Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis
Hepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, m...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2021-01-01
|
Series: | Bioengineered |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/21655979.2021.1979439 |
id |
doaj-d0d1bddc05b7454abb2d8f76f7ab027e |
---|---|
record_format |
Article |
spelling |
doaj-d0d1bddc05b7454abb2d8f76f7ab027e2021-09-24T14:41:26ZengTaylor & Francis GroupBioengineered2165-59792165-59872021-01-011217165717710.1080/21655979.2021.19794391979439Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysisShangshang Hu0Jinyan Zhang1Xiaoyu Fang2Guoqing Guo3Jing Dai4Zhiyong Sheng5Dongdong Li6Jiasheng Chen7Li Zhang8Chuanmiao Liu9Yu Gao10Research Center of Clinical Laboratory Science, School of Laboratory Medicine, Bengbu Medical CollegeSchool of Life Science, Bengbu Medical CollegeFirst Affiliated Hospital of Bengbu Medical College, Bengbu Medical CollegeSchool of Life Science, Bengbu Medical CollegeSchool of Life Science, Bengbu Medical CollegeSchool of Life Science, Bengbu Medical CollegeFirst Affiliated Hospital of Bengbu Medical College, Bengbu Medical CollegeFirst Affiliated Hospital of Bengbu Medical College, Bengbu Medical CollegeFirst Affiliated Hospital of Bengbu Medical College, Bengbu Medical CollegeFirst Affiliated Hospital of Bengbu Medical College, Bengbu Medical CollegeSchool of Life Science, Bengbu Medical CollegeHepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, might play a role in suppressing tumor progression in a variety of tumors. The present study aims to examine miR-30 c effects in the development of HCC. The role of miR-30 c in HCC was comprehensively investigated by using bioinformatics and experiments in vitro. The multiple databases were combined to predict and screen the target genes and upstream lncRNAs of miR-30 c, and then constructed a competitive endogenous RNA (ceRNA) regulatory network with miR-30 c as the central miRNA. The miR-30 c-related ceRNA regulatory network was also initially validated in vitro. The results showed that miR-30 c over-expression could inhibit proliferation, migration, invasion, induce apoptosis, and increase G0/G1 phase ratio of HCC cells. Three miR-30 c upstream lncRNAs and 12 miR-30 c target genes were expressed in HCC cells with increased expression and poor prognosis, and a miR-30 C-related ceRNA regulatory network was constructed. This study verified miR-30 c as an inhibitory factor in the progression of HCC and performed analyses on the miR-30 c regulatory network, which might provide potential target information for HCC prognoses and therapies. However, further experiments in vivo and studies including clinical trials will be conducted to validate our results.http://dx.doi.org/10.1080/21655979.2021.1979439hsa-mir-30c-5phepatocellular carcinomamirnalncrnascerna regulatory networkinhibitory factor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shangshang Hu Jinyan Zhang Xiaoyu Fang Guoqing Guo Jing Dai Zhiyong Sheng Dongdong Li Jiasheng Chen Li Zhang Chuanmiao Liu Yu Gao |
spellingShingle |
Shangshang Hu Jinyan Zhang Xiaoyu Fang Guoqing Guo Jing Dai Zhiyong Sheng Dongdong Li Jiasheng Chen Li Zhang Chuanmiao Liu Yu Gao Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis Bioengineered hsa-mir-30c-5p hepatocellular carcinoma mirna lncrnas cerna regulatory network inhibitory factor |
author_facet |
Shangshang Hu Jinyan Zhang Xiaoyu Fang Guoqing Guo Jing Dai Zhiyong Sheng Dongdong Li Jiasheng Chen Li Zhang Chuanmiao Liu Yu Gao |
author_sort |
Shangshang Hu |
title |
Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis |
title_short |
Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis |
title_full |
Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis |
title_fullStr |
Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis |
title_full_unstemmed |
Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis |
title_sort |
identification of microrna hsa-mir-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis |
publisher |
Taylor & Francis Group |
series |
Bioengineered |
issn |
2165-5979 2165-5987 |
publishDate |
2021-01-01 |
description |
Hepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, might play a role in suppressing tumor progression in a variety of tumors. The present study aims to examine miR-30 c effects in the development of HCC. The role of miR-30 c in HCC was comprehensively investigated by using bioinformatics and experiments in vitro. The multiple databases were combined to predict and screen the target genes and upstream lncRNAs of miR-30 c, and then constructed a competitive endogenous RNA (ceRNA) regulatory network with miR-30 c as the central miRNA. The miR-30 c-related ceRNA regulatory network was also initially validated in vitro. The results showed that miR-30 c over-expression could inhibit proliferation, migration, invasion, induce apoptosis, and increase G0/G1 phase ratio of HCC cells. Three miR-30 c upstream lncRNAs and 12 miR-30 c target genes were expressed in HCC cells with increased expression and poor prognosis, and a miR-30 C-related ceRNA regulatory network was constructed. This study verified miR-30 c as an inhibitory factor in the progression of HCC and performed analyses on the miR-30 c regulatory network, which might provide potential target information for HCC prognoses and therapies. However, further experiments in vivo and studies including clinical trials will be conducted to validate our results. |
topic |
hsa-mir-30c-5p hepatocellular carcinoma mirna lncrnas cerna regulatory network inhibitory factor |
url |
http://dx.doi.org/10.1080/21655979.2021.1979439 |
work_keys_str_mv |
AT shangshanghu identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT jinyanzhang identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT xiaoyufang identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT guoqingguo identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT jingdai identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT zhiyongsheng identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT dongdongli identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT jiashengchen identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT lizhang identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT chuanmiaoliu identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis AT yugao identificationofmicrornahsamir30c5pasaninhibitoryfactorintheprogressionofhepatocellularcarcinomaandinvestigationofitsregulatorynetworkviacomprehensiveanalysis |
_version_ |
1717369693126262784 |