Applicable Value of AMSS-PCR in Lung Cancer Gene Mutation Detection

• Main Authors: Ke JIN, Xuan XIE, Yuejiang PAN, Kexi WANG, Baishen CHEN, Duoguang WU, Zhuojian SHEN, Minghui WANG, Huizhong ZHANG
• Format: Article
• Language: zho
• Published: Chinese Anti-Cancer Association; Chinese Antituberculosis Association 2018-11-01
• Series: Chinese Journal of Lung Cancer
• Subjects: Sanger sequencing; Amplification Mutation Specific System; Amplification Refractory Mutation System; Quantitative real-time polymerase chain reaction; Lung cancer gene mutation detection
• Online Access: http://dx.doi.org/10.3779/j.issn.1009-3419.2018.11.02
• ISSN: 1009-3419; 1999-6187

Background and objective The detection of driver oncogenes of lung cancer is of great importance. There are various gene detection techniques nowadays which are different from each other. We carried out this study to investigate the specificity and sensitivity of assay panels based on an Amplification Refractory Mutation System-polymerase chain reaction (ARMS-PCR) technique of Amplification Mutation Specific System (AMSS) in detection of lung cancer gene mutation. To estimate the applicable value of assay panels in clinical settings. Methods We collected cancer tissue specimens or fluid specimens from 309 patients. Mutation results were presented for those samples previously detected by ARMS-PCR. In comparison, we carried out AMSS-PCR using (epidermal growth factor receptor, EGFR) assay panel and Six-Alliance assay panel as well as Sanger sequencing. Software SPSS 22.0 (SPSS IBM) was used for statistical analysis. Results The rates of consistency between the results by assay panels and Sanger sequencing or ARMS-PCR were 97.41% and 97.73%, respectively. Besides, EGFR assay panel had higher consistency rates with other detection methods than Six-Alliance assay panel. As for consistency test, the Kappa values of assay panels with Sanger sequencing, assay panels with ARMS-PCR, and ARMS-PCR with Sanger sequencing were 0.946, 0.953, and 0.913, respectively. The receiver operating characteristic curve (ROC) area under curve (AUC) of assay panels was 0.976 referring to Sanger sequencing, and 0.975 as ARMS-PCR was referred to. Conclusion AMSS-PCR can make an optimal cancer gene mutation detection method for clinical settings.