Eco-friendly management of enteroviruses in wastewater
The wastewater treatment facility in El-Manzala (sedimentation basins followed by vegetated cells) was investigated to evaluate its performance as a low cost treatment facility in managing viral pollution. Sedimentation basins are considered to be physical treatment, while vegetated cells are consid...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2013-10-01
|
Series: | Water Science |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1110492913000039 |
id |
doaj-cfb40833a90b49db90139943cd33c0a2 |
---|---|
record_format |
Article |
spelling |
doaj-cfb40833a90b49db90139943cd33c0a22021-03-02T06:39:07ZengTaylor & Francis GroupWater Science1110-49292013-10-012754192910.1016/j.wsj.2013.12.002Eco-friendly management of enteroviruses in wastewaterAbeer M. Hegazy0Azza H. El-Salakawy1Mohamed M. Shaban2Mohamed M. Yehia3Mohamed S. AbuSalama4National Water Research Center (NWRC), Central Laboratories for Environmental Quality Monitoring (CLEQM), EgyptMicrobiology Department, Faculty of Medicine, Al-Azhar University, EgyptInstitute of Environmental Studies & Research, Ain Shams University, EgyptNational Water Research Center (NWRC), Central Laboratories for Environmental Quality Monitoring (CLEQM), EgyptNational Water Research Center (NWRC), Drainage Research Institute (DRI), EgyptThe wastewater treatment facility in El-Manzala (sedimentation basins followed by vegetated cells) was investigated to evaluate its performance as a low cost treatment facility in managing viral pollution. Sedimentation basins are considered to be physical treatment, while vegetated cells are considered as biological treatment. Enteroviruses were detected and determined using two techniques, cell culture as infectivity assay and real time reverse transcriptase-polymerase chain reaction (real time RT-PCR) as a molecular biological technology. The results revealed that the wastewater treatment facility in El-Manzala showed good performance under Egyptian climatic conditions. The aquatic macrophytes system (biological treatment) showed better performance compared to the sedimentation basins (physical treatment) in removing viral loads. Enteroviruses load at the wetland inlet ranged between 50 and 100% as viral infectivity (VI) by cell culture, and between 4.9 × 104 and 59.5 × 105 gene copies (GC) by real time RT-PCR. The virus load after sedimentation basins ranged between 25 and 50% as VI and between 3.7 × 102 and 4.5 × 104 GC, while after the biological treatment recorded 0% as VI and between 0 and 2 GC. An empirical model was developed to describe the relationship between the quantity of enteroviruses using molecular biology and infective assay as a predictor variable. The present study concluded that the wastewater treatment wetland in El-Manzala can be considered as an effective facility in reducing viral contamination of the Bahr El-Baqar drainage water.http://www.sciencedirect.com/science/article/pii/S1110492913000039Constructed wetlandPhysical treatmentBiological treatmentWater pollutionEnterovirusesPCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abeer M. Hegazy Azza H. El-Salakawy Mohamed M. Shaban Mohamed M. Yehia Mohamed S. AbuSalama |
spellingShingle |
Abeer M. Hegazy Azza H. El-Salakawy Mohamed M. Shaban Mohamed M. Yehia Mohamed S. AbuSalama Eco-friendly management of enteroviruses in wastewater Water Science Constructed wetland Physical treatment Biological treatment Water pollution Enteroviruses PCR |
author_facet |
Abeer M. Hegazy Azza H. El-Salakawy Mohamed M. Shaban Mohamed M. Yehia Mohamed S. AbuSalama |
author_sort |
Abeer M. Hegazy |
title |
Eco-friendly management of enteroviruses in wastewater |
title_short |
Eco-friendly management of enteroviruses in wastewater |
title_full |
Eco-friendly management of enteroviruses in wastewater |
title_fullStr |
Eco-friendly management of enteroviruses in wastewater |
title_full_unstemmed |
Eco-friendly management of enteroviruses in wastewater |
title_sort |
eco-friendly management of enteroviruses in wastewater |
publisher |
Taylor & Francis Group |
series |
Water Science |
issn |
1110-4929 |
publishDate |
2013-10-01 |
description |
The wastewater treatment facility in El-Manzala (sedimentation basins followed by vegetated cells) was investigated to evaluate its performance as a low cost treatment facility in managing viral pollution. Sedimentation basins are considered to be physical treatment, while vegetated cells are considered as biological treatment. Enteroviruses were detected and determined using two techniques, cell culture as infectivity assay and real time reverse transcriptase-polymerase chain reaction (real time RT-PCR) as a molecular biological technology.
The results revealed that the wastewater treatment facility in El-Manzala showed good performance under Egyptian climatic conditions. The aquatic macrophytes system (biological treatment) showed better performance compared to the sedimentation basins (physical treatment) in removing viral loads. Enteroviruses load at the wetland inlet ranged between 50 and 100% as viral infectivity (VI) by cell culture, and between 4.9 × 104 and 59.5 × 105 gene copies (GC) by real time RT-PCR. The virus load after sedimentation basins ranged between 25 and 50% as VI and between 3.7 × 102 and 4.5 × 104 GC, while after the biological treatment recorded 0% as VI and between 0 and 2 GC.
An empirical model was developed to describe the relationship between the quantity of enteroviruses using molecular biology and infective assay as a predictor variable.
The present study concluded that the wastewater treatment wetland in El-Manzala can be considered as an effective facility in reducing viral contamination of the Bahr El-Baqar drainage water. |
topic |
Constructed wetland Physical treatment Biological treatment Water pollution Enteroviruses PCR |
url |
http://www.sciencedirect.com/science/article/pii/S1110492913000039 |
work_keys_str_mv |
AT abeermhegazy ecofriendlymanagementofenterovirusesinwastewater AT azzahelsalakawy ecofriendlymanagementofenterovirusesinwastewater AT mohamedmshaban ecofriendlymanagementofenterovirusesinwastewater AT mohamedmyehia ecofriendlymanagementofenterovirusesinwastewater AT mohamedsabusalama ecofriendlymanagementofenterovirusesinwastewater |
_version_ |
1724242039595335680 |