Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier Fly
Chitin and its deacetylated derivative chitosan are used for application in areas as an agriculture, biomedicine, cosmetic, food, textile and chelating agent for wastewater treatment. The current extraction of chitin is based on a chemical process using hydrochloric acid and sodium hydroxide. The ma...
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doaj-d0b5bf887d1d49b594638d78b4d8c3e42021-06-01T01:47:13ZengMDPI AGProcesses2227-97172021-05-01997697610.3390/pr9060976Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier FlyYin-Shen Lin0Shih-Hsiang Liang1Wen-Lin Lai2Ja-Xin Lee3Ya-Peng Wang4Yi-Tsz Liu5Szu-Han Wang6Meng-Hwan Lee7Division of Animal Technology, Animal Technology Research Center, Agricultural Technology Research Institute, No. 52 Kedong 2 Rd., Zhunan Township, Miaoli County 35053, TaiwanHsinchu Branch, Livestock Research Institute, Council of Agriculture, Miaoli County 36841, TaiwanDepartment of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, TaiwanHsinchu Branch, Livestock Research Institute, Council of Agriculture, Miaoli County 36841, TaiwanDivision of Animal Technology, Animal Technology Research Center, Agricultural Technology Research Institute, No. 52 Kedong 2 Rd., Zhunan Township, Miaoli County 35053, TaiwanDivision of Animal Technology, Animal Technology Research Center, Agricultural Technology Research Institute, No. 52 Kedong 2 Rd., Zhunan Township, Miaoli County 35053, TaiwanHsinchu Branch, Livestock Research Institute, Council of Agriculture, Miaoli County 36841, TaiwanDivision of Animal Technology, Animal Technology Research Center, Agricultural Technology Research Institute, No. 52 Kedong 2 Rd., Zhunan Township, Miaoli County 35053, TaiwanChitin and its deacetylated derivative chitosan are used for application in areas as an agriculture, biomedicine, cosmetic, food, textile and chelating agent for wastewater treatment. The current extraction of chitin is based on a chemical process using hydrochloric acid and sodium hydroxide. The main disadvantage of the process is the generation of large volumes of acid and alkaline effluents, which require further treatment before release. Chitin was extracted from spent pupal shell of black soldier fly (BSF) by the microbial fermentation method using <i>Bacillus lichenformis</i> A6. The recovery rate of chitin content by the microbial fermentation method was found to be about 12.4%. The structures of BSF chitin and chitosan were further characterized by FTIR, XRD, and SEM. Our results showed that the chitin obtained from BSF was observed in α form. The crystalline index values of chitin and chitosan are 52.8% and 55.4%, respectively. The surface morphology was examined by SEM, revealing nanofiber structures. The spent pupal shell of BSF may be used as alternative chitin sources for various technological purposes.https://www.mdpi.com/2227-9717/9/6/976ChitinChitosanBlack Soldier Fly<i>Hermetia illucens</i><i>Bacillus lichenformis</i> A6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yin-Shen Lin Shih-Hsiang Liang Wen-Lin Lai Ja-Xin Lee Ya-Peng Wang Yi-Tsz Liu Szu-Han Wang Meng-Hwan Lee |
spellingShingle |
Yin-Shen Lin Shih-Hsiang Liang Wen-Lin Lai Ja-Xin Lee Ya-Peng Wang Yi-Tsz Liu Szu-Han Wang Meng-Hwan Lee Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier Fly Processes Chitin Chitosan Black Soldier Fly <i>Hermetia illucens</i> <i>Bacillus lichenformis</i> A6 |
author_facet |
Yin-Shen Lin Shih-Hsiang Liang Wen-Lin Lai Ja-Xin Lee Ya-Peng Wang Yi-Tsz Liu Szu-Han Wang Meng-Hwan Lee |
author_sort |
Yin-Shen Lin |
title |
Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier Fly |
title_short |
Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier Fly |
title_full |
Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier Fly |
title_fullStr |
Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier Fly |
title_full_unstemmed |
Sustainable Extraction of Chitin from Spent Pupal Shell of Black Soldier Fly |
title_sort |
sustainable extraction of chitin from spent pupal shell of black soldier fly |
publisher |
MDPI AG |
series |
Processes |
issn |
2227-9717 |
publishDate |
2021-05-01 |
description |
Chitin and its deacetylated derivative chitosan are used for application in areas as an agriculture, biomedicine, cosmetic, food, textile and chelating agent for wastewater treatment. The current extraction of chitin is based on a chemical process using hydrochloric acid and sodium hydroxide. The main disadvantage of the process is the generation of large volumes of acid and alkaline effluents, which require further treatment before release. Chitin was extracted from spent pupal shell of black soldier fly (BSF) by the microbial fermentation method using <i>Bacillus lichenformis</i> A6. The recovery rate of chitin content by the microbial fermentation method was found to be about 12.4%. The structures of BSF chitin and chitosan were further characterized by FTIR, XRD, and SEM. Our results showed that the chitin obtained from BSF was observed in α form. The crystalline index values of chitin and chitosan are 52.8% and 55.4%, respectively. The surface morphology was examined by SEM, revealing nanofiber structures. The spent pupal shell of BSF may be used as alternative chitin sources for various technological purposes. |
topic |
Chitin Chitosan Black Soldier Fly <i>Hermetia illucens</i> <i>Bacillus lichenformis</i> A6 |
url |
https://www.mdpi.com/2227-9717/9/6/976 |
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