Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication
The complete and accurate replication of the genome is a crucial aspect of cell proliferation that is often perturbed during oncogenesis. Replication stress arising from a variety of obstacles to replication fork progression and processivity is an important contributor to genome destabilization. Acc...
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doaj-d05a7fd9f0d34858bb4f49521824e5852020-11-25T00:42:14ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-09-011910290910.3390/ijms19102909ijms19102909Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA ReplicationMaïlyn Yates0Alexandre Maréchal1Department of Biology, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, CanadaDepartment of Biology, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, CanadaThe complete and accurate replication of the genome is a crucial aspect of cell proliferation that is often perturbed during oncogenesis. Replication stress arising from a variety of obstacles to replication fork progression and processivity is an important contributor to genome destabilization. Accordingly, cells mount a complex response to this stress that allows the stabilization and restart of stalled replication forks and enables the full duplication of the genetic material. This response articulates itself on three important platforms, Replication Protein A/RPA-coated single-stranded DNA, the DNA polymerase processivity clamp PCNA and the FANCD2/I Fanconi Anemia complex. On these platforms, the recruitment, activation and release of a variety of genome maintenance factors is regulated by post-translational modifications including mono- and poly-ubiquitylation. Here, we review recent insights into the control of replication fork stability and restart by the ubiquitin system during replication stress with a particular focus on human cells. We highlight the roles of E3 ubiquitin ligases, ubiquitin readers and deubiquitylases that provide the required flexibility at stalled forks to select the optimal restart pathways and rescue genome stability during stressful conditions.http://www.mdpi.com/1422-0067/19/10/2909DNA replication stressgenome stabilityubiquitinreplication fork restarttranslesion synthesistemplate-switchinghomologous recombinationFanconi Anemia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maïlyn Yates Alexandre Maréchal |
spellingShingle |
Maïlyn Yates Alexandre Maréchal Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication International Journal of Molecular Sciences DNA replication stress genome stability ubiquitin replication fork restart translesion synthesis template-switching homologous recombination Fanconi Anemia |
author_facet |
Maïlyn Yates Alexandre Maréchal |
author_sort |
Maïlyn Yates |
title |
Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication |
title_short |
Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication |
title_full |
Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication |
title_fullStr |
Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication |
title_full_unstemmed |
Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication |
title_sort |
ubiquitylation at the fork: making and breaking chains to complete dna replication |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-09-01 |
description |
The complete and accurate replication of the genome is a crucial aspect of cell proliferation that is often perturbed during oncogenesis. Replication stress arising from a variety of obstacles to replication fork progression and processivity is an important contributor to genome destabilization. Accordingly, cells mount a complex response to this stress that allows the stabilization and restart of stalled replication forks and enables the full duplication of the genetic material. This response articulates itself on three important platforms, Replication Protein A/RPA-coated single-stranded DNA, the DNA polymerase processivity clamp PCNA and the FANCD2/I Fanconi Anemia complex. On these platforms, the recruitment, activation and release of a variety of genome maintenance factors is regulated by post-translational modifications including mono- and poly-ubiquitylation. Here, we review recent insights into the control of replication fork stability and restart by the ubiquitin system during replication stress with a particular focus on human cells. We highlight the roles of E3 ubiquitin ligases, ubiquitin readers and deubiquitylases that provide the required flexibility at stalled forks to select the optimal restart pathways and rescue genome stability during stressful conditions. |
topic |
DNA replication stress genome stability ubiquitin replication fork restart translesion synthesis template-switching homologous recombination Fanconi Anemia |
url |
http://www.mdpi.com/1422-0067/19/10/2909 |
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