Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathway
Neuroprotection is one common strategy to reduce injuries from cerebral ischemia. This study aims to evaluate neuroprotective activity of several representative stilbenes with different substituted groups to preliminarily know about their structure/activity relationship and action mechanisms. Among...
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doaj-cfcccf10510148cfaa09e98e81d518a72021-04-30T07:19:05ZengElsevierJournal of Functional Foods1756-46462020-08-0171104019Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathwayHui Xu0Ruixia Deng1Edmund T.S. Li2Jiangang Shen3Mingfu Wang4School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Hong Kong, ChinaSchool of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Hong Kong, ChinaSchool of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China; Corresponding author.Neuroprotection is one common strategy to reduce injuries from cerebral ischemia. This study aims to evaluate neuroprotective activity of several representative stilbenes with different substituted groups to preliminarily know about their structure/activity relationship and action mechanisms. Among the stilbenes tested, only pinosylvin showed strong neuroprotective effects, evidenced by the decreased cell death in OGD/R-damaged PC12 cells and improved brain function in MCAO/R rats after pinosylvin treatment. Pinosylvin reduced the rate of depolarized cells (low mitochondrial membrane potential) in OGD/R-damaged PC12 cells, implying its role in improving mitochondrial function. Further studies indicated that pinosylvin induced PINK1/Parkin mediated protective mitophagy and activated Nrf2 pathway, suggested by the elevated protein levels of LC3 II, Beclin1, PINK1 and Parkin, and Nrf2 translocation to nucleus. In conclusion, pinosylvin exhibited neuroprotective effects by inducing PINK1/Parkin mediated mitophagy to remove damaged mitochondria and activating Nrf2 pathway to ameliorate oxidative stress-induced mitochondrial dysfunction.http://www.sciencedirect.com/science/article/pii/S1756464620302437Cerebral ischemiaNeuroprotectionPinosylvinMitophagyNrf2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui Xu Ruixia Deng Edmund T.S. Li Jiangang Shen Mingfu Wang |
spellingShingle |
Hui Xu Ruixia Deng Edmund T.S. Li Jiangang Shen Mingfu Wang Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathway Journal of Functional Foods Cerebral ischemia Neuroprotection Pinosylvin Mitophagy Nrf2 |
author_facet |
Hui Xu Ruixia Deng Edmund T.S. Li Jiangang Shen Mingfu Wang |
author_sort |
Hui Xu |
title |
Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathway |
title_short |
Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathway |
title_full |
Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathway |
title_fullStr |
Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathway |
title_full_unstemmed |
Pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing PINK1/Parkin mediated mitophagy and Nrf2 pathway |
title_sort |
pinosylvin provides neuroprotection against cerebral ischemia and reperfusion injury through enhancing pink1/parkin mediated mitophagy and nrf2 pathway |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2020-08-01 |
description |
Neuroprotection is one common strategy to reduce injuries from cerebral ischemia. This study aims to evaluate neuroprotective activity of several representative stilbenes with different substituted groups to preliminarily know about their structure/activity relationship and action mechanisms. Among the stilbenes tested, only pinosylvin showed strong neuroprotective effects, evidenced by the decreased cell death in OGD/R-damaged PC12 cells and improved brain function in MCAO/R rats after pinosylvin treatment. Pinosylvin reduced the rate of depolarized cells (low mitochondrial membrane potential) in OGD/R-damaged PC12 cells, implying its role in improving mitochondrial function. Further studies indicated that pinosylvin induced PINK1/Parkin mediated protective mitophagy and activated Nrf2 pathway, suggested by the elevated protein levels of LC3 II, Beclin1, PINK1 and Parkin, and Nrf2 translocation to nucleus. In conclusion, pinosylvin exhibited neuroprotective effects by inducing PINK1/Parkin mediated mitophagy to remove damaged mitochondria and activating Nrf2 pathway to ameliorate oxidative stress-induced mitochondrial dysfunction. |
topic |
Cerebral ischemia Neuroprotection Pinosylvin Mitophagy Nrf2 |
url |
http://www.sciencedirect.com/science/article/pii/S1756464620302437 |
work_keys_str_mv |
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