N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria

Cyanobactins are a recently recognized group of ribosomal cyclic peptides produced by cyanobacteria, which have been studied because of their interesting biological activities. Here, we have used a PCR-based approach to detect the N-terminal protease (A) gene from cyanobactin synthetase gene cluster...

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Main Authors:	Joana Martins, Pedro N. Leão, Vitor Ramos, Vitor Vasconcelos
Format:	Article
Language:	English
Published:	MDPI AG 2013-12-01
Series:	Marine Drugs
Subjects:	cyanobacteria cyanobactins bioactive compounds phylogeny
Online Access:	http://www.mdpi.com/1660-3397/11/12/4902

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record_format	Article
spelling	doaj-d07dd282047649eda607c24b99e848c32020-11-24T22:35:02ZengMDPI AGMarine Drugs1660-33972013-12-0111124902491610.3390/md11124902md11124902N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in CyanobacteriaJoana Martins0Pedro N. Leão1Vitor Ramos2Vitor Vasconcelos3Faculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4169-007, PortugalCIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, Porto 4050-123, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4169-007, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4169-007, PortugalCyanobactins are a recently recognized group of ribosomal cyclic peptides produced by cyanobacteria, which have been studied because of their interesting biological activities. Here, we have used a PCR-based approach to detect the N-terminal protease (A) gene from cyanobactin synthetase gene clusters, in a set of diverse cyanobacteria from our culture collection (Laboratory of Ecotoxicology, Genomics and Evolution (LEGE) CC). Homologues of this gene were found in Microcystis and Rivularia strains, and for the first time in Cuspidothrix, Phormidium and Sphaerospermopsis strains. Phylogenetic relationships inferred from available A-gene sequences, including those obtained in this work, revealed two new groups of phylotypes, harboring Phormidium, Sphaerospermopsis and Rivularia LEGE isolates. Thus, this study shows that, using underexplored cyanobacterial strains, it is still possible to expand the known genetic diversity of genes involved in cyanobactin biosynthesis.http://www.mdpi.com/1660-3397/11/12/4902cyanobacteriacyanobactinsbioactive compoundsphylogeny
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Joana Martins Pedro N. Leão Vitor Ramos Vitor Vasconcelos
spellingShingle	Joana Martins Pedro N. Leão Vitor Ramos Vitor Vasconcelos N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria Marine Drugs cyanobacteria cyanobactins bioactive compounds phylogeny
author_facet	Joana Martins Pedro N. Leão Vitor Ramos Vitor Vasconcelos
author_sort	Joana Martins
title	N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria
title_short	N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria
title_full	N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria
title_fullStr	N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria
title_full_unstemmed	N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria
title_sort	n-terminal protease gene phylogeny reveals the potential for novel cyanobactin diversity in cyanobacteria
publisher	MDPI AG
series	Marine Drugs
issn	1660-3397
publishDate	2013-12-01
description	Cyanobactins are a recently recognized group of ribosomal cyclic peptides produced by cyanobacteria, which have been studied because of their interesting biological activities. Here, we have used a PCR-based approach to detect the N-terminal protease (A) gene from cyanobactin synthetase gene clusters, in a set of diverse cyanobacteria from our culture collection (Laboratory of Ecotoxicology, Genomics and Evolution (LEGE) CC). Homologues of this gene were found in Microcystis and Rivularia strains, and for the first time in Cuspidothrix, Phormidium and Sphaerospermopsis strains. Phylogenetic relationships inferred from available A-gene sequences, including those obtained in this work, revealed two new groups of phylotypes, harboring Phormidium, Sphaerospermopsis and Rivularia LEGE isolates. Thus, this study shows that, using underexplored cyanobacterial strains, it is still possible to expand the known genetic diversity of genes involved in cyanobactin biosynthesis.
topic	cyanobacteria cyanobactins bioactive compounds phylogeny
url	http://www.mdpi.com/1660-3397/11/12/4902
work_keys_str_mv	AT joanamartins nterminalproteasegenephylogenyrevealsthepotentialfornovelcyanobactindiversityincyanobacteria AT pedronleao nterminalproteasegenephylogenyrevealsthepotentialfornovelcyanobactindiversityincyanobacteria AT vitorramos nterminalproteasegenephylogenyrevealsthepotentialfornovelcyanobactindiversityincyanobacteria AT vitorvasconcelos nterminalproteasegenephylogenyrevealsthepotentialfornovelcyanobactindiversityincyanobacteria
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N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria

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