (Dox) in order to overcome multi-drug resistance in cancer treatment. Our objective was to improve Dox delivery for producing significant antitumor efficacy in Dox-resistant (R) breast cancer cell lines with minimum toxicity to healthy cells. The results of our experiments revealed that Dox was successfully loaded inside a transferrin (T<sub>f</sub>)-conjugated polymeric nanoparticle composed of poloxamer 407 (F127) and 123 (P123) (Dox/F127<i>&</i>P123-T<sub>f</sub>), which produced nanosized particles (~90 nm) with a low polydispersity index (~0.23). The accelerated and controlled release profiles of Dox from the nanoparticles were characterized in acidic and physiological pH and Dox/F127<i>&</i>P123-T<sub>f</sub> enhanced Dox cytotoxicity in OVCAR-3, MDA-MB-231, and MDA-MB-231(R) cell lines through induction of cellular apoptosis. Moreover, Dox/F127<i>&</i>P123-T<sub>f</sub> inhibited cell migration and altered the cell cycle patterns of different cancer cells. In vivo study in MDA-MB-231(R) tumor-bearing mice demonstrated enhanced delivery of nanoparticles to the tumor site when coated in a targeting moiety. Therefore, Dox/F127<i>&</i>P123-T<sub>f</sub> has been tailored, using the principles of nanotherapeutics, to overcome drug-resistant chemotherapy.https://www.mdpi.com/1999-4923/11/2/63doxorubicindoxorubicin-resistant cancerpolymeric nanoparticlestransferrin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zar Chi Soe Jun Bum Kwon Raj Kumar Thapa Wenquan Ou Hanh Thuy Nguyen Milan Gautam Kyung Taek Oh Han-Gon Choi Sae Kwang Ku Chul Soon Yong Jong Oh Kim |
spellingShingle |
Zar Chi Soe Jun Bum Kwon Raj Kumar Thapa Wenquan Ou Hanh Thuy Nguyen Milan Gautam Kyung Taek Oh Han-Gon Choi Sae Kwang Ku Chul Soon Yong Jong Oh Kim Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells Pharmaceutics doxorubicin doxorubicin-resistant cancer polymeric nanoparticles transferrin |
author_facet |
Zar Chi Soe Jun Bum Kwon Raj Kumar Thapa Wenquan Ou Hanh Thuy Nguyen Milan Gautam Kyung Taek Oh Han-Gon Choi Sae Kwang Ku Chul Soon Yong Jong Oh Kim |
author_sort |
Zar Chi Soe |
title |
Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells |
title_short |
Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells |
title_full |
Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells |
title_fullStr |
Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells |
title_full_unstemmed |
Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells |
title_sort |
transferrin-conjugated polymeric nanoparticle for receptor-mediated delivery of doxorubicin in doxorubicin-resistant breast cancer cells |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2019-02-01 |
description |
In this study, a transferrin (T<sub>f</sub>)-conjugated polymeric nanoparticle was developed for the targeted delivery of the chemotherapeutic agent doxorubicin (Dox) in order to overcome multi-drug resistance in cancer treatment. Our objective was to improve Dox delivery for producing significant antitumor efficacy in Dox-resistant (R) breast cancer cell lines with minimum toxicity to healthy cells. The results of our experiments revealed that Dox was successfully loaded inside a transferrin (T<sub>f</sub>)-conjugated polymeric nanoparticle composed of poloxamer 407 (F127) and 123 (P123) (Dox/F127<i>&</i>P123-T<sub>f</sub>), which produced nanosized particles (~90 nm) with a low polydispersity index (~0.23). The accelerated and controlled release profiles of Dox from the nanoparticles were characterized in acidic and physiological pH and Dox/F127<i>&</i>P123-T<sub>f</sub> enhanced Dox cytotoxicity in OVCAR-3, MDA-MB-231, and MDA-MB-231(R) cell lines through induction of cellular apoptosis. Moreover, Dox/F127<i>&</i>P123-T<sub>f</sub> inhibited cell migration and altered the cell cycle patterns of different cancer cells. In vivo study in MDA-MB-231(R) tumor-bearing mice demonstrated enhanced delivery of nanoparticles to the tumor site when coated in a targeting moiety. Therefore, Dox/F127<i>&</i>P123-T<sub>f</sub> has been tailored, using the principles of nanotherapeutics, to overcome drug-resistant chemotherapy. |
topic |
doxorubicin doxorubicin-resistant cancer polymeric nanoparticles transferrin |
url |
https://www.mdpi.com/1999-4923/11/2/63 |
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