The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway
Background: Microorganisms and higher plants possess their own omega-3 and omega-6 polyunsaturated fatty acid (PUFAs) biosynthetic pathways. The n-6 fatty acid desaturase gene fad-2 codes for the n-6 desaturase enzyme that coverts oleic acid (OA 18:1 n-9) into linoleic acid (LA 18:2 n-6). The n-3 fa...
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Cell Physiol Biochem Press GmbH & Co KG
2013-08-01
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doaj-d0ee4d0bda3c4f5380b39c933c4f38022020-11-24T20:47:10ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-08-0132352353210.1159/000354456354456The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic PathwayYue ChenMei MeiPeng ZhangKuiying MaGuangqi SongXin MaTianchuang ZhaoBo TangHongsheng OuyangGuangpeng LiZiyi LiBackground: Microorganisms and higher plants possess their own omega-3 and omega-6 polyunsaturated fatty acid (PUFAs) biosynthetic pathways. The n-6 fatty acid desaturase gene fad-2 codes for the n-6 desaturase enzyme that coverts oleic acid (OA 18:1 n-9) into linoleic acid (LA 18:2 n-6). The n-3 fatty acid desaturase gene fat-1 codes for the n-3 desaturase enzyme that converts n-6 PUFAs into n-3 PUFAs. Mammals lack n-3 and n-6 desaturase enzymes; therefore, they must obtain their omega-3 and omega-6 fatty acids by consuming plants or seafood. The beneficial effects of n-3 and n-6 PUFAs on human development and cardiovascular health have been well documented. Methods: Here, we generated fat-1 and fad-2 transgenic mice by introducing mammal expression vectors containing the fat-1 and fad-2 genes via microinjection. Results: Seven transgenic mice were obtained that expressed functional n-3 and n-6 desaturase enzymes. Analysis of the fatty acid contents of transgenic mouse livers revealed that n-6 and n-3 PUFA levels were greatly increased in the transgenic mice compared to wild-type mice. The use ratios of n-9 PUFAs (18:1 n-9) and n-6 PUFAs were both greater in the transgenic mice than in the wild-type controls. Conclusion: These transgenic mice were capable of producing their own omega-3 and omega-6 fatty acids. They have the same fatty acid metabolic pathways as higher plants and microbes.http://www.karger.com/Article/FullText/354456Polyunsaturated fatty acids (PUFAs)Omega-3 PUFAsOmega-6 PUFAsFat-1Fad-2Transgenic mice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yue Chen Mei Mei Peng Zhang Kuiying Ma Guangqi Song Xin Ma Tianchuang Zhao Bo Tang Hongsheng Ouyang Guangpeng Li Ziyi Li |
spellingShingle |
Yue Chen Mei Mei Peng Zhang Kuiying Ma Guangqi Song Xin Ma Tianchuang Zhao Bo Tang Hongsheng Ouyang Guangpeng Li Ziyi Li The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway Cellular Physiology and Biochemistry Polyunsaturated fatty acids (PUFAs) Omega-3 PUFAs Omega-6 PUFAs Fat-1 Fad-2 Transgenic mice |
author_facet |
Yue Chen Mei Mei Peng Zhang Kuiying Ma Guangqi Song Xin Ma Tianchuang Zhao Bo Tang Hongsheng Ouyang Guangpeng Li Ziyi Li |
author_sort |
Yue Chen |
title |
The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway |
title_short |
The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway |
title_full |
The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway |
title_fullStr |
The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway |
title_full_unstemmed |
The Generation of Transgenic Mice with Fat1 and Fad2 Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway |
title_sort |
generation of transgenic mice with fat1 and fad2 genes that have their own polyunsaturated fatty acid biosynthetic pathway |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2013-08-01 |
description |
Background: Microorganisms and higher plants possess their own omega-3 and omega-6 polyunsaturated fatty acid (PUFAs) biosynthetic pathways. The n-6 fatty acid desaturase gene fad-2 codes for the n-6 desaturase enzyme that coverts oleic acid (OA 18:1 n-9) into linoleic acid (LA 18:2 n-6). The n-3 fatty acid desaturase gene fat-1 codes for the n-3 desaturase enzyme that converts n-6 PUFAs into n-3 PUFAs. Mammals lack n-3 and n-6 desaturase enzymes; therefore, they must obtain their omega-3 and omega-6 fatty acids by consuming plants or seafood. The beneficial effects of n-3 and n-6 PUFAs on human development and cardiovascular health have been well documented. Methods: Here, we generated fat-1 and fad-2 transgenic mice by introducing mammal expression vectors containing the fat-1 and fad-2 genes via microinjection. Results: Seven transgenic mice were obtained that expressed functional n-3 and n-6 desaturase enzymes. Analysis of the fatty acid contents of transgenic mouse livers revealed that n-6 and n-3 PUFA levels were greatly increased in the transgenic mice compared to wild-type mice. The use ratios of n-9 PUFAs (18:1 n-9) and n-6 PUFAs were both greater in the transgenic mice than in the wild-type controls. Conclusion: These transgenic mice were capable of producing their own omega-3 and omega-6 fatty acids. They have the same fatty acid metabolic pathways as higher plants and microbes. |
topic |
Polyunsaturated fatty acids (PUFAs) Omega-3 PUFAs Omega-6 PUFAs Fat-1 Fad-2 Transgenic mice |
url |
http://www.karger.com/Article/FullText/354456 |
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