Distribution of SCCmec Elements and Presence of Panton-Valentine Leukocidin in Methicillin-Resistant Staphylococcus epidermidis Isolated from Clinical Samples in a University Hospital of Isfahan City, Iran
Introduction: Coagulase Negative Staphylococcus (CoNS) is considered as a major pathogen of nosocomial infections among immunosuppressed patients. Aim: The aim of this study was to identify the types of Staphylococcal Cassette Chromosome mec (SCCmec) and Panton-Valentine Leukocidin (PVL) gene a...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2017-07-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/10258/25518_CE(RA1)_F(SS)_PF1(NE_VT_SS)_PFA(PNE).pdf |
Summary: | Introduction: Coagulase Negative Staphylococcus (CoNS)
is considered as a major pathogen of nosocomial infections
among immunosuppressed patients.
Aim: The aim of this study was to identify the types of
Staphylococcal Cassette Chromosome mec (SCCmec) and
Panton-Valentine Leukocidin (PVL) gene among clinical
Methicillin-Resistant S. epidermidis (MRSE) isolates collected
from Isfahan.
Materials and Methods: This cross-sectional study was
performed from March 2014 to January 2015 at a tertiary care
hospital of Isfahan, Iran. Antimicrobial susceptibility tests of
S. epidermidis isolates were performed by the disc diffusion
method. All the strains were screened for methicillin resistance
based on resistance to cefoxitin (30 μg) disc and presence of
mecA gene. Determination of SCCmec typing and PVL toxin
gene were performed by PCR method. For categorical variables
different groups were compared using the Chi-square test or
Fisher exact test. A p-value of <0.05 was considered significant
for all statistical tests.
Results: The frequency of MRSE was 53.8% according to
the presence of mecA gene. The overall resistance rate was
high with ciprofloxacin (81.4%). PCR analysis showed that
17% (12/70) of MRSE isolate carried the PVL gene and 43%
(30/70) were SCCmec type I; 11.4% (8/70) were type II; and
34.2% (24/70) were type IV, whereas, 11.4% (8/70) of the MRSE
isolates could not be typed.
Conclusion: SCCmec type I was the major type of SCCmec,
which indicates an emergence of this SCCmec type in the
studied medical centers. Increased prevalence of SCCmec types
in community is cause of an increase in antibiotic resistance
among microorganisms. |
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ISSN: | 2249-782X 0973-709X |