Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
Identification of markers predicting disease outcome is a major clinical issue for non-muscle invasive bladder cancer (NMIBC). The present study aimed to determine the role of the mitochondrial proteins Mitofusin-2 (Mfn2) and caseinolytic protease P (ClpP) in predicting the outcome of NMIBC. The stu...
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doaj-d03620d59c36421b99016014ae7879402021-08-26T13:40:39ZengMDPI AGDiagnostics2075-44182021-08-01111500150010.3390/diagnostics11081500Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder CancerAntonella Cormio0Gian Maria Busetto1Clara Musicco2Francesca Sanguedolce3Beppe Calò4Marco Chirico5Ugo Giovanni Falagario6Giuseppe Carrieri7Claudia Piccoli8Luigi Cormio9Department of Biosciences, Biotechnologies, and Biofarmaceutical, University of Bari, 70126 Bari, ItalyDepartment of Urology and Renal Transplantation, University of Foggia, 71122 Foggia, ItalyCNR Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IBIOM), 70126 Bari, ItalyDepartment of Pathology, University of Foggia, 71122 Foggia, ItalyDepartment of Urology, Bonomo Hospital, 76123 Andria, ItalyDepartment of Urology and Renal Transplantation, University of Foggia, 71122 Foggia, ItalyDepartment of Urology and Renal Transplantation, University of Foggia, 71122 Foggia, ItalyDepartment of Urology and Renal Transplantation, University of Foggia, 71122 Foggia, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Urology and Renal Transplantation, University of Foggia, 71122 Foggia, ItalyIdentification of markers predicting disease outcome is a major clinical issue for non-muscle invasive bladder cancer (NMIBC). The present study aimed to determine the role of the mitochondrial proteins Mitofusin-2 (Mfn2) and caseinolytic protease P (ClpP) in predicting the outcome of NMIBC. The study population consisted of patients scheduled for transurethral resection of bladder tumor upon the clinical diagnosis of bladder cancer (BC). Samples of the main bladder tumor and healthy-looking bladder wall from patients classified as NMIBC were tested for Mfn2 and ClpP. The expression levels of these proteins were correlated to disease recurrence, progression. Mfn2 and ClpP expression levels were significantly higher in lesional than in non-lesional tissue. Low-risk NMIBC had significantly higher Mfn2 expression levels and significantly lower ClpP expression levels than high-risk NMIBC; there were no differences in non-lesional levels of the two proteins. Lesional Mfn2 expression levels were significantly lower in patients who progressed whereas ClpP levels had no impact on any survival outcome. Multivariable analysis adjusting for the EORTC scores showed that Mfn2 downregulation was significantly associated with disease progression. In conclusion, Mfn2 and ClpP proteins were found to be overexpressed in BC as compared to non-lesional bladder tissue and Mfn2 expression predicted disease progression.https://www.mdpi.com/2075-4418/11/8/1500bladder cancerNMIBCMfn2ClpPdisease progressiondisease recurrence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Antonella Cormio Gian Maria Busetto Clara Musicco Francesca Sanguedolce Beppe Calò Marco Chirico Ugo Giovanni Falagario Giuseppe Carrieri Claudia Piccoli Luigi Cormio |
spellingShingle |
Antonella Cormio Gian Maria Busetto Clara Musicco Francesca Sanguedolce Beppe Calò Marco Chirico Ugo Giovanni Falagario Giuseppe Carrieri Claudia Piccoli Luigi Cormio Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer Diagnostics bladder cancer NMIBC Mfn2 ClpP disease progression disease recurrence |
author_facet |
Antonella Cormio Gian Maria Busetto Clara Musicco Francesca Sanguedolce Beppe Calò Marco Chirico Ugo Giovanni Falagario Giuseppe Carrieri Claudia Piccoli Luigi Cormio |
author_sort |
Antonella Cormio |
title |
Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer |
title_short |
Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer |
title_full |
Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer |
title_fullStr |
Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer |
title_full_unstemmed |
Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer |
title_sort |
mitofusin-2 down-regulation predicts progression of non-muscle invasive bladder cancer |
publisher |
MDPI AG |
series |
Diagnostics |
issn |
2075-4418 |
publishDate |
2021-08-01 |
description |
Identification of markers predicting disease outcome is a major clinical issue for non-muscle invasive bladder cancer (NMIBC). The present study aimed to determine the role of the mitochondrial proteins Mitofusin-2 (Mfn2) and caseinolytic protease P (ClpP) in predicting the outcome of NMIBC. The study population consisted of patients scheduled for transurethral resection of bladder tumor upon the clinical diagnosis of bladder cancer (BC). Samples of the main bladder tumor and healthy-looking bladder wall from patients classified as NMIBC were tested for Mfn2 and ClpP. The expression levels of these proteins were correlated to disease recurrence, progression. Mfn2 and ClpP expression levels were significantly higher in lesional than in non-lesional tissue. Low-risk NMIBC had significantly higher Mfn2 expression levels and significantly lower ClpP expression levels than high-risk NMIBC; there were no differences in non-lesional levels of the two proteins. Lesional Mfn2 expression levels were significantly lower in patients who progressed whereas ClpP levels had no impact on any survival outcome. Multivariable analysis adjusting for the EORTC scores showed that Mfn2 downregulation was significantly associated with disease progression. In conclusion, Mfn2 and ClpP proteins were found to be overexpressed in BC as compared to non-lesional bladder tissue and Mfn2 expression predicted disease progression. |
topic |
bladder cancer NMIBC Mfn2 ClpP disease progression disease recurrence |
url |
https://www.mdpi.com/2075-4418/11/8/1500 |
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