Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing

Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing

This Data in Brief article is a supporting information for the research article entitled “Protistan community composition in anoxic sediments from three salinity-disparate Japanese lakes” by Kataoka and Kondo (2019) [1]. Summary of 18S rRNA gene sequences originated from anoxic sediment of three lak...

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Main Authors: Takafumi Kataoka, Ryuji Kondo
Format: Article
Language:English
Published: Elsevier 2019-08-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340919305670
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spelling doaj-cf8ee2036d004790bf38017342274b832020-11-25T02:33:15ZengElsevierData in Brief2352-34092019-08-0125Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencingTakafumi Kataoka0Ryuji Kondo1Department of Marine Science and Technology, Fukui Prefectural University, Obama, Fukui, 917-0003, JapanCorresponding author.; Department of Marine Science and Technology, Fukui Prefectural University, Obama, Fukui, 917-0003, JapanThis Data in Brief article is a supporting information for the research article entitled “Protistan community composition in anoxic sediments from three salinity-disparate Japanese lakes” by Kataoka and Kondo (2019) [1]. Summary of 18S rRNA gene sequences originated from anoxic sediment of three lakes in two seasons using high throughput sequencing techniques (MiSeq, Illumina) was shown in this data article. Supergroup-level taxonomy was compared between the SILVA search for SILVA database and BLASTn search for the PR2 database. Alpha diversity was calculated in each sample, and beta-diversity was calculated among the six amplicon libraries. Partial sequence length between the primer set of 574*f and 1132R Hugerth et al., 2015 was compared between the forward read and the combined read. Keywords: Protists, 18S rRNA gene, High throughput sequencing (HTS), MiSeq, V4–V5 hypervariable regionhttp://www.sciencedirect.com/science/article/pii/S2352340919305670
collection DOAJ
language English
format Article
sources DOAJ
author Takafumi Kataoka
Ryuji Kondo
spellingShingle Takafumi Kataoka
Ryuji Kondo
Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing
Data in Brief
author_facet Takafumi Kataoka
Ryuji Kondo
author_sort Takafumi Kataoka
title Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing
title_short Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing
title_full Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing
title_fullStr Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing
title_full_unstemmed Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing
title_sort data on taxonomic annotation and diversity of 18s rrna gene amplicon libraries derived from high throughput sequencing
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2019-08-01
description This Data in Brief article is a supporting information for the research article entitled “Protistan community composition in anoxic sediments from three salinity-disparate Japanese lakes” by Kataoka and Kondo (2019) [1]. Summary of 18S rRNA gene sequences originated from anoxic sediment of three lakes in two seasons using high throughput sequencing techniques (MiSeq, Illumina) was shown in this data article. Supergroup-level taxonomy was compared between the SILVA search for SILVA database and BLASTn search for the PR2 database. Alpha diversity was calculated in each sample, and beta-diversity was calculated among the six amplicon libraries. Partial sequence length between the primer set of 574*f and 1132R Hugerth et al., 2015 was compared between the forward read and the combined read. Keywords: Protists, 18S rRNA gene, High throughput sequencing (HTS), MiSeq, V4–V5 hypervariable region
url http://www.sciencedirect.com/science/article/pii/S2352340919305670
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AT ryujikondo dataontaxonomicannotationanddiversityof18srrnageneampliconlibrariesderivedfromhighthroughputsequencing
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