Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains Variabilidade fenotípica e antigênica de cepas variáveis de Mycoplasma gallisepticum
Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine F and Ts-11 strains were investigated by SDS-PAGE and serological methods (hemagglutination-inhibition and immunoblot assays). The SDS-PAGE system followed by densitometer analysis showed weak phenotypic variability between t...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2003-07-01
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Series: | Brazilian Journal of Microbiology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300011 |
Summary: | Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine F and Ts-11 strains were investigated by SDS-PAGE and serological methods (hemagglutination-inhibition and immunoblot assays). The SDS-PAGE system followed by densitometer analysis showed weak phenotypic variability between the strains, being the major difference close to the 75 kDa level where a prominent peptide band was detected only in the F vaccine strain. Polyclonal antibodies to the M. gallisepticum antigens produced in chickens were used in serological tests for this antigenic variability study. There were strong cross-reactions between the strains and homologous/ heterologous antibodies. The most evident characteristic was the specific response of the vaccine-type F polyclonal antiserum to the 75 kDa peptide band of the homologous strain.<br>Cepas vacinais de Mycoplasma gallisepticum, F e TS-11, foram examinadas quanto às suas variações fenotípicas e antigênicas, por SDS-PAGE e através de dois métodos sorológicos (inibição da hemaglutinação e imunoeletroforese). A análise densitométrica das bandas obtidas nos géis de poliacrilamida mostrou pequena variabilidade fenotípica entre as amostras, sendo a banda peptidica de 75 kDa detectada apenas na amostra vacinal F. Anticorpos policlonais produzidos em galinha foram utilizados nos ensaios sorológicos para estudar a variabilidade antigênica das amostras. Houve elevada reatividade cruzada entre as amostras e os anticorpos homólogos e heterólogos. A característica mais evidente foi a resposta específica da banda peptídica de 75 kDa da vacina F ao anticorpo homólogo. |
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ISSN: | 1517-8382 1678-4405 |