Clavulanic acid improves ethanol withdrawal symptoms in rats
<em><strong>Objective(s):</strong></em> Ethanol withdrawal following chronic use, is an important challenge clinically. In this study, the effect of clavulanic acid was evaluated on the symptoms of ethanol withdrawal in rats.<br /> <em><strong>Materials and...
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doaj-d0a788df9a394612b61cb6f5d71a382a2020-11-25T03:06:13ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742020-06-0123673073610.22038/ijbms.2020.39129.928715478Clavulanic acid improves ethanol withdrawal symptoms in ratsEhsan Mohebi0Mehdi Molavi1Mohammad Mohammadzadeh2Hossein Hosseinzadeh3Bahareh Amin4Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar, IranDepartment of Internal Medicine, Sabzevar University of Medical Sciences, Mashhad, I.R. IranCellular and Molecular Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran|Department of physiology and pharmacology, Mashhad University of Medical Sciences, Mashhad, I.R. IranDepartment of Pharmacodynamics and Toxicology, Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I.R. IranCellular and Molecular Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran<em><strong>Objective(s):</strong></em> Ethanol withdrawal following chronic use, is an important challenge clinically. In this study, the effect of clavulanic acid was evaluated on the symptoms of ethanol withdrawal in rats.<br /> <em><strong>Materials and Methods:</strong></em> Alcohol dependence was induced by the gavage of ethanol (10% v/v, 2 g/kg), twice daily for 10 days. Clavulanic acid (10, 20, 40, and 80 mg/kg) was administered concurrently with ethanol (sub-acute study), or a single dose after ethanol withdrawal (acute study). Six hours after the last dose of ethanol, anxiety was assessed by the elevated plus-maze (EPM). Seizure-like behavior was evaluated by a sub-convulsive dose of pentylenetetrazol (PTZ, 25 mg/kg/IP). Locomotor activity and motor coordination were measured by the open field and rotarod tests, respectively. Lipid peroxidation marker and antioxidant content were assessed through measuring malondialdehyde (MDA) and glutathione (GSH), respectively.<br /><em><strong>Results:</strong></em> The number of entries and time spent on the open arms of EPM decreased during the withdrawal state. Motor coordination and locomotor activity were significantly decreased. In the sub-acute study, clavulanic acid 80 mg/kg increased time spent and the number of entries to the open arms of EPM, in withdrawn animals. Both motor incoordination and locomotor activity reduction were normalized by clavulanic acid (10, 20, 40 and 80 mg/kg). Withdrawal-induced PTZ kindling seizure was also suppressed by all of the doses. MDA increased, while GSH decreased after withdrawal. Clavulanic acid attenuated such changes.<br /><em><strong>Conclusion:</strong></em> Clavulanic acid could prevent the development of alcohol withdrawal-induced anxiety and seizure. Alcohol withdrawal causes oxidative stress which can be prevented by clavulanic acid.http://ijbms.mums.ac.ir/article_15478_662424c864ee8d42c41ca83ad818c32c.pdfalcohol withdrawalclavulanic acidelevated plus mazeoxidative stresspentylenetetrazolrat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ehsan Mohebi Mehdi Molavi Mohammad Mohammadzadeh Hossein Hosseinzadeh Bahareh Amin |
spellingShingle |
Ehsan Mohebi Mehdi Molavi Mohammad Mohammadzadeh Hossein Hosseinzadeh Bahareh Amin Clavulanic acid improves ethanol withdrawal symptoms in rats Iranian Journal of Basic Medical Sciences alcohol withdrawal clavulanic acid elevated plus maze oxidative stress pentylenetetrazol rat |
author_facet |
Ehsan Mohebi Mehdi Molavi Mohammad Mohammadzadeh Hossein Hosseinzadeh Bahareh Amin |
author_sort |
Ehsan Mohebi |
title |
Clavulanic acid improves ethanol withdrawal symptoms in rats |
title_short |
Clavulanic acid improves ethanol withdrawal symptoms in rats |
title_full |
Clavulanic acid improves ethanol withdrawal symptoms in rats |
title_fullStr |
Clavulanic acid improves ethanol withdrawal symptoms in rats |
title_full_unstemmed |
Clavulanic acid improves ethanol withdrawal symptoms in rats |
title_sort |
clavulanic acid improves ethanol withdrawal symptoms in rats |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2020-06-01 |
description |
<em><strong>Objective(s):</strong></em> Ethanol withdrawal following chronic use, is an important challenge clinically. In this study, the effect of clavulanic acid was evaluated on the symptoms of ethanol withdrawal in rats.<br /> <em><strong>Materials and Methods:</strong></em> Alcohol dependence was induced by the gavage of ethanol (10% v/v, 2 g/kg), twice daily for 10 days. Clavulanic acid (10, 20, 40, and 80 mg/kg) was administered concurrently with ethanol (sub-acute study), or a single dose after ethanol withdrawal (acute study). Six hours after the last dose of ethanol, anxiety was assessed by the elevated plus-maze (EPM). Seizure-like behavior was evaluated by a sub-convulsive dose of pentylenetetrazol (PTZ, 25 mg/kg/IP). Locomotor activity and motor coordination were measured by the open field and rotarod tests, respectively. Lipid peroxidation marker and antioxidant content were assessed through measuring malondialdehyde (MDA) and glutathione (GSH), respectively.<br /><em><strong>Results:</strong></em> The number of entries and time spent on the open arms of EPM decreased during the withdrawal state. Motor coordination and locomotor activity were significantly decreased. In the sub-acute study, clavulanic acid 80 mg/kg increased time spent and the number of entries to the open arms of EPM, in withdrawn animals. Both motor incoordination and locomotor activity reduction were normalized by clavulanic acid (10, 20, 40 and 80 mg/kg). Withdrawal-induced PTZ kindling seizure was also suppressed by all of the doses. MDA increased, while GSH decreased after withdrawal. Clavulanic acid attenuated such changes.<br /><em><strong>Conclusion:</strong></em> Clavulanic acid could prevent the development of alcohol withdrawal-induced anxiety and seizure. Alcohol withdrawal causes oxidative stress which can be prevented by clavulanic acid. |
topic |
alcohol withdrawal clavulanic acid elevated plus maze oxidative stress pentylenetetrazol rat |
url |
http://ijbms.mums.ac.ir/article_15478_662424c864ee8d42c41ca83ad818c32c.pdf |
work_keys_str_mv |
AT ehsanmohebi clavulanicacidimprovesethanolwithdrawalsymptomsinrats AT mehdimolavi clavulanicacidimprovesethanolwithdrawalsymptomsinrats AT mohammadmohammadzadeh clavulanicacidimprovesethanolwithdrawalsymptomsinrats AT hosseinhosseinzadeh clavulanicacidimprovesethanolwithdrawalsymptomsinrats AT baharehamin clavulanicacidimprovesethanolwithdrawalsymptomsinrats |
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