Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
Background: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression...
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doaj-d065a3a5f2b84279a1895ff36cf892322020-11-25T01:28:23ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-03-01216223310.3390/ijms21062233ijms21062233Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep ApneaDavid Sanz-Rubio0Arianne Sanz1Luis Varona2Rosa Bolea3Marta Forner4Ana V. Gil5Pablo Cubero6Marta Marin-Oto7Inmaculada Martin-Burriel8Jose M. Marin9Translational Research Unit, Hospital Universitario Miguel Servet, Instituto de Investigación Sanitaria de Aragón (IISAragón), 50009 Zaragoza, SpainBiochemical Genetics Laboratory (LAGENBIO), Instituto Agroalimentario de Aragón (IA2), Instituto de Investigación Sanitaria 0de Aragón (IISAragón), University of Zaragoza, 50013 Zaragoza, SpainDepartamento de Anatomía Embriología y Genética Animal, Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza, 50013 Zaragoza, SpainDepartamento de Patología Animal, Facultad de Veterinaria, Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza, 50013 Zaragoza, SpainTranslational Research Unit, Hospital Universitario Miguel Servet, Instituto de Investigación Sanitaria de Aragón (IISAragón), 50009 Zaragoza, SpainTranslational Research Unit, Hospital Universitario Miguel Servet, Instituto de Investigación Sanitaria de Aragón (IISAragón), 50009 Zaragoza, SpainTranslational Research Unit, Hospital Universitario Miguel Servet, Instituto de Investigación Sanitaria de Aragón (IISAragón), 50009 Zaragoza, SpainTranslational Research Unit, Hospital Universitario Miguel Servet, Instituto de Investigación Sanitaria de Aragón (IISAragón), 50009 Zaragoza, SpainBiochemical Genetics Laboratory (LAGENBIO), Instituto Agroalimentario de Aragón (IA2), Instituto de Investigación Sanitaria 0de Aragón (IISAragón), University of Zaragoza, 50013 Zaragoza, SpainTranslational Research Unit, Hospital Universitario Miguel Servet, Instituto de Investigación Sanitaria de Aragón (IISAragón), 50009 Zaragoza, SpainBackground: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression of this gene have not been tested in adults with OSA. Methods: Plasma samples from subjects without comorbid conditions other than OSA were analyzed (the Epigenetics Status and Subclinical Atherosclerosis in Obstructive Sleep Apnea (EPIOSA) Study: NCT02131610). In 16 patients with severe OSA (Apnea-Hypopnea Index—AHI- > 30 events/h) and seven matched controls (AHI < 5), methylation of FOXP3 gen was evaluated by PCR of the promoter and by pyrosequencing of the intron 1 Treg-specific demethylated region (TSDR). In another 74 patients with OSA (AHI > 10) and 31 controls, we quantified FOXP3 protein expression by ELISA and gene expression by quantitative real-time PCR. C-reactive protein (CRP) and plasma Treg cells were also evaluated. Results: Neither the levels of the promoter nor the TSDR demethylated region were different between controls and patients with OSA, whether they were grouped by normal or high CRP. FOXP3 protein and mRNA expression did not differ between groups. Conclusions: FOXP3 methylation or its expression is not altered in adults with OSA, whatever their inflammatory status.https://www.mdpi.com/1422-0067/21/6/2233obstructive sleep apneafoxp3 methylationfoxp3 expressionepigenetics. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David Sanz-Rubio Arianne Sanz Luis Varona Rosa Bolea Marta Forner Ana V. Gil Pablo Cubero Marta Marin-Oto Inmaculada Martin-Burriel Jose M. Marin |
spellingShingle |
David Sanz-Rubio Arianne Sanz Luis Varona Rosa Bolea Marta Forner Ana V. Gil Pablo Cubero Marta Marin-Oto Inmaculada Martin-Burriel Jose M. Marin Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea International Journal of Molecular Sciences obstructive sleep apnea foxp3 methylation foxp3 expression epigenetics. |
author_facet |
David Sanz-Rubio Arianne Sanz Luis Varona Rosa Bolea Marta Forner Ana V. Gil Pablo Cubero Marta Marin-Oto Inmaculada Martin-Burriel Jose M. Marin |
author_sort |
David Sanz-Rubio |
title |
Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea |
title_short |
Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea |
title_full |
Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea |
title_fullStr |
Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea |
title_full_unstemmed |
Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea |
title_sort |
forkhead box p3 methylation and expression in men with obstructive sleep apnea |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-03-01 |
description |
Background: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression of this gene have not been tested in adults with OSA. Methods: Plasma samples from subjects without comorbid conditions other than OSA were analyzed (the Epigenetics Status and Subclinical Atherosclerosis in Obstructive Sleep Apnea (EPIOSA) Study: NCT02131610). In 16 patients with severe OSA (Apnea-Hypopnea Index—AHI- > 30 events/h) and seven matched controls (AHI < 5), methylation of FOXP3 gen was evaluated by PCR of the promoter and by pyrosequencing of the intron 1 Treg-specific demethylated region (TSDR). In another 74 patients with OSA (AHI > 10) and 31 controls, we quantified FOXP3 protein expression by ELISA and gene expression by quantitative real-time PCR. C-reactive protein (CRP) and plasma Treg cells were also evaluated. Results: Neither the levels of the promoter nor the TSDR demethylated region were different between controls and patients with OSA, whether they were grouped by normal or high CRP. FOXP3 protein and mRNA expression did not differ between groups. Conclusions: FOXP3 methylation or its expression is not altered in adults with OSA, whatever their inflammatory status. |
topic |
obstructive sleep apnea foxp3 methylation foxp3 expression epigenetics. |
url |
https://www.mdpi.com/1422-0067/21/6/2233 |
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