Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms
Tick-borne pathogens (TBPs), especially Anaplasma phagocytophilum, cause disease in grazing livestock. Tick prophylaxis is, therefore, a routine practice in sheep flocks in Sweden, especially in central, southern, and coastal areas of the country where ixodid ticks (Ixodes ricinus and Haemaphysalis...
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doaj-cfb1621ce7ee470685448ba7568e60a92020-11-24T23:47:58ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692018-04-01510.3389/fvets.2018.00072341875Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish FarmsGiulio Grandi0Giulio Grandi1Anna Aspán2Jenny Pihl3Katarina Gustafsson4Fredrik Engström5Tomas Jinnerot6Robert Söderlund7Jan Chirico8Department of Microbiology, National Veterinary Institute (SVA), Uppsala, SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Section for Parasitology, Swedish University of Agricultural Sciences (SLU), Uppsala, SwedenDepartment of Microbiology, National Veterinary Institute (SVA), Uppsala, SwedenDepartment of Microbiology, National Veterinary Institute (SVA), Uppsala, SwedenFarm and Animal Health, Uppsala, SwedenFarm and Animal Health, Uppsala, SwedenDepartment of Microbiology, National Veterinary Institute (SVA), Uppsala, SwedenDepartment of Microbiology, National Veterinary Institute (SVA), Uppsala, SwedenDepartment of Microbiology, National Veterinary Institute (SVA), Uppsala, SwedenTick-borne pathogens (TBPs), especially Anaplasma phagocytophilum, cause disease in grazing livestock. Tick prophylaxis is, therefore, a routine practice in sheep flocks in Sweden, especially in central, southern, and coastal areas of the country where ixodid ticks (Ixodes ricinus and Haemaphysalis punctata) are present. In the present study, the status of infection by A. phagocytophilum and other TBPs in lambs treated with tick prophylaxis has been assessed serologically and with polymerase chain reaction (PCR). Blood samples (n = 78) from lambs (n = 20) subjected to regular tick prophylactic treatment (flumethrin, Bayticol®) at two sites in different regions in Sweden (Östergötland, Gotland) were collected on four occasions from May until July 2013. The severity of clinical signs in Anaplasma-infected animals is known to differ between these two regions. In total, 20% of blood samples were PCR-positive for A. phagocytophilum. Serological analyses showed that 33% of all collected samples were positive for A. phagocytophilum, while 2.5% were positive for Borrelia burgdorferi s.l. and 13% for tick-borne encephalitis virus (TBEV). Percentages of lambs positive were 75 and 45% for A. phagocytophilum antibodies and DNA, respectively, while 10 and 45% were serologically positive for B. burgdorferi s.l. and TBEV, respectively. Sequencing of partial 16S rRNA genes from Anaplasma PCR positive samples revealed presence of A. phagocytophilum in all animals in Östergötland, while sequences consistent with A. phagocytophilum as well as A. capra and A. bovis were found on the island of Gotland. This is the first report of the occurrence of the latter two species in Sweden.http://journal.frontiersin.org/article/10.3389/fvets.2018.00072/fulltick prophylaxistick-borne pathogenssheepserologypolymerase chain reaction16S rRNA sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giulio Grandi Giulio Grandi Anna Aspán Jenny Pihl Katarina Gustafsson Fredrik Engström Tomas Jinnerot Robert Söderlund Jan Chirico |
spellingShingle |
Giulio Grandi Giulio Grandi Anna Aspán Jenny Pihl Katarina Gustafsson Fredrik Engström Tomas Jinnerot Robert Söderlund Jan Chirico Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms Frontiers in Veterinary Science tick prophylaxis tick-borne pathogens sheep serology polymerase chain reaction 16S rRNA sequencing |
author_facet |
Giulio Grandi Giulio Grandi Anna Aspán Jenny Pihl Katarina Gustafsson Fredrik Engström Tomas Jinnerot Robert Söderlund Jan Chirico |
author_sort |
Giulio Grandi |
title |
Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms |
title_short |
Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms |
title_full |
Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms |
title_fullStr |
Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms |
title_full_unstemmed |
Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms |
title_sort |
detection of tick-borne pathogens in lambs undergoing prophylactic treatment against ticks on two swedish farms |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2018-04-01 |
description |
Tick-borne pathogens (TBPs), especially Anaplasma phagocytophilum, cause disease in grazing livestock. Tick prophylaxis is, therefore, a routine practice in sheep flocks in Sweden, especially in central, southern, and coastal areas of the country where ixodid ticks (Ixodes ricinus and Haemaphysalis punctata) are present. In the present study, the status of infection by A. phagocytophilum and other TBPs in lambs treated with tick prophylaxis has been assessed serologically and with polymerase chain reaction (PCR). Blood samples (n = 78) from lambs (n = 20) subjected to regular tick prophylactic treatment (flumethrin, Bayticol®) at two sites in different regions in Sweden (Östergötland, Gotland) were collected on four occasions from May until July 2013. The severity of clinical signs in Anaplasma-infected animals is known to differ between these two regions. In total, 20% of blood samples were PCR-positive for A. phagocytophilum. Serological analyses showed that 33% of all collected samples were positive for A. phagocytophilum, while 2.5% were positive for Borrelia burgdorferi s.l. and 13% for tick-borne encephalitis virus (TBEV). Percentages of lambs positive were 75 and 45% for A. phagocytophilum antibodies and DNA, respectively, while 10 and 45% were serologically positive for B. burgdorferi s.l. and TBEV, respectively. Sequencing of partial 16S rRNA genes from Anaplasma PCR positive samples revealed presence of A. phagocytophilum in all animals in Östergötland, while sequences consistent with A. phagocytophilum as well as A. capra and A. bovis were found on the island of Gotland. This is the first report of the occurrence of the latter two species in Sweden. |
topic |
tick prophylaxis tick-borne pathogens sheep serology polymerase chain reaction 16S rRNA sequencing |
url |
http://journal.frontiersin.org/article/10.3389/fvets.2018.00072/full |
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