Widespread expression of BORIS/CTCFL in normal and cancer cells.
BORIS (CTCFL) is the paralog of CTCF (CCCTC-binding factor; NM_006565), a ubiquitously expressed DNA-binding protein with diverse roles in gene expression and chromatin organisation. BORIS and CTCF have virtually identical zinc finger domains, yet display major differences in their respective C- and...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3139640?pdf=render |
id |
doaj-d036b88b361b4ab183d6f47a2d1f9300 |
---|---|
record_format |
Article |
spelling |
doaj-d036b88b361b4ab183d6f47a2d1f93002020-11-25T01:18:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2239910.1371/journal.pone.0022399Widespread expression of BORIS/CTCFL in normal and cancer cells.Tania A JonesBabatunji W OgunkoladeJaroslaw SzaryJohan AarumMuhammad A MuminShyam PatelChristopher A PieriDenise SheerBORIS (CTCFL) is the paralog of CTCF (CCCTC-binding factor; NM_006565), a ubiquitously expressed DNA-binding protein with diverse roles in gene expression and chromatin organisation. BORIS and CTCF have virtually identical zinc finger domains, yet display major differences in their respective C- and N-terminal regions. Unlike CTCF, BORIS expression has been reported only in the testis and certain malignancies, leading to its classification as a "cancer-testis" antigen. However, the expression pattern of BORIS is both a significant and unresolved question in the field of DNA binding proteins. Here, we identify BORIS in the cytoplasm and nucleus of a wide range of normal and cancer cells. We compare the localization of CTCF and BORIS in the nucleus and demonstrate enrichment of BORIS within the nucleolus, inside the nucleolin core structure and adjacent to fibrillarin in the dense fibrillar component. In contrast, CTCF is not enriched in the nucleolus. Live imaging of cells transiently transfected with GFP tagged BORIS confirmed the nucleolar accumulation of BORIS. While BORIS transcript levels are low compared to CTCF, its protein levels are readily detectable. These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function.http://europepmc.org/articles/PMC3139640?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tania A Jones Babatunji W Ogunkolade Jaroslaw Szary Johan Aarum Muhammad A Mumin Shyam Patel Christopher A Pieri Denise Sheer |
spellingShingle |
Tania A Jones Babatunji W Ogunkolade Jaroslaw Szary Johan Aarum Muhammad A Mumin Shyam Patel Christopher A Pieri Denise Sheer Widespread expression of BORIS/CTCFL in normal and cancer cells. PLoS ONE |
author_facet |
Tania A Jones Babatunji W Ogunkolade Jaroslaw Szary Johan Aarum Muhammad A Mumin Shyam Patel Christopher A Pieri Denise Sheer |
author_sort |
Tania A Jones |
title |
Widespread expression of BORIS/CTCFL in normal and cancer cells. |
title_short |
Widespread expression of BORIS/CTCFL in normal and cancer cells. |
title_full |
Widespread expression of BORIS/CTCFL in normal and cancer cells. |
title_fullStr |
Widespread expression of BORIS/CTCFL in normal and cancer cells. |
title_full_unstemmed |
Widespread expression of BORIS/CTCFL in normal and cancer cells. |
title_sort |
widespread expression of boris/ctcfl in normal and cancer cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
BORIS (CTCFL) is the paralog of CTCF (CCCTC-binding factor; NM_006565), a ubiquitously expressed DNA-binding protein with diverse roles in gene expression and chromatin organisation. BORIS and CTCF have virtually identical zinc finger domains, yet display major differences in their respective C- and N-terminal regions. Unlike CTCF, BORIS expression has been reported only in the testis and certain malignancies, leading to its classification as a "cancer-testis" antigen. However, the expression pattern of BORIS is both a significant and unresolved question in the field of DNA binding proteins. Here, we identify BORIS in the cytoplasm and nucleus of a wide range of normal and cancer cells. We compare the localization of CTCF and BORIS in the nucleus and demonstrate enrichment of BORIS within the nucleolus, inside the nucleolin core structure and adjacent to fibrillarin in the dense fibrillar component. In contrast, CTCF is not enriched in the nucleolus. Live imaging of cells transiently transfected with GFP tagged BORIS confirmed the nucleolar accumulation of BORIS. While BORIS transcript levels are low compared to CTCF, its protein levels are readily detectable. These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function. |
url |
http://europepmc.org/articles/PMC3139640?pdf=render |
work_keys_str_mv |
AT taniaajones widespreadexpressionofborisctcflinnormalandcancercells AT babatunjiwogunkolade widespreadexpressionofborisctcflinnormalandcancercells AT jaroslawszary widespreadexpressionofborisctcflinnormalandcancercells AT johanaarum widespreadexpressionofborisctcflinnormalandcancercells AT muhammadamumin widespreadexpressionofborisctcflinnormalandcancercells AT shyampatel widespreadexpressionofborisctcflinnormalandcancercells AT christopherapieri widespreadexpressionofborisctcflinnormalandcancercells AT denisesheer widespreadexpressionofborisctcflinnormalandcancercells |
_version_ |
1725140497571250176 |