Long Non-Coding RNA SNHG14 Regulates SPIN1 Expression to Accelerate Tumor Progression in Non-Small Cell Lung Cancer by Sponging miR-382-5p

• Main Authors: Chen X, Song P, Yao Y, Yang Y
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-09-01
• Series: Cancer Management and Research
• Subjects: lncrna snhg14; mir-382-5p; spin1; tumor progression; nsclc
• Online Access: https://www.dovepress.com/long-non-coding-rna-snhg14-regulates-spin1-expression-to-accelerate-tu-peer-reviewed-article-CMAR

Xiaoliang Chen, Pingan Song, Yuan Yao, Yang Yang Department of Thoracic Surgery, Gansu Gem Flower Hospital, Lanzhou 730060, Gansu, People’s Republic of ChinaCorrespondence: Xiaoliang Chen Department of Thoracic SurgeryGansu Gem Flower Hospital, 733 Welfare East Road, Xigu District, Lanzhou City, Gansu Province 730060, People’s Republic of ChinaTel [phone]Email [e-mail]: Non-small cell lung cancer (NSCLC) is the most common type of lung carcinoma. Long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 (SNHG14) was identified to participate in tumor progression. However, the mechanism and functions of SNHG14 were rarely reported in NSCLC progression.Methods: The relative gene expression was tested by qRT-PCR. Cell viability, apoptosis, migration and invasion were measured by MTT assay, flow cytometry, and transwell migration and invasion assays, respectively. The interactions between miR-382-5p and SNHG14 or SPIN1 were predicted by starBase and confirmed by the dual-luciferase reporter assay and RNA pull-down assay. The protein level of SPIN1 was evaluated by Western blot assay.Results: The levels of SNHG14 and SPIN1 were significantly increased, while the level of miR-382-5p was apparently reduced in NSCLC tissues and cells. SNHG14 was verified to sponge miR-382-5p and SPIN1 was identified as a direct target of miR-382-5p. SNHG14 depletion repressed cell viability, migration and invasion, but induced the apoptotic rate by targeting miR-382-5p. miR-382-5p overexpression blocked cell viability, metastasis and promoted cell apoptosis by regulating SPIN1. SNHG14 silencing down-regulated SPIN1 expression by sponging miR-382-5p.Conclusion: SNHG14 facilitated NSCLC progression by regulating SPIN1 expression via targeting miR-382-5p.Keywords: lncRNA SNHG14, miR-382-5p, SPIN1, tumor progression, NSCLC