Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation)
The convergence of distinct lineages upon interspecific hybridisation, including when accompanied by increases in ploidy (allopolyploidy), is a driving force in the origin of many plant species. In plant breeding too, both interspecific hybridisation and allopolyploidy are important because they fac...
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doaj-d1768cff455643a687dc3cf3a819907f2020-11-24T23:40:45ZengMDPI AGGenes2073-44252010-07-011216619210.3390/genes1020166Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation)Michael ChesterAndrew R. LeitchPamela S. SoltisDouglas E. SoltisThe convergence of distinct lineages upon interspecific hybridisation, including when accompanied by increases in ploidy (allopolyploidy), is a driving force in the origin of many plant species. In plant breeding too, both interspecific hybridisation and allopolyploidy are important because they facilitate introgression of alien DNA into breeding lines enabling the introduction of novel characters. Here we review how fluorescence in situ hybridisation (FISH) and genomic in situ hybridisation (GISH) have been applied to: 1) studies of interspecific hybridisation and polyploidy in nature, 2) analyses of phylogenetic relationships between species, 3) genetic mapping and 4) analysis of plant breeding materials. We also review how FISH is poised to take advantage of nextgeneration sequencing (NGS) technologies, helping the rapid characterisation of the repetitive fractions of a genome in natural populations and agricultural plants. http://www.mdpi.com/2073-4425/1/2/166/cytogeneticsISHpolyploidy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael Chester Andrew R. Leitch Pamela S. Soltis Douglas E. Soltis |
spellingShingle |
Michael Chester Andrew R. Leitch Pamela S. Soltis Douglas E. Soltis Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation) Genes cytogenetics ISH polyploidy |
author_facet |
Michael Chester Andrew R. Leitch Pamela S. Soltis Douglas E. Soltis |
author_sort |
Michael Chester |
title |
Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation) |
title_short |
Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation) |
title_full |
Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation) |
title_fullStr |
Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation) |
title_full_unstemmed |
Review of the Application of Modern Cytogenetic Methods (FISH/GISH) to the Study of Reticulation (Polyploidy/Hybridisation) |
title_sort |
review of the application of modern cytogenetic methods (fish/gish) to the study of reticulation (polyploidy/hybridisation) |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2010-07-01 |
description |
The convergence of distinct lineages upon interspecific hybridisation, including when accompanied by increases in ploidy (allopolyploidy), is a driving force in the origin of many plant species. In plant breeding too, both interspecific hybridisation and allopolyploidy are important because they facilitate introgression of alien DNA into breeding lines enabling the introduction of novel characters. Here we review how fluorescence in situ hybridisation (FISH) and genomic in situ hybridisation (GISH) have been applied to: 1) studies of interspecific hybridisation and polyploidy in nature, 2) analyses of phylogenetic relationships between species, 3) genetic mapping and 4) analysis of plant breeding materials. We also review how FISH is poised to take advantage of nextgeneration sequencing (NGS) technologies, helping the rapid characterisation of the repetitive fractions of a genome in natural populations and agricultural plants. |
topic |
cytogenetics ISH polyploidy |
url |
http://www.mdpi.com/2073-4425/1/2/166/ |
work_keys_str_mv |
AT michaelchester reviewoftheapplicationofmoderncytogeneticmethodsfishgishtothestudyofreticulationpolyploidyhybridisation AT andrewrleitch reviewoftheapplicationofmoderncytogeneticmethodsfishgishtothestudyofreticulationpolyploidyhybridisation AT pamelassoltis reviewoftheapplicationofmoderncytogeneticmethodsfishgishtothestudyofreticulationpolyploidyhybridisation AT douglasesoltis reviewoftheapplicationofmoderncytogeneticmethodsfishgishtothestudyofreticulationpolyploidyhybridisation |
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1725509204987346944 |