FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels
Fibrin hydrogel is a central biological material in tissue engineering and drug delivery applications. As such, fibrin is typically combined with cells and biomolecules targeted to the regenerated tissue. Previous studies have analyzed the release of different molecules from fibrin hydrogels; howeve...
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doaj-cf71ae1ffa7e444cb8fc9978137b2cf32021-02-24T00:05:37ZengMDPI AGBiomolecules2218-273X2021-02-011133733710.3390/biom11020337FITC-Dextran Release from Cell-Embedded Fibrin HydrogelsViki Raz Lepsky0Sari Natan1Oren Tchaicheeyan2Avraham Kolel3Merav Zussman4Meital Zilberman5Ayelet Lesman6Department of Materials Science and Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, IsraelSchool of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, IsraelSchool of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, IsraelDepartment of Biomedical Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, IsraelDepartment of Materials Science and Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, IsraelDepartment of Materials Science and Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, IsraelSchool of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, IsraelFibrin hydrogel is a central biological material in tissue engineering and drug delivery applications. As such, fibrin is typically combined with cells and biomolecules targeted to the regenerated tissue. Previous studies have analyzed the release of different molecules from fibrin hydrogels; however, the effect of embedded cells on the release profile has yet to be quantitatively explored. This study focused on the release of Fluorescein isothiocyanate (FITC)-dextran (FD) 250 kDa from fibrin hydrogels, populated with different concentrations of fibroblast or endothelial cells, during a 48-h observation period. The addition of cells to fibrin gels decreased the overall release by a small percentage (by 7–15% for fibroblasts and 6–8% for endothelial cells) relative to acellular gels. The release profile was shown to be modulated by various cellular activities, including gel degradation and physical obstruction to diffusion. Cell-generated forces and matrix deformation (i.e., densification and fiber alignment) were not found to significantly influence the release profiles. This knowledge is expected to improve fibrin integration in tissue engineering and drug delivery applications by enabling predictions and ways to modulate the release profiles of various biomolecules.https://www.mdpi.com/2218-273X/11/2/337drug deliverycontrolled releasehydrogelsfibrinFITC-dextran (FD)tissue engineering |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Viki Raz Lepsky Sari Natan Oren Tchaicheeyan Avraham Kolel Merav Zussman Meital Zilberman Ayelet Lesman |
spellingShingle |
Viki Raz Lepsky Sari Natan Oren Tchaicheeyan Avraham Kolel Merav Zussman Meital Zilberman Ayelet Lesman FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels Biomolecules drug delivery controlled release hydrogels fibrin FITC-dextran (FD) tissue engineering |
author_facet |
Viki Raz Lepsky Sari Natan Oren Tchaicheeyan Avraham Kolel Merav Zussman Meital Zilberman Ayelet Lesman |
author_sort |
Viki Raz Lepsky |
title |
FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels |
title_short |
FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels |
title_full |
FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels |
title_fullStr |
FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels |
title_full_unstemmed |
FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels |
title_sort |
fitc-dextran release from cell-embedded fibrin hydrogels |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2021-02-01 |
description |
Fibrin hydrogel is a central biological material in tissue engineering and drug delivery applications. As such, fibrin is typically combined with cells and biomolecules targeted to the regenerated tissue. Previous studies have analyzed the release of different molecules from fibrin hydrogels; however, the effect of embedded cells on the release profile has yet to be quantitatively explored. This study focused on the release of Fluorescein isothiocyanate (FITC)-dextran (FD) 250 kDa from fibrin hydrogels, populated with different concentrations of fibroblast or endothelial cells, during a 48-h observation period. The addition of cells to fibrin gels decreased the overall release by a small percentage (by 7–15% for fibroblasts and 6–8% for endothelial cells) relative to acellular gels. The release profile was shown to be modulated by various cellular activities, including gel degradation and physical obstruction to diffusion. Cell-generated forces and matrix deformation (i.e., densification and fiber alignment) were not found to significantly influence the release profiles. This knowledge is expected to improve fibrin integration in tissue engineering and drug delivery applications by enabling predictions and ways to modulate the release profiles of various biomolecules. |
topic |
drug delivery controlled release hydrogels fibrin FITC-dextran (FD) tissue engineering |
url |
https://www.mdpi.com/2218-273X/11/2/337 |
work_keys_str_mv |
AT vikirazlepsky fitcdextranreleasefromcellembeddedfibrinhydrogels AT sarinatan fitcdextranreleasefromcellembeddedfibrinhydrogels AT orentchaicheeyan fitcdextranreleasefromcellembeddedfibrinhydrogels AT avrahamkolel fitcdextranreleasefromcellembeddedfibrinhydrogels AT meravzussman fitcdextranreleasefromcellembeddedfibrinhydrogels AT meitalzilberman fitcdextranreleasefromcellembeddedfibrinhydrogels AT ayeletlesman fitcdextranreleasefromcellembeddedfibrinhydrogels |
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