Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver
Chronic hepatitises of various etiologies are widespread liver diseases in humans. Their final stage, liver cirrhosis (LC), is considered to be one of the main causes of hepatocellular carcinoma (HCC). About 80–90% of all HCC cases develop in LC patients, which suggests that cirrhotic conditions pla...
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doaj-d127000fac44416da447083bebef88352021-04-21T23:07:12ZengMDPI AGCells2073-44092021-04-011097697610.3390/cells10050976Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic LiverNatalia N. Bezborodkina0Sergey V. Okovityi1Boris N. Kudryavtsev2Zoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, 199034 St. Petersburg, RussiaDepartment of Pharmacology and Clinical Pharmacology, Saint Petersburg State Chemical Pharmaceutical University, 197022 St. Petersburg, RussiaScientific-Clinical Centre, Pavlov First Saint Petersburg State Medical University, L’va Tolstogo str. 6-8, 197022 St. Petersburg, RussiaChronic hepatitises of various etiologies are widespread liver diseases in humans. Their final stage, liver cirrhosis (LC), is considered to be one of the main causes of hepatocellular carcinoma (HCC). About 80–90% of all HCC cases develop in LC patients, which suggests that cirrhotic conditions play a crucial role in the process of hepatocarcinogenesis. Carbohydrate metabolism in LC undergoes profound disturbances characterized by altered glycogen metabolism. Unfortunately, data on the glycogen content in LC are few and contradictory. In this study, the material was obtained from liver biopsies of patients with LC of viral and alcohol etiology and from the liver tissue of rats with CCl<sub>4</sub>-induced LC. The activity of glycogen phosphorylase (GP), glycogen synthase (GS), and glucose-6-phosphatase (G6Pase) was investigated in human and rat liver tissue by biochemical methods. Total glycogen and its labile and stable fractions were measured in isolated individual hepatocytes, using the cytofluorometry technique of PAS reaction<i> </i>in situ. The development of LC in human and rat liver was accompanied by an increase in fibrous tissue (20- and 8.8-fold), an increase in the dry mass of hepatocytes (by 25.6% and 23.7%), and a decrease in the number of hepatocytes (by 50% and 28%), respectively. The rearrangement of the liver parenchyma was combined with changes in glycogen metabolism. The present study showed a significant increase in the glycogen content in the hepatocytes of the human and the rat cirrhotic liver, by 255% and 210%, respectively. An increased glycogen content in cells of the cirrhotic liver can be explained by a decrease in glycogenolysis due to a decreased activity of G6Pase and GP.https://www.mdpi.com/2073-4409/10/5/976liver cirrhosisglycogenhepatocytesglycogen phosphorylaseglycogen synthaseglucose-6-phosphatase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natalia N. Bezborodkina Sergey V. Okovityi Boris N. Kudryavtsev |
spellingShingle |
Natalia N. Bezborodkina Sergey V. Okovityi Boris N. Kudryavtsev Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver Cells liver cirrhosis glycogen hepatocytes glycogen phosphorylase glycogen synthase glucose-6-phosphatase |
author_facet |
Natalia N. Bezborodkina Sergey V. Okovityi Boris N. Kudryavtsev |
author_sort |
Natalia N. Bezborodkina |
title |
Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver |
title_short |
Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver |
title_full |
Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver |
title_fullStr |
Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver |
title_full_unstemmed |
Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver |
title_sort |
postprandial glycogen content is increased in the hepatocytes of human and rat cirrhotic liver |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-04-01 |
description |
Chronic hepatitises of various etiologies are widespread liver diseases in humans. Their final stage, liver cirrhosis (LC), is considered to be one of the main causes of hepatocellular carcinoma (HCC). About 80–90% of all HCC cases develop in LC patients, which suggests that cirrhotic conditions play a crucial role in the process of hepatocarcinogenesis. Carbohydrate metabolism in LC undergoes profound disturbances characterized by altered glycogen metabolism. Unfortunately, data on the glycogen content in LC are few and contradictory. In this study, the material was obtained from liver biopsies of patients with LC of viral and alcohol etiology and from the liver tissue of rats with CCl<sub>4</sub>-induced LC. The activity of glycogen phosphorylase (GP), glycogen synthase (GS), and glucose-6-phosphatase (G6Pase) was investigated in human and rat liver tissue by biochemical methods. Total glycogen and its labile and stable fractions were measured in isolated individual hepatocytes, using the cytofluorometry technique of PAS reaction<i> </i>in situ. The development of LC in human and rat liver was accompanied by an increase in fibrous tissue (20- and 8.8-fold), an increase in the dry mass of hepatocytes (by 25.6% and 23.7%), and a decrease in the number of hepatocytes (by 50% and 28%), respectively. The rearrangement of the liver parenchyma was combined with changes in glycogen metabolism. The present study showed a significant increase in the glycogen content in the hepatocytes of the human and the rat cirrhotic liver, by 255% and 210%, respectively. An increased glycogen content in cells of the cirrhotic liver can be explained by a decrease in glycogenolysis due to a decreased activity of G6Pase and GP. |
topic |
liver cirrhosis glycogen hepatocytes glycogen phosphorylase glycogen synthase glucose-6-phosphatase |
url |
https://www.mdpi.com/2073-4409/10/5/976 |
work_keys_str_mv |
AT natalianbezborodkina postprandialglycogencontentisincreasedinthehepatocytesofhumanandratcirrhoticliver AT sergeyvokovityi postprandialglycogencontentisincreasedinthehepatocytesofhumanandratcirrhoticliver AT borisnkudryavtsev postprandialglycogencontentisincreasedinthehepatocytesofhumanandratcirrhoticliver |
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