Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies
The insect mushroom body (MB) is a conserved brain structure that plays key roles in a diverse array of behaviors. The Drosophila melanogaster MB is the primary invertebrate model of neural circuits related to memory formation and storage, and its development, morphology, wiring, and function has be...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Oxford University Press
2019-01-01
|
Series: | G3: Genes, Genomes, Genetics |
Subjects: | |
Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.118.200726 |
id |
doaj-d1629486e822402a8cc1d9509d43fffd |
---|---|
record_format |
Article |
spelling |
doaj-d1629486e822402a8cc1d9509d43fffd2021-07-02T15:16:38ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362019-01-0191819410.1534/g3.118.2007268Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom BodiesMeng-Fu Maxwell ShihFred Pejman DavisGilbert Lee HenryJosh DubnauThe insect mushroom body (MB) is a conserved brain structure that plays key roles in a diverse array of behaviors. The Drosophila melanogaster MB is the primary invertebrate model of neural circuits related to memory formation and storage, and its development, morphology, wiring, and function has been extensively studied. MBs consist of intrinsic Kenyon Cells that are divided into three major neuron classes (γ, α′/β′ and α/β) and 7 cell subtypes (γd, γm, α′/β′ap, α′/β′m, α/βp, α/βs and α/βc) based on their birth order, morphology, and connectivity. These subtypes play distinct roles in memory processing, however the underlying transcriptional differences are unknown. Here, we used RNA sequencing (RNA-seq) to profile the nuclear transcriptomes of each MB neuronal cell subtypes. We identified 350 MB class- or subtype-specific genes, including the widely used α/β class marker Fas2 and the α′/β′ class marker trio. Immunostaining corroborates the RNA-seq measurements at the protein level for several cases. Importantly, our data provide a full accounting of the neurotransmitter receptors, transporters, neurotransmitter biosynthetic enzymes, neuropeptides, and neuropeptide receptors expressed within each of these cell types. This high-quality, cell type-level transcriptome catalog for the Drosophila MB provides a valuable resource for the fly neuroscience community.http://g3journal.org/lookup/doi/10.1534/g3.118.200726Drosophilamushroom bodytranscriptomeRNA-seq |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meng-Fu Maxwell Shih Fred Pejman Davis Gilbert Lee Henry Josh Dubnau |
spellingShingle |
Meng-Fu Maxwell Shih Fred Pejman Davis Gilbert Lee Henry Josh Dubnau Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies G3: Genes, Genomes, Genetics Drosophila mushroom body transcriptome RNA-seq |
author_facet |
Meng-Fu Maxwell Shih Fred Pejman Davis Gilbert Lee Henry Josh Dubnau |
author_sort |
Meng-Fu Maxwell Shih |
title |
Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies |
title_short |
Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies |
title_full |
Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies |
title_fullStr |
Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies |
title_full_unstemmed |
Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies |
title_sort |
nuclear transcriptomes of the seven neuronal cell types that constitute the drosophila mushroom bodies |
publisher |
Oxford University Press |
series |
G3: Genes, Genomes, Genetics |
issn |
2160-1836 |
publishDate |
2019-01-01 |
description |
The insect mushroom body (MB) is a conserved brain structure that plays key roles in a diverse array of behaviors. The Drosophila melanogaster MB is the primary invertebrate model of neural circuits related to memory formation and storage, and its development, morphology, wiring, and function has been extensively studied. MBs consist of intrinsic Kenyon Cells that are divided into three major neuron classes (γ, α′/β′ and α/β) and 7 cell subtypes (γd, γm, α′/β′ap, α′/β′m, α/βp, α/βs and α/βc) based on their birth order, morphology, and connectivity. These subtypes play distinct roles in memory processing, however the underlying transcriptional differences are unknown. Here, we used RNA sequencing (RNA-seq) to profile the nuclear transcriptomes of each MB neuronal cell subtypes. We identified 350 MB class- or subtype-specific genes, including the widely used α/β class marker Fas2 and the α′/β′ class marker trio. Immunostaining corroborates the RNA-seq measurements at the protein level for several cases. Importantly, our data provide a full accounting of the neurotransmitter receptors, transporters, neurotransmitter biosynthetic enzymes, neuropeptides, and neuropeptide receptors expressed within each of these cell types. This high-quality, cell type-level transcriptome catalog for the Drosophila MB provides a valuable resource for the fly neuroscience community. |
topic |
Drosophila mushroom body transcriptome RNA-seq |
url |
http://g3journal.org/lookup/doi/10.1534/g3.118.200726 |
work_keys_str_mv |
AT mengfumaxwellshih nucleartranscriptomesofthesevenneuronalcelltypesthatconstitutethedrosophilamushroombodies AT fredpejmandavis nucleartranscriptomesofthesevenneuronalcelltypesthatconstitutethedrosophilamushroombodies AT gilbertleehenry nucleartranscriptomesofthesevenneuronalcelltypesthatconstitutethedrosophilamushroombodies AT joshdubnau nucleartranscriptomesofthesevenneuronalcelltypesthatconstitutethedrosophilamushroombodies |
_version_ |
1721327401666871296 |