On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling
Mammalian tears are produced by lacrimal glands to protect eyes and may function in chemical communication and immunity. Recent studies on the house mouse chemical signalling revealed that major urinary proteins (MUPs) are not individually unique in Mus musculus musculus. This fact stimulated us to...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2017-07-01
|
Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/3541.pdf |
id |
doaj-d09b9f4e82d4414a96fef78a71c38d3e |
---|---|
record_format |
Article |
spelling |
doaj-d09b9f4e82d4414a96fef78a71c38d3e2020-11-24T21:29:10ZengPeerJ Inc.PeerJ2167-83592017-07-015e354110.7717/peerj.3541On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signallingRomana Stopkova0Petr Klempt1Barbora Kuntova2Pavel Stopka3BIOCEV group, Department of Zoology, Faculty of Science, Charles University, Prague, Czech RepublicBIOCEV group, Department of Zoology, Faculty of Science, Charles University, Prague, Czech RepublicBIOCEV group, Department of Zoology, Faculty of Science, Charles University, Prague, Czech RepublicBIOCEV group, Department of Zoology, Faculty of Science, Charles University, Prague, Czech RepublicMammalian tears are produced by lacrimal glands to protect eyes and may function in chemical communication and immunity. Recent studies on the house mouse chemical signalling revealed that major urinary proteins (MUPs) are not individually unique in Mus musculus musculus. This fact stimulated us to look for other sexually dimorphic proteins that may—in combination with MUPs—contribute to a pool of chemical signals in tears. MUPs and other lipocalins including odorant binding proteins (OBPs) have the capacity to selectively transport volatile organic compounds (VOCs) in their eight-stranded beta barrel, thus we have generated the tear proteome of the house mouse to detect a wider pool of proteins that may be involved in chemical signalling. We have detected significant male-biased (7.8%) and female-biased (7%) proteins in tears. Those proteins that showed the most elevated sexual dimorphisms were highly expressed and belong to MUP, OBP, ESP (i.e., exocrine gland-secreted peptides), and SCGB/ABP (i.e., secretoglobin) families. Thus, tears may have the potential to elicit sex-specific signals in combination by different proteins. Some tear lipocalins are not sexually dimorphic—with MUP20/darcin and OBP6 being good examples—and because all proteins may flow with tears through nasolacrimal ducts to nasal and oral cavities we suggest that their roles are wider than originally thought. Also, we have also detected several sexually dimorphic bactericidal proteins, thus further supporting an idea that males and females may have adopted alternative strategies in controlling microbiota thus yielding different VOC profiles.https://peerj.com/articles/3541.pdfPheromoneLipocalinsToxic waste hypothesisTearsSex dimorphismSecretoglobins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Romana Stopkova Petr Klempt Barbora Kuntova Pavel Stopka |
spellingShingle |
Romana Stopkova Petr Klempt Barbora Kuntova Pavel Stopka On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling PeerJ Pheromone Lipocalins Toxic waste hypothesis Tears Sex dimorphism Secretoglobins |
author_facet |
Romana Stopkova Petr Klempt Barbora Kuntova Pavel Stopka |
author_sort |
Romana Stopkova |
title |
On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling |
title_short |
On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling |
title_full |
On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling |
title_fullStr |
On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling |
title_full_unstemmed |
On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling |
title_sort |
on the tear proteome of the house mouse (mus musculus musculus) in relation to chemical signalling |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2017-07-01 |
description |
Mammalian tears are produced by lacrimal glands to protect eyes and may function in chemical communication and immunity. Recent studies on the house mouse chemical signalling revealed that major urinary proteins (MUPs) are not individually unique in Mus musculus musculus. This fact stimulated us to look for other sexually dimorphic proteins that may—in combination with MUPs—contribute to a pool of chemical signals in tears. MUPs and other lipocalins including odorant binding proteins (OBPs) have the capacity to selectively transport volatile organic compounds (VOCs) in their eight-stranded beta barrel, thus we have generated the tear proteome of the house mouse to detect a wider pool of proteins that may be involved in chemical signalling. We have detected significant male-biased (7.8%) and female-biased (7%) proteins in tears. Those proteins that showed the most elevated sexual dimorphisms were highly expressed and belong to MUP, OBP, ESP (i.e., exocrine gland-secreted peptides), and SCGB/ABP (i.e., secretoglobin) families. Thus, tears may have the potential to elicit sex-specific signals in combination by different proteins. Some tear lipocalins are not sexually dimorphic—with MUP20/darcin and OBP6 being good examples—and because all proteins may flow with tears through nasolacrimal ducts to nasal and oral cavities we suggest that their roles are wider than originally thought. Also, we have also detected several sexually dimorphic bactericidal proteins, thus further supporting an idea that males and females may have adopted alternative strategies in controlling microbiota thus yielding different VOC profiles. |
topic |
Pheromone Lipocalins Toxic waste hypothesis Tears Sex dimorphism Secretoglobins |
url |
https://peerj.com/articles/3541.pdf |
work_keys_str_mv |
AT romanastopkova onthetearproteomeofthehousemousemusmusculusmusculusinrelationtochemicalsignalling AT petrklempt onthetearproteomeofthehousemousemusmusculusmusculusinrelationtochemicalsignalling AT barborakuntova onthetearproteomeofthehousemousemusmusculusmusculusinrelationtochemicalsignalling AT pavelstopka onthetearproteomeofthehousemousemusmusculusmusculusinrelationtochemicalsignalling |
_version_ |
1725967002034503680 |