Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer
The human microbiota comprises trillions of microbes, and the relationship between cancer and microbiota is very complex. The impact of fecal microbiota alterations on colorectal cancer (CRC) pathogenesis is emerging. This study analyzed changes in the microbial composition in CRC subjects with both...
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2021-09-01
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doaj-d14d1dfcec404fc98fec3a9ebd5b34372021-09-14T06:14:03ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-09-011110.3389/fonc.2021.650026650026Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal CancerJihye Park0Nam-Eun Kim1Hyuk Yoon2Cheol Min Shin3Nayoung Kim4Dong Ho Lee5Jae Yong Park6Chang Hwan Choi7Jae Gyu Kim8Yoon-Keun Kim9Tae-Seop Shin10Jinho Yang11Young Soo Park12Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South KoreaDepartment of Public Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, South KoreaDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, South KoreaDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, South KoreaDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, South KoreaDepartment of Internal Medicine, Chung-Ang University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Chung-Ang University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Chung-Ang University College of Medicine, Seoul, South KoreaR&D Center, Institute of MD Healthcare Inc., Seoul, South KoreaR&D Center, Institute of MD Healthcare Inc., Seoul, South KoreaR&D Center, Institute of MD Healthcare Inc., Seoul, South KoreaDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, South KoreaThe human microbiota comprises trillions of microbes, and the relationship between cancer and microbiota is very complex. The impact of fecal microbiota alterations on colorectal cancer (CRC) pathogenesis is emerging. This study analyzed changes in the microbial composition in CRC subjects with both fecal microbiota and gut microbe-derived extracellular vesicles (EVs). From August 2017 to August 2018, 70 CRC patients and 158 control subjects were enrolled in the study. Metagenomic profiling of fecal microbiota and gut microbe-derived EVs in stool was performed using 16S ribosomal DNA sequencing. Relative abundance, evenness, and diversity in both the gut microbiota and gut microbe-derived EVs were analyzed. Additionally, microbial composition changes according to the stage and location of CRC were analyzed. Microbial composition was significantly changed in CRC subjects compared to control subjects, with evenness and diversity significantly lower in the fecal microbiota of CRC subjects. Gut microbe-derived EVs of stool demonstrated significant differences in the microbial composition, evenness, and diversity in CRC subjects compared to the control subjects. Additionally, microbial composition, evenness, and diversity significantly changed in late CRC subjects compared to early CRC subjects with both fecal microbiota and gut microbe-derived EVs. Alistipes-derived EVs could be novel biomarkers for diagnosing CRC and predicting CRC stages. Ruminococcus 2-derived EVs significantly decreased in distal CRC subjects than in proximal CRC subjects. Gut microbe-derived EVs in CRC had a distinct microbial composition compared to the controls. Profiling of microbe-derived EVs may offer a novel biomarker for detecting and predicting CRC prognosis.https://www.frontiersin.org/articles/10.3389/fonc.2021.650026/fullmicrobiomemetagenomegut microbe-derived extracellular vesiclescolorectal cancercancer stage |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jihye Park Nam-Eun Kim Hyuk Yoon Cheol Min Shin Nayoung Kim Dong Ho Lee Jae Yong Park Chang Hwan Choi Jae Gyu Kim Yoon-Keun Kim Tae-Seop Shin Jinho Yang Young Soo Park |
spellingShingle |
Jihye Park Nam-Eun Kim Hyuk Yoon Cheol Min Shin Nayoung Kim Dong Ho Lee Jae Yong Park Chang Hwan Choi Jae Gyu Kim Yoon-Keun Kim Tae-Seop Shin Jinho Yang Young Soo Park Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer Frontiers in Oncology microbiome metagenome gut microbe-derived extracellular vesicles colorectal cancer cancer stage |
author_facet |
Jihye Park Nam-Eun Kim Hyuk Yoon Cheol Min Shin Nayoung Kim Dong Ho Lee Jae Yong Park Chang Hwan Choi Jae Gyu Kim Yoon-Keun Kim Tae-Seop Shin Jinho Yang Young Soo Park |
author_sort |
Jihye Park |
title |
Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer |
title_short |
Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer |
title_full |
Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer |
title_fullStr |
Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer |
title_full_unstemmed |
Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer |
title_sort |
fecal microbiota and gut microbe-derived extracellular vesicles in colorectal cancer |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2021-09-01 |
description |
The human microbiota comprises trillions of microbes, and the relationship between cancer and microbiota is very complex. The impact of fecal microbiota alterations on colorectal cancer (CRC) pathogenesis is emerging. This study analyzed changes in the microbial composition in CRC subjects with both fecal microbiota and gut microbe-derived extracellular vesicles (EVs). From August 2017 to August 2018, 70 CRC patients and 158 control subjects were enrolled in the study. Metagenomic profiling of fecal microbiota and gut microbe-derived EVs in stool was performed using 16S ribosomal DNA sequencing. Relative abundance, evenness, and diversity in both the gut microbiota and gut microbe-derived EVs were analyzed. Additionally, microbial composition changes according to the stage and location of CRC were analyzed. Microbial composition was significantly changed in CRC subjects compared to control subjects, with evenness and diversity significantly lower in the fecal microbiota of CRC subjects. Gut microbe-derived EVs of stool demonstrated significant differences in the microbial composition, evenness, and diversity in CRC subjects compared to the control subjects. Additionally, microbial composition, evenness, and diversity significantly changed in late CRC subjects compared to early CRC subjects with both fecal microbiota and gut microbe-derived EVs. Alistipes-derived EVs could be novel biomarkers for diagnosing CRC and predicting CRC stages. Ruminococcus 2-derived EVs significantly decreased in distal CRC subjects than in proximal CRC subjects. Gut microbe-derived EVs in CRC had a distinct microbial composition compared to the controls. Profiling of microbe-derived EVs may offer a novel biomarker for detecting and predicting CRC prognosis. |
topic |
microbiome metagenome gut microbe-derived extracellular vesicles colorectal cancer cancer stage |
url |
https://www.frontiersin.org/articles/10.3389/fonc.2021.650026/full |
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