DETECTION OF HUMAN BLOODSTAINS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

• Main Authors: GHOLAM-HOSSEIN EDRISSIAN, ABBAS HAFIZI
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 1982-07-01
• Series: Acta Medica Iranica
• Online Access: http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/5107.pdf&manuscript_id=5107

The microplate method of e nzyme-l i nke d i rnmuno s o r be n t a s s ay (ELISA) , usi ng alkaline phosphatase anti-human I gG conj ugate , was modified and appl ied in t he dete c t i on of the human b lood specimens among the blood samples prep a r ed fr om human and laborat or y anima l s i n t he form of small dr ied b loodst a i ns on f ilt e r paper . The modi f i e d ELISA t e chnique wa s compa red wit h t he agar double d i f f us ion me t hod of prec ipitin test ."nThe results of this primary study showed t ha t the ELISA i n compare to gel diffusi o n t e st is sensitive and specific. It is also enough repr oducible and practical t o be consider as a competent technique for i de nt i f i c ation o f human bloodstains in medicolegal laboratories .