Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural Area
Context: Methicillin resistant Staphylococcus aureus (MRSA) is an increasing problem in health care facilities. Rising colonization rates lead to increased infection rates in the community and in hospitals. MRSA infection in critically ill patients is associated with prolonged intensive care uni...
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doaj-d0f18b431b2345b582d2600eaf7e790f2020-11-25T03:14:02ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822013-10-0124182210.7860/NJLM/2013/6900:1996Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural AreaRajesh Bareja0Kanu Goel1Shinu Pottathil2 Vinod Kumar Narang3Varsha Ashok Singh4Prem Singh Grover5Assistant Professor, Department of Microbiology, M.M.I.M.S.R, Mullana, Ambala, Haryana – 133207, India.Assistant Professor, Department of Pathology, M.M.I.M.S.R, Mullana, Ambala, Haryana – 133207, India.Assistant Professor, Department of Microbiology, M.M.I.M.S.R, Mullana, Ambala, Haryana – 133207, India.Professor, Department of Microbiology, M.M.I.M.S.R, Mullana, Ambala, Haryana – 133207, India.Professor, Department of Microbiology, M.M.I.M.S.R, Mullana, Ambala, Haryana – 133207, IndiaProfessor, Department of Microbiology, M.M.I.M.S.R, Mullana, Ambala, Haryana – 133207, India.Context: Methicillin resistant Staphylococcus aureus (MRSA) is an increasing problem in health care facilities. Rising colonization rates lead to increased infection rates in the community and in hospitals. MRSA infection in critically ill patients is associated with prolonged intensive care unit stay, increased medical cost and high mortality. Aim: The prevalence and rate of acquisition of methicillinresistant S. aureus in nursing staff and patients admitted to the intensive care units (ICUs) were studied in order to estimate the possible risk for those, who are initially free of the organism, of acquiring MRSA infection while maintained in the ICU. Settings and Design: This was a cross sectional study conducted in the Department of Microbiology, M.M.I.M.S.R. Mullana, Ambala, Haryana, India. Material and Methods: A total of 400 specimens of hands, throat and nares from the fifty patients and fifty nursing staff of ICUs were subjected to bacteriological examination. All the specimens were processed on blood agar and MacConkey agar then incubated at 37°C in incubator for 24 hours. Suspicious colonies were identified as Staphylococcus aureus by various biochemical tests. Methicillin resistant was confirmed with cefoxitin disk (30μg) susceptibility test and Oxacillin Salt Agar method. Results: Of these, 234 (58.5%) isolates were found to be positive for MRSA. MRSA strains isolated from hands, throat and nares of patients and nursing staff were 74 (37%) and 78 (39%), 39 (39%) and 41 (41%), 2 (2%) and 0% respectively. Surgery ICU had the highest burden (30.25%) followed by Medicine ICU (17.75%) and Paediatric ICU (10.5%). Antibiotic sensitivity testing identified 31.63% MRSA strains to be resistant to vancomycin. Conclusion: The results indicate, screening of ICU staff and patients for methicillin resistant S. aureus colonization and infection, accompanied by antibiotic sensitivity testing of cultured isolates, is important to understand its epidemiology and to develop preventive measures and treatment strategieshttp://www.njlm.net/articles/PDF/1996/5-6900_E(_)_F(H)_PF1(SH)_PFA(H).pdfcefoxitin diskicumrsaoxacillin salt agar |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rajesh Bareja Kanu Goel Shinu Pottathil Vinod Kumar Narang Varsha Ashok Singh Prem Singh Grover |
spellingShingle |
Rajesh Bareja Kanu Goel Shinu Pottathil Vinod Kumar Narang Varsha Ashok Singh Prem Singh Grover Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural Area National Journal of Laboratory Medicine cefoxitin disk icu mrsa oxacillin salt agar |
author_facet |
Rajesh Bareja Kanu Goel Shinu Pottathil Vinod Kumar Narang Varsha Ashok Singh Prem Singh Grover |
author_sort |
Rajesh Bareja |
title |
Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural Area |
title_short |
Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural Area |
title_full |
Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural Area |
title_fullStr |
Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural Area |
title_full_unstemmed |
Evaluation of Methicillin Resistant Staphylococcus Aureus Colonization in Patients and Nursing Staff of Intensive Care Units of A Tertiary Care Hospital in A Rural Area |
title_sort |
evaluation of methicillin resistant staphylococcus aureus colonization in patients and nursing staff of intensive care units of a tertiary care hospital in a rural area |
publisher |
JCDR Research and Publications Pvt. Ltd. |
series |
National Journal of Laboratory Medicine |
issn |
2277-8551 2455-6882 |
publishDate |
2013-10-01 |
description |
Context: Methicillin resistant Staphylococcus aureus
(MRSA) is an increasing problem in health care facilities.
Rising colonization rates lead to increased infection rates in
the community and in hospitals. MRSA infection in critically
ill patients is associated with prolonged intensive care unit
stay, increased medical cost and high mortality.
Aim: The prevalence and rate of acquisition of methicillinresistant S. aureus in nursing staff and patients admitted to the
intensive care units (ICUs) were studied in order to estimate the
possible risk for those, who are initially free of the organism, of
acquiring MRSA infection while maintained in the ICU.
Settings and Design: This was a cross sectional study
conducted in the Department of Microbiology, M.M.I.M.S.R.
Mullana, Ambala, Haryana, India.
Material and Methods: A total of 400 specimens of hands,
throat and nares from the fifty patients and fifty nursing staff
of ICUs were subjected to bacteriological examination. All the
specimens were processed on blood agar and MacConkey
agar then incubated at 37°C in incubator for 24 hours.
Suspicious colonies were identified as Staphylococcus
aureus by various biochemical tests. Methicillin resistant
was confirmed with cefoxitin disk (30μg) susceptibility test
and Oxacillin Salt Agar method.
Results: Of these, 234 (58.5%) isolates were found to be
positive for MRSA. MRSA strains isolated from hands, throat
and nares of patients and nursing staff were 74 (37%) and 78
(39%), 39 (39%) and 41 (41%), 2 (2%) and 0% respectively.
Surgery ICU had the highest burden (30.25%) followed
by Medicine ICU (17.75%) and Paediatric ICU (10.5%).
Antibiotic sensitivity testing identified 31.63% MRSA strains
to be resistant to vancomycin.
Conclusion: The results indicate, screening of ICU staff
and patients for methicillin resistant S. aureus colonization
and infection, accompanied by antibiotic sensitivity
testing of cultured isolates, is important to understand its
epidemiology and to develop preventive measures and
treatment strategies |
topic |
cefoxitin disk icu mrsa oxacillin salt agar |
url |
http://www.njlm.net/articles/PDF/1996/5-6900_E(_)_F(H)_PF1(SH)_PFA(H).pdf |
work_keys_str_mv |
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