Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice
Leaf shape is an important agronomic trait for constructing an ideal plant type in rice, and high-density genetic map is facilitative in improving accuracy and efficiency for quantitative trait loci (QTL) analysis of leaf trait. In this study, a high-density genetic map contained 10,760 specific len...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.01143/full |
| id |
doaj-cfb1be2b2eae40a1b98045cb80306ded |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yi Wen Yi Wen Yunxia Fang Peng Hu Yiqing Tan Yueying Wang Linlin Hou Xuemei Deng Hao Wu Lixin Zhu Li Zhu Guang Chen Dali Zeng Longbiao Guo Guangheng Zhang Zhenyu Gao Guojun Dong Deyong Ren Lan Shen Qiang Zhang Dawei Xue Qian Qian Qian Qian Jiang Hu |
| spellingShingle |
Yi Wen Yi Wen Yunxia Fang Peng Hu Yiqing Tan Yueying Wang Linlin Hou Xuemei Deng Hao Wu Lixin Zhu Li Zhu Guang Chen Dali Zeng Longbiao Guo Guangheng Zhang Zhenyu Gao Guojun Dong Deyong Ren Lan Shen Qiang Zhang Dawei Xue Qian Qian Qian Qian Jiang Hu Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice Frontiers in Plant Science rice leaf quantitative trait loci genetic map specific length amplified fragment sequencing fine mapping |
| author_facet |
Yi Wen Yi Wen Yunxia Fang Peng Hu Yiqing Tan Yueying Wang Linlin Hou Xuemei Deng Hao Wu Lixin Zhu Li Zhu Guang Chen Dali Zeng Longbiao Guo Guangheng Zhang Zhenyu Gao Guojun Dong Deyong Ren Lan Shen Qiang Zhang Dawei Xue Qian Qian Qian Qian Jiang Hu |
| author_sort |
Yi Wen |
| title |
Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice |
| title_short |
Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice |
| title_full |
Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice |
| title_fullStr |
Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice |
| title_full_unstemmed |
Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice |
| title_sort |
construction of a high-density genetic map based on slaf markers and qtl analysis of leaf size in rice |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Plant Science |
| issn |
1664-462X |
| publishDate |
2020-07-01 |
| description |
Leaf shape is an important agronomic trait for constructing an ideal plant type in rice, and high-density genetic map is facilitative in improving accuracy and efficiency for quantitative trait loci (QTL) analysis of leaf trait. In this study, a high-density genetic map contained 10,760 specific length amplified fragment sequencing (SLAF) markers was established based on 149 recombinant inbred lines (RILs) derived from the cross between Rekuangeng (RKG) and Taizhong1 (TN1), which exhibited 1,613.59 cM map distance with an average interval of 0.17 cM. A total of 24 QTLs were detected and explained the phenotypic variance ranged from 9% to 33.8% related to the leaf morphology across two areas. Among them, one uncloned major QTL qTLLW1 (qTLL1 and qTLLW1) involved in regulating leaf length and leaf width with max 33.8% and 22.5% phenotypic variance respectively was located on chromosome 1, and another major locus qTLW4 affecting leaf width accounted for max 25.3% phenotypic variance was mapped on chromosome 4. Fine mapping and qRT-PCR expression analysis indicated that qTLW4 may be allelic to NAL1 (Narrow leaf 1) gene. |
| topic |
rice leaf quantitative trait loci genetic map specific length amplified fragment sequencing fine mapping |
| url |
https://www.frontiersin.org/article/10.3389/fpls.2020.01143/full |
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1724557700841340928 |
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doaj-cfb1be2b2eae40a1b98045cb80306ded2020-11-25T03:34:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-07-011110.3389/fpls.2020.01143560916Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in RiceYi Wen0Yi Wen1Yunxia Fang2Peng Hu3Yiqing Tan4Yueying Wang5Linlin Hou6Xuemei Deng7Hao Wu8Lixin Zhu9Li Zhu10Guang Chen11Dali Zeng12Longbiao Guo13Guangheng Zhang14Zhenyu Gao15Guojun Dong16Deyong Ren17Lan Shen18Qiang Zhang19Dawei Xue20Qian Qian21Qian Qian22Jiang Hu23State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaRice Research Institute of Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education and Liaoning Province, Shenyang, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaRice Research Institute of Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education and Liaoning Province, Shenyang, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaLeaf shape is an important agronomic trait for constructing an ideal plant type in rice, and high-density genetic map is facilitative in improving accuracy and efficiency for quantitative trait loci (QTL) analysis of leaf trait. In this study, a high-density genetic map contained 10,760 specific length amplified fragment sequencing (SLAF) markers was established based on 149 recombinant inbred lines (RILs) derived from the cross between Rekuangeng (RKG) and Taizhong1 (TN1), which exhibited 1,613.59 cM map distance with an average interval of 0.17 cM. A total of 24 QTLs were detected and explained the phenotypic variance ranged from 9% to 33.8% related to the leaf morphology across two areas. Among them, one uncloned major QTL qTLLW1 (qTLL1 and qTLLW1) involved in regulating leaf length and leaf width with max 33.8% and 22.5% phenotypic variance respectively was located on chromosome 1, and another major locus qTLW4 affecting leaf width accounted for max 25.3% phenotypic variance was mapped on chromosome 4. Fine mapping and qRT-PCR expression analysis indicated that qTLW4 may be allelic to NAL1 (Narrow leaf 1) gene.https://www.frontiersin.org/article/10.3389/fpls.2020.01143/fullriceleafquantitative trait locigenetic mapspecific length amplified fragment sequencingfine mapping |