Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides
Abstract The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owi...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-08-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-07516-x |
id |
doaj-d18ecd060b114aa492c4c68513b535e2 |
---|---|
record_format |
Article |
spelling |
doaj-d18ecd060b114aa492c4c68513b535e22020-12-08T02:23:30ZengNature Publishing GroupScientific Reports2045-23222017-08-017111110.1038/s41598-017-07516-xPolymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptidesFenglong Jiao0Fangyuan Gao1Heping Wang2Yulin Deng3Yangjun Zhang4Xiaohong Qian5Yukui Zhang6School of Life Science and Technology, Beijing Institute of TechnologyState Key Laboratory of Proteomics, National Center for Protein Science Beijing, Beijing Institute of Radiation MedicineState Key Laboratory of Proteomics, National Center for Protein Science Beijing, Beijing Institute of Radiation MedicineSchool of Life Science and Technology, Beijing Institute of TechnologyState Key Laboratory of Proteomics, National Center for Protein Science Beijing, Beijing Institute of Radiation MedicineState Key Laboratory of Proteomics, National Center for Protein Science Beijing, Beijing Institute of Radiation MedicineNational Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of SciencesAbstract The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing to the excellent hydrophilicity and strong electrostatic interaction toward glycopeptides of the polymerized hydrophilic ionic liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), the synthesized Fe3O4@MPS@PMAC nanoparticles exhibited outstanding performance in glycopeptide enrichment with high detection sensitivity (10 fmol), large binding capacity (100 μg mg−1) and satisfied enrichment recovery (approximately 82%). Furthermore, the newly developed Fe3O4@MPS@PMAC nanoparticles were applied for the glycopeptide enrichment of HeLa exosome proteins. A total of 1274 glycopeptides from 536 glycoproteins were identified in three replicate analyses of 50 μg of HeLa exosome proteins. These results demonstrate the potential of Fe3O4@MPS@PMAC nanoparticles for both glycoproteomic analysis and exosome research.https://doi.org/10.1038/s41598-017-07516-x |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fenglong Jiao Fangyuan Gao Heping Wang Yulin Deng Yangjun Zhang Xiaohong Qian Yukui Zhang |
spellingShingle |
Fenglong Jiao Fangyuan Gao Heping Wang Yulin Deng Yangjun Zhang Xiaohong Qian Yukui Zhang Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides Scientific Reports |
author_facet |
Fenglong Jiao Fangyuan Gao Heping Wang Yulin Deng Yangjun Zhang Xiaohong Qian Yukui Zhang |
author_sort |
Fenglong Jiao |
title |
Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides |
title_short |
Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides |
title_full |
Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides |
title_fullStr |
Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides |
title_full_unstemmed |
Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides |
title_sort |
polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of n-linked glycopeptides |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing to the excellent hydrophilicity and strong electrostatic interaction toward glycopeptides of the polymerized hydrophilic ionic liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), the synthesized Fe3O4@MPS@PMAC nanoparticles exhibited outstanding performance in glycopeptide enrichment with high detection sensitivity (10 fmol), large binding capacity (100 μg mg−1) and satisfied enrichment recovery (approximately 82%). Furthermore, the newly developed Fe3O4@MPS@PMAC nanoparticles were applied for the glycopeptide enrichment of HeLa exosome proteins. A total of 1274 glycopeptides from 536 glycoproteins were identified in three replicate analyses of 50 μg of HeLa exosome proteins. These results demonstrate the potential of Fe3O4@MPS@PMAC nanoparticles for both glycoproteomic analysis and exosome research. |
url |
https://doi.org/10.1038/s41598-017-07516-x |
work_keys_str_mv |
AT fenglongjiao polymerichydrophilicionicliquidsusedtomodifymagneticnanoparticlesforthehighlyselectiveenrichmentofnlinkedglycopeptides AT fangyuangao polymerichydrophilicionicliquidsusedtomodifymagneticnanoparticlesforthehighlyselectiveenrichmentofnlinkedglycopeptides AT hepingwang polymerichydrophilicionicliquidsusedtomodifymagneticnanoparticlesforthehighlyselectiveenrichmentofnlinkedglycopeptides AT yulindeng polymerichydrophilicionicliquidsusedtomodifymagneticnanoparticlesforthehighlyselectiveenrichmentofnlinkedglycopeptides AT yangjunzhang polymerichydrophilicionicliquidsusedtomodifymagneticnanoparticlesforthehighlyselectiveenrichmentofnlinkedglycopeptides AT xiaohongqian polymerichydrophilicionicliquidsusedtomodifymagneticnanoparticlesforthehighlyselectiveenrichmentofnlinkedglycopeptides AT yukuizhang polymerichydrophilicionicliquidsusedtomodifymagneticnanoparticlesforthehighlyselectiveenrichmentofnlinkedglycopeptides |
_version_ |
1724393888370655232 |