Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field Conditions
The evolution of pathogens in the changing climate raises new challenges for wheat production. Yellow rust is one of the major wheat diseases worldwide, leading to an increased use of fungicides to prevent significant yield losses. The enhancement of the resistance potential of wheat cultivars is a...
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doaj-d03c42206c7d408aaf70ad36908846ef2021-04-02T17:31:56ZengMDPI AGAgronomy2073-43952020-11-01101888188810.3390/agronomy10121888Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field ConditionsSandeep Kumar Kushwaha0Ramesh R. Vetukuri1Firuz Odilbekov2Nidhi Pareek3Tina Henriksson4Aakash Chawade5Department of Plant Breeding, Swedish University of Agricultural Sciences, 230 53 Alnarp, SwedenDepartment of Plant Breeding, Swedish University of Agricultural Sciences, 230 53 Alnarp, SwedenDepartment of Plant Breeding, Swedish University of Agricultural Sciences, 230 53 Alnarp, SwedenDepartment of Microbiology, School of Life Sciences, Central University of Rajasthan, Bandarsindri, Kishangarh, Ajmer, Rajasthan 305801, IndiaLantmännen Lantbruk, 268 31 Svalöv, SwedenDepartment of Plant Breeding, Swedish University of Agricultural Sciences, 230 53 Alnarp, SwedenThe evolution of pathogens in the changing climate raises new challenges for wheat production. Yellow rust is one of the major wheat diseases worldwide, leading to an increased use of fungicides to prevent significant yield losses. The enhancement of the resistance potential of wheat cultivars is a necessary and environmentally friendly solution for sustainable wheat production. In this study, we aimed to identify the differentially expressed genes induced upon yellow rust infection in the field. Reference and de novo based transcriptome analysis was performed among the resistant and susceptible lines of a bi-parental population to study the global transcriptome changes in contrasting wheat genotypes. Based on the analysis, the de novo transcriptome analysis approach was found to be more supportive for field studies. Expression profiles, gene ontology, KEGG pathway analysis and enrichment studies indicated the relation between differentially expressed genes of wheat and yellow rust infection. The h0igh expression of genes related to non-race specific resistance along with pathogen-specific resistance might be a reason for the better resistance ability of a resistant wheat genotype in the field. The targeted metagenomic analysis of wheat samples revealed that <i>Puccinia </i><i>striiformis</i><i> </i><i>tritici</i> was the most dominant pathogen along with other pathogens on the collected leaf material and validating the disease scoring carried out in the field and transcriptomics analyses.https://www.mdpi.com/2073-4395/10/12/1888wheatyellow rustdisease resistancetranscriptomicsdifferential gene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandeep Kumar Kushwaha Ramesh R. Vetukuri Firuz Odilbekov Nidhi Pareek Tina Henriksson Aakash Chawade |
spellingShingle |
Sandeep Kumar Kushwaha Ramesh R. Vetukuri Firuz Odilbekov Nidhi Pareek Tina Henriksson Aakash Chawade Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field Conditions Agronomy wheat yellow rust disease resistance transcriptomics differential gene expression |
author_facet |
Sandeep Kumar Kushwaha Ramesh R. Vetukuri Firuz Odilbekov Nidhi Pareek Tina Henriksson Aakash Chawade |
author_sort |
Sandeep Kumar Kushwaha |
title |
Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field Conditions |
title_short |
Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field Conditions |
title_full |
Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field Conditions |
title_fullStr |
Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field Conditions |
title_full_unstemmed |
Differential Gene Expression Analysis of Wheat Breeding Lines Reveal Molecular Insights in Yellow Rust Resistance under Field Conditions |
title_sort |
differential gene expression analysis of wheat breeding lines reveal molecular insights in yellow rust resistance under field conditions |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2020-11-01 |
description |
The evolution of pathogens in the changing climate raises new challenges for wheat production. Yellow rust is one of the major wheat diseases worldwide, leading to an increased use of fungicides to prevent significant yield losses. The enhancement of the resistance potential of wheat cultivars is a necessary and environmentally friendly solution for sustainable wheat production. In this study, we aimed to identify the differentially expressed genes induced upon yellow rust infection in the field. Reference and de novo based transcriptome analysis was performed among the resistant and susceptible lines of a bi-parental population to study the global transcriptome changes in contrasting wheat genotypes. Based on the analysis, the de novo transcriptome analysis approach was found to be more supportive for field studies. Expression profiles, gene ontology, KEGG pathway analysis and enrichment studies indicated the relation between differentially expressed genes of wheat and yellow rust infection. The h0igh expression of genes related to non-race specific resistance along with pathogen-specific resistance might be a reason for the better resistance ability of a resistant wheat genotype in the field. The targeted metagenomic analysis of wheat samples revealed that <i>Puccinia </i><i>striiformis</i><i> </i><i>tritici</i> was the most dominant pathogen along with other pathogens on the collected leaf material and validating the disease scoring carried out in the field and transcriptomics analyses. |
topic |
wheat yellow rust disease resistance transcriptomics differential gene expression |
url |
https://www.mdpi.com/2073-4395/10/12/1888 |
work_keys_str_mv |
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