iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage
Abstract Background Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a col...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-06-01
|
Series: | BMC Genomics |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12864-020-06797-3 |
id |
doaj-cff5608419d54458beefb2843c8a9a1b |
---|---|
record_format |
Article |
spelling |
doaj-cff5608419d54458beefb2843c8a9a1b2020-11-25T02:51:58ZengBMCBMC Genomics1471-21642020-06-0121111510.1186/s12864-020-06797-3iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storageWen Song0Fengxian Tang1Wenchao Cai2Qin Zhang3Fake Zhou4Ming Ning5Huan Tian6Chunhui Shan7College of Food, Shihezi UniversityCollege of Food, Shihezi UniversityCollege of Food, Shihezi UniversityCollege of Food, Shihezi UniversityCollege of Food, Shihezi UniversityCollege of Food, Shihezi UniversityCollege of Food, Shihezi UniversityCollege of Food, Shihezi UniversityAbstract Background Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results The two kinds of commercial cultivars were exposed to a temperature of 0.5 °C for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.http://link.springer.com/article/10.1186/s12864-020-06797-3CantaloupeCold storageProteomicsiTRAQ |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen Song Fengxian Tang Wenchao Cai Qin Zhang Fake Zhou Ming Ning Huan Tian Chunhui Shan |
spellingShingle |
Wen Song Fengxian Tang Wenchao Cai Qin Zhang Fake Zhou Ming Ning Huan Tian Chunhui Shan iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage BMC Genomics Cantaloupe Cold storage Proteomics iTRAQ |
author_facet |
Wen Song Fengxian Tang Wenchao Cai Qin Zhang Fake Zhou Ming Ning Huan Tian Chunhui Shan |
author_sort |
Wen Song |
title |
iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage |
title_short |
iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage |
title_full |
iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage |
title_fullStr |
iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage |
title_full_unstemmed |
iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage |
title_sort |
itraq-based quantitative proteomics analysis of cantaloupe (cucumis melo var. saccharinus) after cold storage |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2020-06-01 |
description |
Abstract Background Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results The two kinds of commercial cultivars were exposed to a temperature of 0.5 °C for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe. |
topic |
Cantaloupe Cold storage Proteomics iTRAQ |
url |
http://link.springer.com/article/10.1186/s12864-020-06797-3 |
work_keys_str_mv |
AT wensong itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage AT fengxiantang itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage AT wenchaocai itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage AT qinzhang itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage AT fakezhou itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage AT mingning itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage AT huantian itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage AT chunhuishan itraqbasedquantitativeproteomicsanalysisofcantaloupecucumismelovarsaccharinusaftercoldstorage |
_version_ |
1724732138352279552 |