miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9
Objective: To investigate the role of microRNA-31 (miR-31) in osteosarcoma and the molecular mechanism of miR-31 in proliferation and apoptosis of osteosarcoma. Methods: real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-31 in human osteosarcoma. Pearson 's chisqua...
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doaj-d160e091270545e3af67b3d4578315d32020-11-25T03:48:49ZengEditorial Board of Journal of Hainan Medical UniversityJournal of Hainan Medical University1007-12371007-12372020-07-0126132733miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9Le-Meng Chao0Jian-Feng Liu1Inner Mongolia People’s Hospital, Spine Surgery, Hohhot, Inner Mongolia, 010000Inner Mongolia People’s Hospital, Spine Surgery, Hohhot, Inner Mongolia, 010000Objective: To investigate the role of microRNA-31 (miR-31) in osteosarcoma and the molecular mechanism of miR-31 in proliferation and apoptosis of osteosarcoma. Methods: real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-31 in human osteosarcoma. Pearson 's chisquared was applied to detect the correlation between miR-31 and clinicopathological features. CCK-8 and ANNEXIN-FITC/PI double staining were used to detect the proliferation and apoptosis of osteosarcoma cells. Results: The results of qRT-PCR showed that the expression of miR-31 was down-regulated in osteosarcoma tissues compared with matched precancerous bone tissues. The level of miR-31 in osteosarcoma tissues with high-grade malignancy and poor overall survival was even lower. CCK-8 and Annexin-FITC/PI double staining assays showed that miR-31 inhibited proliferation and promoted apoptosis of osteosarcoma cells. According to luciferase assay, miR-31 inhibited PAX9 expression directly. And PAX9 promoted proliferation and inhibited apoptosis of osteosarcoma cells. Conclusion: miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells by targeting PAX9. miR-31 and PAX9 can be used as therapeutic targets for osteosarcoma.http://www.hnykdxxb.com/PDF/202013/06.pdfosteosarcomaproliferationapoptosismir-31pax9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Le-Meng Chao Jian-Feng Liu |
spellingShingle |
Le-Meng Chao Jian-Feng Liu miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9 Journal of Hainan Medical University osteosarcoma proliferation apoptosis mir-31 pax9 |
author_facet |
Le-Meng Chao Jian-Feng Liu |
author_sort |
Le-Meng Chao |
title |
miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9 |
title_short |
miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9 |
title_full |
miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9 |
title_fullStr |
miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9 |
title_full_unstemmed |
miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through PAX9 |
title_sort |
mir-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells through pax9 |
publisher |
Editorial Board of Journal of Hainan Medical University |
series |
Journal of Hainan Medical University |
issn |
1007-1237 1007-1237 |
publishDate |
2020-07-01 |
description |
Objective: To investigate the role of microRNA-31 (miR-31) in osteosarcoma and the
molecular mechanism of miR-31 in proliferation and apoptosis of osteosarcoma. Methods:
real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-31 in human
osteosarcoma. Pearson 's chisquared was applied to detect the correlation between miR-31 and
clinicopathological features. CCK-8 and ANNEXIN-FITC/PI double staining were used to
detect the proliferation and apoptosis of osteosarcoma cells. Results: The results of qRT-PCR
showed that the expression of miR-31 was down-regulated in osteosarcoma tissues compared
with matched precancerous bone tissues. The level of miR-31 in osteosarcoma tissues with
high-grade malignancy and poor overall survival was even lower. CCK-8 and Annexin-FITC/PI
double staining assays showed that miR-31 inhibited proliferation and promoted apoptosis of
osteosarcoma cells. According to luciferase assay, miR-31 inhibited PAX9 expression directly.
And PAX9 promoted proliferation and inhibited apoptosis of osteosarcoma cells. Conclusion:
miR-31 inhibits proliferation and promotes apoptosis of osteosarcoma cells by targeting PAX9.
miR-31 and PAX9 can be used as therapeutic targets for osteosarcoma. |
topic |
osteosarcoma proliferation apoptosis mir-31 pax9 |
url |
http://www.hnykdxxb.com/PDF/202013/06.pdf |
work_keys_str_mv |
AT lemengchao mir31inhibitsproliferationandpromotesapoptosisofosteosarcomacellsthroughpax9 AT jianfengliu mir31inhibitsproliferationandpromotesapoptosisofosteosarcomacellsthroughpax9 |
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1724497029297602560 |