Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells
We previously reported that Runx2/miR-3960/miR-2861 regulatory feedback loop stimulates osteoblast differentiation. However, the effect of this feedback loop on the osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs) remains unclear. Our recent study showed that miR-2861 and miR-...
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doaj-d116e1c56e794c988f470ea3fa53566b2020-11-24T20:49:05ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/624037624037Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle CellsZhu-Ying Xia0Yin Hu1Ping-Li Xie2Si-Yuan Tang3Xiang-Hang Luo4Er-Yuan Liao5Fei Chen6Hui Xie7Institute of Endocrinology and Metabolism, Second Xiang-Ya Hospital of Central South University, Changsha, Hunan 410011, ChinaInstitute of Endocrinology and Metabolism, Second Xiang-Ya Hospital of Central South University, Changsha, Hunan 410011, ChinaDepartment of Forensic Medicine, School of Basic Medical Science of Central South University, Changsha, Hunan 410013, ChinaSchool of Nursing of Central South University, Changsha, Hunan 410013, ChinaInstitute of Endocrinology and Metabolism, Second Xiang-Ya Hospital of Central South University, Changsha, Hunan 410011, ChinaInstitute of Endocrinology and Metabolism, Second Xiang-Ya Hospital of Central South University, Changsha, Hunan 410011, ChinaSpine Surgery Department, Second Xiang-Ya Hospital of Central South University, Changsha, Hunan 410011, ChinaInstitute of Endocrinology and Metabolism, Second Xiang-Ya Hospital of Central South University, Changsha, Hunan 410011, ChinaWe previously reported that Runx2/miR-3960/miR-2861 regulatory feedback loop stimulates osteoblast differentiation. However, the effect of this feedback loop on the osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs) remains unclear. Our recent study showed that miR-2861 and miR-3960 expression increases significantly during β-glycerophosphate-induced osteogenic transdifferentiation of VSMCs. Overexpression of miR-2861 or miR-3960 in VSMCs enhances β-glycerophosphate-induced osteoblastogenesis, whereas inhibition of miR-2861 or miR-3960 expression attenuates it. MiR-2861 or miR-3960 promotes osteogenic transdifferentiation of VSMCs by targeting histone deacetylase 5 or Homeobox A2, respectively, resulting in increased runt-related transcription factor 2 (Runx2) protein production. Furthermore, overexpression of Runx2 induces miR-2861 and miR-3960 transcription, and knockdown of Runx2 attenuates β-glycerophosphate-induced miR-2861 and miR-3960 transcription in VSMCs. Thus, our data show that Runx2/miR-3960/miR-2861 positive feedback loop plays an important role in osteogenic transdifferentiation of VSMCs and contributes to vascular calcification.http://dx.doi.org/10.1155/2015/624037 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhu-Ying Xia Yin Hu Ping-Li Xie Si-Yuan Tang Xiang-Hang Luo Er-Yuan Liao Fei Chen Hui Xie |
spellingShingle |
Zhu-Ying Xia Yin Hu Ping-Li Xie Si-Yuan Tang Xiang-Hang Luo Er-Yuan Liao Fei Chen Hui Xie Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells BioMed Research International |
author_facet |
Zhu-Ying Xia Yin Hu Ping-Li Xie Si-Yuan Tang Xiang-Hang Luo Er-Yuan Liao Fei Chen Hui Xie |
author_sort |
Zhu-Ying Xia |
title |
Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells |
title_short |
Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells |
title_full |
Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells |
title_fullStr |
Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells |
title_full_unstemmed |
Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells |
title_sort |
runx2/mir-3960/mir-2861 positive feedback loop is responsible for osteogenic transdifferentiation of vascular smooth muscle cells |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
We previously reported that Runx2/miR-3960/miR-2861 regulatory feedback loop stimulates osteoblast differentiation. However, the effect of this feedback loop on the osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs) remains unclear. Our recent study showed that miR-2861 and miR-3960 expression increases significantly during β-glycerophosphate-induced osteogenic transdifferentiation of VSMCs. Overexpression of miR-2861 or miR-3960 in VSMCs enhances β-glycerophosphate-induced osteoblastogenesis, whereas inhibition of miR-2861 or miR-3960 expression attenuates it. MiR-2861 or miR-3960 promotes osteogenic transdifferentiation of VSMCs by targeting histone deacetylase 5 or Homeobox A2, respectively, resulting in increased runt-related transcription factor 2 (Runx2) protein production. Furthermore, overexpression of Runx2 induces miR-2861 and miR-3960 transcription, and knockdown of Runx2 attenuates β-glycerophosphate-induced miR-2861 and miR-3960 transcription in VSMCs. Thus, our data show that Runx2/miR-3960/miR-2861 positive feedback loop plays an important role in osteogenic transdifferentiation of VSMCs and contributes to vascular calcification. |
url |
http://dx.doi.org/10.1155/2015/624037 |
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