Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth disease

<p>Abstract</p> <p>Background</p> <p>Hand-foot-and-mouth disease (HFMD) is caused mainly by the human enterovirus type 71 (HEV71) and the Coxsackievirus A group type 16 (CVA16). Large outbreaks of disease have occurred frequently in the Asia-Pacific region. Reliable met...

Full description

Bibliographic Details
Main Authors: Yu Nan, Guo Min, He Si-Jie, Pan Yu-Xian, Chen Xin-Xin, Ding Xi-Xia, Hao Wei, Wang Ya-Di, Ge Sheng-Xiang, Xia Ning-Shao, Che Xiao-Yan
Format: Article
Language:English
Published: BMC 2012-01-01
Series:Virology Journal
Subjects:
Online Access:http://www.virologyj.com/content/9/1/12
id doaj-d098e1566fc84726a11b15e84eeb3adf
record_format Article
spelling doaj-d098e1566fc84726a11b15e84eeb3adf2020-11-24T21:33:41ZengBMCVirology Journal1743-422X2012-01-01911210.1186/1743-422X-9-12Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth diseaseYu NanGuo MinHe Si-JiePan Yu-XianChen Xin-XinDing Xi-XiaHao WeiWang Ya-DiGe Sheng-XiangXia Ning-ShaoChe Xiao-Yan<p>Abstract</p> <p>Background</p> <p>Hand-foot-and-mouth disease (HFMD) is caused mainly by the human enterovirus type 71 (HEV71) and the Coxsackievirus A group type 16 (CVA16). Large outbreaks of disease have occurred frequently in the Asia-Pacific region. Reliable methods are needed for diagnosis of HFMD in childen. IgM-capture ELISA, with its notable advantages of convenience and low cost, provides a potentially frontline assay. We aimed to evaluate the newly developed IgM-capture ELISAs for HEV71 and CVA16 in the diagnosis of HFMD, and to measure the kinetics of IgM over the course of HEV71 or CVA16 infections.</p> <p>Results</p> <p>We mapped, for the first time, the kinetics of IgM in HEV71 and CVA16 infection. HEV71- and CVA16-IgM were both detectable in some patients on day 1 of illness, and in 100% of patients by day 5 (HEV71) and day 8 (CVA16) respectively; both IgMs persisted for several weeks. The IgM detection rates were 90.2% (138 of 153 sera) and 68.0% (66 of 97 sera) for HEV71 and CVA16 infections, respectively, during the first 7 days of diseases. During the first 90 days after onset these values were 93.6% (233 of 249 sera) and 72.8% (91 of 125 sera) for HEV71 and CVA16 infections, respectively. Some cross-reactivity was observed between HEV71- and CVA16-IgM ELISAs. HEV71-IgM was positive in 38 of 122 (31.1%) CVA16 infections, 14 of 49 (28.6%) other enteroviral infections and 2 of 105 (1.9%) for other respiratory virus infected sera. Similarly, CVA16-IgM was apparently positive in 58 of 211 (27.5%) HEV71 infections, 16 of 48 (33.3%) other enterovirus infections and 3 of 105 (2.9%) other respiratory virus infected sera. Nevertheless, the ELISA yielded the higher OD<sub>450 </sub>value of main antibody than that of cross-reaction antibody, successfully identifying the enteroviral infection in 96.6% (HEV71) and 91.7% (CVA16) cases. When blood and rectal swabs were collected on the same day, the data showed that the agreement between IgM-capture ELISA and real-time RT-PCR in HEV71 was high (Kappa value = 0.729) while CVA16 somewhat lower (Kappa value = 0.300).</p> <p>Conclusions</p> <p>HEV71- and CVA16-IgM ELISAs can be deployed successfully as a convenient and cost-effective diagnostic tool for HFMD in clinical laboratories.</p> http://www.virologyj.com/content/9/1/12EnterovirusHEV71CVA16Hand-Foot-and-Mouth DiseaseIgM-capture ELISACross-reactivity
collection DOAJ
language English
format Article
sources DOAJ
author Yu Nan
Guo Min
He Si-Jie
Pan Yu-Xian
Chen Xin-Xin
Ding Xi-Xia
Hao Wei
Wang Ya-Di
Ge Sheng-Xiang
Xia Ning-Shao
Che Xiao-Yan
spellingShingle Yu Nan
Guo Min
He Si-Jie
Pan Yu-Xian
Chen Xin-Xin
Ding Xi-Xia
Hao Wei
Wang Ya-Di
Ge Sheng-Xiang
Xia Ning-Shao
Che Xiao-Yan
Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth disease
Virology Journal
Enterovirus
HEV71
CVA16
Hand-Foot-and-Mouth Disease
IgM-capture ELISA
Cross-reactivity
author_facet Yu Nan
Guo Min
He Si-Jie
Pan Yu-Xian
Chen Xin-Xin
Ding Xi-Xia
Hao Wei
Wang Ya-Di
Ge Sheng-Xiang
Xia Ning-Shao
Che Xiao-Yan
author_sort Yu Nan
title Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth disease
title_short Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth disease
title_full Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth disease
title_fullStr Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth disease
title_full_unstemmed Evaluation of human enterovirus 71 and coxsackievirus A16 specific immunoglobulin M antibodies for diagnosis of hand-foot-and-mouth disease
title_sort evaluation of human enterovirus 71 and coxsackievirus a16 specific immunoglobulin m antibodies for diagnosis of hand-foot-and-mouth disease
publisher BMC
series Virology Journal
issn 1743-422X
publishDate 2012-01-01
description <p>Abstract</p> <p>Background</p> <p>Hand-foot-and-mouth disease (HFMD) is caused mainly by the human enterovirus type 71 (HEV71) and the Coxsackievirus A group type 16 (CVA16). Large outbreaks of disease have occurred frequently in the Asia-Pacific region. Reliable methods are needed for diagnosis of HFMD in childen. IgM-capture ELISA, with its notable advantages of convenience and low cost, provides a potentially frontline assay. We aimed to evaluate the newly developed IgM-capture ELISAs for HEV71 and CVA16 in the diagnosis of HFMD, and to measure the kinetics of IgM over the course of HEV71 or CVA16 infections.</p> <p>Results</p> <p>We mapped, for the first time, the kinetics of IgM in HEV71 and CVA16 infection. HEV71- and CVA16-IgM were both detectable in some patients on day 1 of illness, and in 100% of patients by day 5 (HEV71) and day 8 (CVA16) respectively; both IgMs persisted for several weeks. The IgM detection rates were 90.2% (138 of 153 sera) and 68.0% (66 of 97 sera) for HEV71 and CVA16 infections, respectively, during the first 7 days of diseases. During the first 90 days after onset these values were 93.6% (233 of 249 sera) and 72.8% (91 of 125 sera) for HEV71 and CVA16 infections, respectively. Some cross-reactivity was observed between HEV71- and CVA16-IgM ELISAs. HEV71-IgM was positive in 38 of 122 (31.1%) CVA16 infections, 14 of 49 (28.6%) other enteroviral infections and 2 of 105 (1.9%) for other respiratory virus infected sera. Similarly, CVA16-IgM was apparently positive in 58 of 211 (27.5%) HEV71 infections, 16 of 48 (33.3%) other enterovirus infections and 3 of 105 (2.9%) other respiratory virus infected sera. Nevertheless, the ELISA yielded the higher OD<sub>450 </sub>value of main antibody than that of cross-reaction antibody, successfully identifying the enteroviral infection in 96.6% (HEV71) and 91.7% (CVA16) cases. When blood and rectal swabs were collected on the same day, the data showed that the agreement between IgM-capture ELISA and real-time RT-PCR in HEV71 was high (Kappa value = 0.729) while CVA16 somewhat lower (Kappa value = 0.300).</p> <p>Conclusions</p> <p>HEV71- and CVA16-IgM ELISAs can be deployed successfully as a convenient and cost-effective diagnostic tool for HFMD in clinical laboratories.</p>
topic Enterovirus
HEV71
CVA16
Hand-Foot-and-Mouth Disease
IgM-capture ELISA
Cross-reactivity
url http://www.virologyj.com/content/9/1/12
work_keys_str_mv AT yunan evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT guomin evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT hesijie evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT panyuxian evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT chenxinxin evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT dingxixia evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT haowei evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT wangyadi evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT geshengxiang evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT xianingshao evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
AT chexiaoyan evaluationofhumanenterovirus71andcoxsackievirusa16specificimmunoglobulinmantibodiesfordiagnosisofhandfootandmouthdisease
_version_ 1725952476999319552