Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland

• Main Authors: Angelika Ziegler, Magdalena Kawka, Marcin Przybys, Teresa Doroszewska, Urszula Skomra, Ute Kastirr, Jaroslav Matoušek, Jörg Schubert
• Format: Article
• Language: deu
• Published: Eugen Ulmer KG 2014-07-01
• Series: Journal für Kulturpflanzen
• Subjects: Hop latent virus; hop latent viroid; hop stunt viroid; RT-PCR; real time RT-PCR; ELISA
• Online Access: https://ojs.openagrar.de/index.php/Kulturpflanzenjournal/article/view/12530
• ISSN: 1867-0911; 1867-0938

Monitoring the occurrence of virus diseases in plants is important for the implementation of early control measures and prevention of further disease spread. In Poland, in 2004 a health programme for hop was started to eliminate viruses and viroids. In 2012/13, in vitro plants, samples from the IUNG-PIB experimental station and commercial hop gardens in Poland were tested for Hop latent virus (HpLV), and Hop latent and Hop stunt viroids (HpLVd and HpSVd). For virus testing, RT-PCR and ELISA methods were used. In order to detect hop viroids, RT-PCR was employed. The overall incidence of HpLV and hop viroids was lower than reported before the start of the programme. Cloning and sequencing revealed that the HpLV and the HpLVd from Polish sources are very similar to the type sequences and the Czech sources. DOI: 10.5073/JfK.2014.07.04, https://doi.org/10.5073/JfK.2014.07.04