Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication
<p>Abstract</p> <p>Background</p> <p>An understanding of host cell factors that affect viral replication contributes to elucidation of the mechanism for determination of viral tropism. Cyclophilin A (CypA), a peptidyl-prolyl <it>cis-trans </it>isomerase (PPI...
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doaj-cf5217f72a2943a4bf73b0b21ae8b5b72020-11-25T00:37:55ZengBMCRetrovirology1742-46902012-01-0191310.1186/1742-4690-9-3Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replicationTakeuchi HiroakiIshii HiroshiKuwano TetsuyaInagaki NatsukoAkari HirofumiMatano Tetsuro<p>Abstract</p> <p>Background</p> <p>An understanding of host cell factors that affect viral replication contributes to elucidation of the mechanism for determination of viral tropism. Cyclophilin A (CypA), a peptidyl-prolyl <it>cis-trans </it>isomerase (PPIase), is a host factor essential for efficient replication of human immunodeficiency virus type 1 (HIV-1) in human cells. However, the role of cyclophilins in simian immunodeficiency virus (SIV) replication has not been determined. In the present study, we examined the effect of cyclosporine A (CsA), a PPIase inhibitor, on SIV replication.</p> <p>Results</p> <p>SIV replication in human CEM-SS T cells was not inhibited but rather enhanced by treatment with CsA, which inhibited HIV-1 replication. CsA treatment of target human cells enhanced an early step of SIV replication. CypA overexpression enhanced the early phase of HIV-1 but not SIV replication, while CypA knock-down resulted in suppression of HIV-1 but not SIV replication in CEM-SS cells, partially explaining different sensitivities of HIV-1 and SIV replication to CsA treatment. In contrast, CsA treatment inhibited SIV replication in macaque T cells; CsA treatment of either virus producer or target cells resulted in suppression of SIV replication. SIV infection was enhanced by CypA overexpression in macaque target cells.</p> <p>Conclusions</p> <p>CsA treatment enhanced SIV replication in human T cells but abrogated SIV replication in macaque T cells, implying a host cell species-specific effect of CsA on SIV replication. Further analyses indicated a positive effect of CypA on SIV infection into macaque but not into human T cells. These results suggest possible contribution of CypA to the determination of SIV tropism.</p> http://www.retrovirology.com/content/9/1/3HIV-1SIVcyclophilin Acyclophilin Bcyclosporine Atropism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takeuchi Hiroaki Ishii Hiroshi Kuwano Tetsuya Inagaki Natsuko Akari Hirofumi Matano Tetsuro |
spellingShingle |
Takeuchi Hiroaki Ishii Hiroshi Kuwano Tetsuya Inagaki Natsuko Akari Hirofumi Matano Tetsuro Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication Retrovirology HIV-1 SIV cyclophilin A cyclophilin B cyclosporine A tropism |
author_facet |
Takeuchi Hiroaki Ishii Hiroshi Kuwano Tetsuya Inagaki Natsuko Akari Hirofumi Matano Tetsuro |
author_sort |
Takeuchi Hiroaki |
title |
Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication |
title_short |
Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication |
title_full |
Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication |
title_fullStr |
Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication |
title_full_unstemmed |
Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication |
title_sort |
host cell species-specific effect of cyclosporine a on simian immunodeficiency virus replication |
publisher |
BMC |
series |
Retrovirology |
issn |
1742-4690 |
publishDate |
2012-01-01 |
description |
<p>Abstract</p> <p>Background</p> <p>An understanding of host cell factors that affect viral replication contributes to elucidation of the mechanism for determination of viral tropism. Cyclophilin A (CypA), a peptidyl-prolyl <it>cis-trans </it>isomerase (PPIase), is a host factor essential for efficient replication of human immunodeficiency virus type 1 (HIV-1) in human cells. However, the role of cyclophilins in simian immunodeficiency virus (SIV) replication has not been determined. In the present study, we examined the effect of cyclosporine A (CsA), a PPIase inhibitor, on SIV replication.</p> <p>Results</p> <p>SIV replication in human CEM-SS T cells was not inhibited but rather enhanced by treatment with CsA, which inhibited HIV-1 replication. CsA treatment of target human cells enhanced an early step of SIV replication. CypA overexpression enhanced the early phase of HIV-1 but not SIV replication, while CypA knock-down resulted in suppression of HIV-1 but not SIV replication in CEM-SS cells, partially explaining different sensitivities of HIV-1 and SIV replication to CsA treatment. In contrast, CsA treatment inhibited SIV replication in macaque T cells; CsA treatment of either virus producer or target cells resulted in suppression of SIV replication. SIV infection was enhanced by CypA overexpression in macaque target cells.</p> <p>Conclusions</p> <p>CsA treatment enhanced SIV replication in human T cells but abrogated SIV replication in macaque T cells, implying a host cell species-specific effect of CsA on SIV replication. Further analyses indicated a positive effect of CypA on SIV infection into macaque but not into human T cells. These results suggest possible contribution of CypA to the determination of SIV tropism.</p> |
topic |
HIV-1 SIV cyclophilin A cyclophilin B cyclosporine A tropism |
url |
http://www.retrovirology.com/content/9/1/3 |
work_keys_str_mv |
AT takeuchihiroaki hostcellspeciesspecificeffectofcyclosporineaonsimianimmunodeficiencyvirusreplication AT ishiihiroshi hostcellspeciesspecificeffectofcyclosporineaonsimianimmunodeficiencyvirusreplication AT kuwanotetsuya hostcellspeciesspecificeffectofcyclosporineaonsimianimmunodeficiencyvirusreplication AT inagakinatsuko hostcellspeciesspecificeffectofcyclosporineaonsimianimmunodeficiencyvirusreplication AT akarihirofumi hostcellspeciesspecificeffectofcyclosporineaonsimianimmunodeficiencyvirusreplication AT matanotetsuro hostcellspeciesspecificeffectofcyclosporineaonsimianimmunodeficiencyvirusreplication |
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