Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers
Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genet...
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Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo
2018-12-01
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doaj-d0163c772e4c4e50b6ecd47ee88417522021-04-30T11:57:12ZengFacultad de Ciencias Agrarias. Universidad Nacional de CuyoRevista de la Facultad de Ciencias Agrarias0370-46611853-86652018-12-01502Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markersEdgardo Carloni0Eliana López Colomba 1Andrea Ribotta2Mariana Quiroga3Exequiel Tommasino4Sabrina Griffa5Karina Grunberg6Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), CIAP-INTA. Av. 11 de Septiembre 4755. C. P. X5020ICA. Córdoba, Argentina.Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), CIAP-INTA. Av. 11 de Septiembre 4755. C. P. X5020ICA. Córdoba, Argentina.Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), CIAP-INTA. Av. 11 de Septiembre 4755. C. P. X5020ICA. Córdoba, Argentina.Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), CIAP-INTA. Av. 11 de Septiembre 4755. C. P. X5020ICA. Córdoba, Argentina.Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), CIAP-INTA. Av. 11 de Septiembre 4755. C. P. X5020ICA. Córdoba, Argentina.Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), CIAP-INTA. Av. 11 de Septiembre 4755. C. P. X5020ICA. Córdoba, Argentina. Consejo Nacional Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, C1425FQB, CABA, Argentina. Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass regenerated plants. Six plants regenerated from in vitro anther culture, via somatic embryogenesis were used, as well as the anther donor genotype (RN 51) as control. Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with a divergence between 5 and 24% from RN 51. Amplification products were observed with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional nucleotides at the 3"² end. The most informative primers were those containing the repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field conditions differed in morphological characteristics among them and with respect to RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro regenerated buffelgrass plants. https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2809Apomixissomatic embryogenesissomaclonal variationanther cultureISSRgenetic diversity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Edgardo Carloni Eliana López Colomba Andrea Ribotta Mariana Quiroga Exequiel Tommasino Sabrina Griffa Karina Grunberg |
spellingShingle |
Edgardo Carloni Eliana López Colomba Andrea Ribotta Mariana Quiroga Exequiel Tommasino Sabrina Griffa Karina Grunberg Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers Revista de la Facultad de Ciencias Agrarias Apomixis somatic embryogenesis somaclonal variation anther culture ISSR genetic diversity |
author_facet |
Edgardo Carloni Eliana López Colomba Andrea Ribotta Mariana Quiroga Exequiel Tommasino Sabrina Griffa Karina Grunberg |
author_sort |
Edgardo Carloni |
title |
Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers |
title_short |
Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers |
title_full |
Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers |
title_fullStr |
Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers |
title_full_unstemmed |
Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers |
title_sort |
analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers |
publisher |
Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo |
series |
Revista de la Facultad de Ciencias Agrarias |
issn |
0370-4661 1853-8665 |
publishDate |
2018-12-01 |
description |
Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass regenerated plants. Six plants regenerated from in vitro anther culture, via somatic embryogenesis were used, as well as the anther donor genotype (RN 51) as control. Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with a divergence between 5 and 24% from RN 51. Amplification products were observed with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional nucleotides at the 3"² end. The most informative primers were those containing the repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field conditions differed in morphological characteristics among them and with respect to RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro regenerated buffelgrass plants.
|
topic |
Apomixis somatic embryogenesis somaclonal variation anther culture ISSR genetic diversity |
url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2809 |
work_keys_str_mv |
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