In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species
This study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige a...
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University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
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doaj-d0e440b8bf9b4c51bb89686ca86f637e2020-11-25T02:19:43ZengUniversity of Agricultural Sciences and Veterinary Medicine, Cluj-NapocaNotulae Scientia Biologicae2067-32052067-32642020-03-0112110.15835/nsb12110604In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding speciesMichael S. AKINROPO0Benjamin E. AYISIRE1Ejeoghene R. OGBIMI2Obafemi Awolowo University, Department of Botany, Ile-Ife, Osun StateObafemi Awolowo University, Department of Botany, Ile-Ife, Osun StateObafemi Awolowo University, Department of Botany, Ile-Ife, Osun StateThis study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination. The leaf explants did not respond to these treatments. The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal > stem > cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus. The highest callus production was observed on MS medium supplemented with 2.7 µM NAA + 2.2 µM BA and 5.4 µM NAA alone. Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 µM BA+2.7 µM NAA and 2.2µM BA+2.7 µM NAA respectively. On the basal medium, 10% of the excised shoots rooted successfully. Thus, this in vitro method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies. https://www.notulaebiologicae.ro/index.php/nsb/article/view/10604callus; caulogenesis; callogenesis; explants; multiple shoots |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael S. AKINROPO Benjamin E. AYISIRE Ejeoghene R. OGBIMI |
spellingShingle |
Michael S. AKINROPO Benjamin E. AYISIRE Ejeoghene R. OGBIMI In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species Notulae Scientia Biologicae callus; caulogenesis; callogenesis; explants; multiple shoots |
author_facet |
Michael S. AKINROPO Benjamin E. AYISIRE Ejeoghene R. OGBIMI |
author_sort |
Michael S. AKINROPO |
title |
In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species |
title_short |
In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species |
title_full |
In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species |
title_fullStr |
In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species |
title_full_unstemmed |
In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species |
title_sort |
in vitro callus and shoot regeneration in enterolobium cyclocarpum (jacq.) grised. - a fast timber yielding species |
publisher |
University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca |
series |
Notulae Scientia Biologicae |
issn |
2067-3205 2067-3264 |
publishDate |
2020-03-01 |
description |
This study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination. The leaf explants did not respond to these treatments. The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal > stem > cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus. The highest callus production was observed on MS medium supplemented with 2.7 µM NAA + 2.2 µM BA and 5.4 µM NAA alone. Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 µM BA+2.7 µM NAA and 2.2µM BA+2.7 µM NAA respectively. On the basal medium, 10% of the excised shoots rooted successfully. Thus, this in vitro method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies.
|
topic |
callus; caulogenesis; callogenesis; explants; multiple shoots |
url |
https://www.notulaebiologicae.ro/index.php/nsb/article/view/10604 |
work_keys_str_mv |
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